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排序方式: 共有65条查询结果,搜索用时 15 毫秒
41.
Mutsuko Shirai Yoshichika Kawai Rintaro Yamanishi Takashi Kinoshita Hiroshi Chuman 《Free radical research》2013,47(10):1047-1053
To assess the efficacy of conjugated quercetin metabolites as attenuators for oxidative stress in the central nervous system, we measured the 13-hydroperoxyoctadecadienoic acid (13-HPODE)-dependent formation of reactive oxygen species (ROS) in pheochromocytoma PC-12 cells in the presence of quercetin 3-O-β-glucuronide (Q3GA) and related compounds. A 2′,7′-dichlorofluorescin (DCFH) assay showed that Q3GA significantly suppressed the formation of ROS, when it was coincubated with 13-HPODE (coincubation system). However, it was less effective than quercetin aglycon in the concentration range from 0.5 to 10 μM. In an experiment in which the cells were incubated with the test compounds for 24 h before being exposed to 13-HPODE, Q3GA was also effective in suppressing the formation of ROS in spite that little Q3GA was taken up into the cells. These results suggest that antioxidative metabolites of quercetin are capable of protecting nerve cells from attack of lipid hydroperoxides. 相似文献
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Liu Pengfei Chen Shubin Wang Yaofeng Chen Xiaoming Guo Yiping Liu Chunhua Wang Haitao Zhao Yifan Wu Di Shan Yongli Zhang Jian Wu Chuman Li Dongwei Zhang Yanmei Zhou Tiancheng Chen Yaoyu Liu Xiaobo Li Chenxu Wang Lihui Jia Bei Liu Jie Feng Bo Cai Jinglei Pei Duanqing 《中国科学:生命科学英文版》2021,64(12):2100-2113
Science China Life Sciences - A stable, rapid and effective neural differentiation method is essential for the clinical applications of human embryonic stem cells (ESCs) or induced pluripotent stem... 相似文献
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Yoshida T Shimizu M Harada M Hitaoka S Chuman H 《Bioorganic & medicinal chemistry letters》2012,22(1):124-128
The Hammett σ constant has for a long time been known to be one of most important linear free-energy related parameters that correlate with biological activity. It is a conventionally used electronic parameter in studies of enzymatic quantitative structure-activity relationships (QSAR). However, it is not necessarily obvious why σ represents variations in the free-energy change associated with the complex formation between a congeneric series of ligands with their target protein. So far, several powerful molecular calculations, such as the ab initio fragment molecular orbital (FMO) one, that are directly applicable to ligand-protein complexes have emerged. In this study, we comprehensively reevaluate experimentally derived parameter σ confirming it represents intermolecular interaction energy terms, by applying molecular orbital (MO) calculations to a simple ligand-protein complex model. The current results provide a rational and quantitative basis for bridging the gap between the traditional QSAR approach and 'the modern QSAR one', which involves the molecular calculations to evaluate the overall free-energy change for complex formation. 相似文献
46.
To understand the transport mechanism of the bovine heart mitochondrial ADP/ATP carrier at the atomic level, we studied the four-dimensional features of the interaction of various purine nucleotides with the adenine nucleotide binding region (ABR) consisting of Arg(151)-Asp(167) in the second loop facing the matrix side. After three-dimensional modeling of ABR based on the experimental results, its structural changes on interaction with purine nucleotides were examined by molecular dynamics computation at 300 K. ATP/ADP were translocated to a considerable degree from the matrix side to the inner membrane region accompanied by significant backbone conformational changes, whereas neither appreciable translocation nor a significant conformational change was observed with the untransportable nucleotides AMP/GTP. The results suggested that binding of the terminal phosphate group and the adenine ring of ATP/ADP with Arg(151) and Lys(162), respectively, and subsequent interaction of a phosphate group(s) other than the terminal phosphate with Lys(162) triggered the expansion and subsequent contraction of the backbone conformation of ABR, leading to the translocation of ATP/ADP. Based on a simplified molecular dynamic simulation, we propose a dynamic model for the initial recognition process of ATP/ADP with the carrier. 相似文献
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Ayami Matsushima Satoru Yokotani Xiaohui Lui Kazunori Sumida Takeshi Honda Seiji Sato Atsushi Kaneki Yukimasa Takeda Yoshiro Chuman Mamiko Ozaki Daisuke Asai Takeru Nose Hitoshi Onoue Yushi Ito Yoshiya Tominaga Yasuyuki Shimohigashi Miki Shimohigashi 《International journal of peptide research and therapeutics》2003,10(5-6):419-430
Pigment-dispersing factor PDF is an 18-amino acid insect neuropeptide that mediates a circadian rhythmicity in locomotor activity.
PDF is coded in a precursor protein together with another neuropeptide named PDF-associated peptide, PAP. PDF is highly conserved
among insects, whereas the homology of PAPs is very low with considerably varied amino acid sequences. Since such dissimilarity
has suggested that the function of PAP peptide is not associated with that of PDF, we have attempted to analyze the sequences
of PDF precursor proteins among a series of species of insects and hypothesized that PDF precursors are classified into at
least three different classes: Drosophila-Musca, Meimuna-Romalea, and Gryllus. In order to exemplify this hypothesis, we here describe the molecular cloning of the pdf-gene of the black blowfly Phormia regina and an in silico screening for the pdf-gene in the genome databank of the mosquito Anopheles gambie, both species belonging to the Diptera. It was found that deduced amino acid sequences of PDF peptides are almost completely
conserved among all Dipterans and also the amino acid sequences of PAPs are considerably highly preserved (55–82 similarity)
among the species of Diptera. The results confirmed the validity of grouping the PDF precursor proteins. In situ hybridization was carried out in fly brains to identify the precise locations of pdf-expressing cells and to examine any daily cycling of pdf mRNA. Intense signals for pdf mRNA were identified in the medulla, but not in the pars lateralis where PDF neurons were strongly immunostained by the antibody
raised against PDF peptide. Hybridization was also performed for the brain samples at two hour intervals throughout the day.
Although very intense hybridization signals were observed at ZT8 even in some neurites, no prominent rhythmicity of pdf mRNA expression was observed. 相似文献
49.
Chuman Y Iizuka K Honda T Onoue H Shimohigashi Y Sakaguchi K 《Journal of biochemistry》2011,150(3):319-325
Protein phosphorylation plays central roles in a wide variety of signal transduction pathways and most phosphorylated proteins contain multi-phosphorylated sites. PPM1 type Ser/Thr protein phosphatase family is known to show rigid substrate specificity unlike other Ser/Thr phosphatase PPP family including PP1, PP2A and PP2B. PPM1 type phosphatases are reported to play important roles in growth regulation and in cellular stress signalling. In this study, we developed a phosphatase assay of PPM1D using phosphatase motif-specific antibody. PPM1D is a member of PPM1 type Ser/Thr phosphatase and known to dephosphorylate Ser(P)-Gln sequence. The gene amplification and overexpression of PPM1D were reported in many human cancers. We generated the monoclonal antibody specific for the Ser(P)-Gln sequence, named 3G9-H11. The specificity of this method using ELISA enables the convenient measurement of the dephosphorylation level of only PPM1D target residues of substrate peptides with multiple phosphorylated sites in the presence of multiple phosphatases. In addition, the antibody was applicable to immunoblotting assay for PPM1D function analysis. These results suggested that this method should be very useful for the PPM1D phosphatase assay, including high-throughput analysis and screening of specific inhibitors as anti-cancer drugs. The method using phosphatase motif-specific antibody can be applied to other PPM1 phosphatase family. 相似文献
50.
Keiko Mochizuki Tatsuji Chuman Masataka Mori Masahiro Kohno Kunio KatŌ 《Bioscience, biotechnology, and biochemistry》2013,77(11):2833-2834
Formation of quinoprotein methanol dehydrogrnase (MDH) in Methylobacillus glycogenes, an obligate methylotroph, was strictly controlled by calcium (Ca) in the culture medium. Both the growth of the organism and the total enzyme activity of MDH were also repressed at less than 1 ppm of Ca, although specific activity of MDH showed a similar level. Ca in MDH was replaced with other metals during cultivation of M. glycogenes. When strontium (Sr) chloride was fed instead of CaCl2, Ca in MDH was completely replaced by Sr and showed a specific activity over ten times higher than Ca-containing MDH, although there was no appreciable increase in the MDH content in cells cultured in Sr medium. Methanol oxidase activity was also elevated in the cells that were cultured in the medium with Sr. 相似文献