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排序方式: 共有86条查询结果,搜索用时 31 毫秒
71.
Molecular characterization of two highly homologous receptor-like kinase genes, RLK902 and RKL1, in Arabidopsis thaliana 总被引:1,自引:0,他引:1
Tarutani Y Morimoto T Sasaki A Yasuda M Nakashita H Yoshida S Yamaguchi I Suzuki Y 《Bioscience, biotechnology, and biochemistry》2004,68(9):1935-1941
Receptor-like kinases (RLKs) constitute a large family of signal perception molecules. We characterized two highly homologous RLK genes, RLK902 and RKL1, in Arabidopsis. RLK902 and RKL1 showed a 75% amino acid sequence identity over their entire regions. In the RLK902 pro::GUS transgenic lines, GUS activity was strong in the root tips, lateral root primordia, stipules, and floral organ abscission zones, while the RKL1 promoter activity was dominant in the stomata cells, hydathodes and trichomes of young rosette leaves, and floral organ abscission zones. Neither the rlk902 mutant line, rkl1 mutant line nor rlk902/rkl1 double-knockout mutant line showed any significant phenotypes under normal growth conditions. These results suggest that RLK902 and RKL1 might mediate the signal transduction pathway in which at least one other complementary signaling pathway to these two RLKs might exist. 相似文献
72.
Fukuda A Nemoto K Chono M Yamaguchi S Nakajima M Yamagishi J Maekawa M Yamaguchi I 《Bioscience, biotechnology, and biochemistry》2004,68(8):1814-1816
Rice anthers contain high concentrations of gibberellins A(4) and A(7). To understand their physiological roles, we examined the site of their biosynthesis by analyzing the expression pattern of a gene (OsCPS) encoding coparyl diphosphate synthase in developing rice flowers. Expression was apparent in the anthers 1-2 days before flowering, and CPS mRNA accumulated in the maturing pollen. 相似文献
73.
74.
Differential expression and affinities of Arabidopsis gibberellin receptors can explain variation in phenotypes of multiple knock-out mutants 总被引:2,自引:0,他引:2
Hiroyuki Suzuki Seung-Hyun Park Kyoko Okubo Jun Kitamura Miyako Ueguchi-Tanaka Satoshi Iuchi Etsuko Katoh Masatomo Kobayashi Isomaro Yamaguchi Makoto Matsuoka Tadao Asami Masatoshi Nakajima 《The Plant journal : for cell and molecular biology》2009,60(1):48-55
In Arabidopsis, three receptors exist for the phytohormone gibberellin. Of the three, only a double loss-of-function mutant ( atgid1a atgid1c ) shows a dwarf phenotype, while other double and all single mutants show no abnormality in height. In this study we show that the expression of AtGID1b–GUS mRNA, driven by the AtGID1b promoter, is low in inflorescence stems, but may be 10% of AtGID1a–GUS mRNA, driven by the AtGID1a promoter. However, AtGID1b – GUS enzymatic activity does not exist in them. This factor strongly suggests that atgid1a atgid1c lacks sufficient AtGID1b protein for normal stem growth. In the stamens of pAtGID1c::AtGID1c–GUS transformants, we detected clear AtGID1c – GUS activity, while another atgid1a atgid1b , which has short stamens in its flowers, causes the adhesion of little pollen to stigmas thus leading to its low fertility. We then evaluated the affinity of the AtGID1 – DELLA interaction by a competitive yeast three-hybrid system and also by QCM apparatus. AtGID1c showed a quite lower affinity to RGL2, the major DELLA protein in floral buds, than AtGID1a or AtGID1b. The low affinity of the AtGID1c – RGL2 interaction is likely to be responsible for the failure of AtGID1c to hold RGL2, which is required for normal stamen development. Taken together with expressional information of DELLA genes, we propose that in a double loss-of-function mutant of gibberellin receptors, the emergence of any phenotype(s) depends on the abundance of the remaining receptor and its preference to DELLA proteins existing at a target site. 相似文献
75.
Isomaro Yamaguchi Masaharu Miyamoto Hisakazu Yamane Nobutaka Takahashi Kenichi Fujita Mamoru Imanari 《Bioscience, biotechnology, and biochemistry》2013,77(10):2453-2454
13C NMR spectroscopy was applied to studying lysine biosynthesis in Corynebacterium glutamicum ATCC 21543, a lysine producing mutant. It was cultured in a medium containing [1-13C]glucose or [6–13C]glucose as the sole carbon source and the 13C NMR spectrum of the culture filtrate was measured. C labeling patterns of l-lysine produced were well explained by the putative metabolic pathways of the bacterium. Fixation of 13CO2 liberated from the labeled substrates and the operation of the tricarboxylate cycle in the fermentation were obviously observed. The dual operations of the classical diaminopimelate pathway and the diaminopimelate dehydrogenase bypath were supported. Calculation of the contribution ratios of the metabolic pathways was attempted. 相似文献
76.
Yang Young-Yell; Yamaguchi Isomaro; Takeno-Wada Kiyotoshi; Suzuki Yoshihito; Murofushi Noboru 《Plant & cell physiology》1995,36(2):221-227
Gibberellin A1, (GA1), GA19, and GA20 in phloem exudates andcotyledons of seedlings of Pharbitis nil cv. Violet, grown underdifferent photoperiodic conditions, were qualitatively and semi-quantitativelyanalyzed by a combination of high performance-liquid chromatography(HPLC) and radioimmunoassays (RIA). The levels of GA19 and GA20were higher in cotyledons from plants grown under dark treatment(DT) conditons of 16 h-light/8 h-dark for 6 days followed by8 h-light/16 h-dark for 3 days than in those grown under continuouslight (CL) for 9 days. This relationship was also observed forthe GAs in phloem exudates, although the levels were much lowerthan in the cotyledons. When GAs were applied to the cotyledons,elongation of the epicotyl was promoted more by GA20 than byGA1 or GA19, especially under the CL treatment. The relativeeffect of GA1 and GA20 on the epicotyl elongation was reversedwhen these GAs were applied to epicotyls pre-treated with prohexadione,an inhibitor of 2-oxoglutarate-dependent dioxygenases.
3Present address: Frontier Research Program, The Institute ofPhysical and Chemical Research (RIKEN), 2-1 Hirosawa, Wakoshi,Saitama, 351-01 Japan
4Present address: Laboratory of Horticulture, Faculty of Agriculture,Nagoya University, Nagoya, 464-01 Japan 相似文献
77.
Nakajima Masatoshi; Yamaguchi Isomaro; Kizawa Satoru; Murofushi Noboru; Takahashi Nobutaka 《Plant & cell physiology》1991,32(4):511-513
The levels of endogenous GA, and GA4 in the anthers of a standardcultivar and the corresponding male-sterile, single gene mutantof rice, Oryza saliva L. (Japonica), were measured by radioimmunoassayusing GA4-specific antiserum that showed highly specific immunoreactivitywith GA, and GA4. The levels of these GAs in the anthers ofthe male-sterile mutant were about one-fifth to one-sixth ofthose in the normal cultivar, suggesting a correlation betweenthe endogenous levels of these GAs and the male sterility.
2Present address: Department of Agricultural Chemistry, UtsunomiyaUniversity, Mine-machi 350, Utsunomiya, 321 Japan (Received August 8, 1990; Accepted March 7, 1991) 相似文献
78.
Fujioka Shozo; Yamaguchi Isomaro; Murofushi Noboru; Takahashi Nobutaka; Kaihara Sumiko; Takimoto Atsushi; Cleland Charles F. 《Plant & cell physiology》1986,27(1):109-116
Nicotinic acid induces flowering in Lemna paucicostata 151 and381 and Lemna gibba G3 when they are grown in one tenth-strengthM medium under continuous light. For L. paucicostata 151 and381, the simultaneous addition of IAA, GA3 or ABA to the mediumleads to an inhibition of the flower-inducing effect of nicotinicacid, while zeatin leads to a further stimulation of floweringabove that obtained by nicotinic acid alone. By contrast, inL. gibba G3 all four plant hormones inhibit the nicotinic acid-inducedstimulation of flowering. The effect of nicotinic acid on flowering in all three plantsis strongly daylength dependent when the plants are grown inhalf-strength Hutner's medium. Thus, nicotinic acid causes floweringin L. gibba G3 on continuous light but not on 9L:15D or 10L:14Dregimes. In L. paucicostata 381 nicotinic acid has a small effecton 12L:12D regime, a large effect on a 13L:11D regime and noeffect with daylengths longer than 14 hours, and in L. paucicostata151 nicotinic acid is only effective on daylengths shorter thanabout 11 hours. However, in L. paucicostata 151 and 381 treatmentwith both nicotinic acid and zeatin results in flowering undercontinuous light on half-strength Hutner's medium. Nicotinic acid is present in different Lemna but its concentrationdoes not appear to be influenced by changes in daylength. Thus,flowering clearly cannot be controlled by nicotinic acid actingalone, but the results of this study indicate that nicotinicacid could interact with other factors, possibly including oneor more of the known plant hormones, to influence the floweringprocess in Lemna. (Received August 28, 1985; Accepted October 29, 1985) 相似文献
79.
Shozo Fujioka; Isomaro Yamaguchi; Murofushi Noboru; Takahashi Nobutaka; Kaihara Sumiko; Takimoto Atsushi 《Plant & cell physiology》1983,24(2):241-246
The effects of plant hormones on flowering of Lemna paucicostata151 and 381 were investigated when exogenously applied in combinationwith benzoic acid. These strains are very sensitive to benzoicacid and flower readily on application of benzoic acid. Theflower-inducing effect of benzoic acid was strongly modifiedby plant hormones: gibberellins, indole-3-acetic acid and abscisicacid were inhibitory, while cytokinins were promotive (synergistic),suggesting that the balance between endogenous levels of benzoicacid and plant hormones contributes to the regulation of floweringin Lemna. (Received October 6, 1982; Accepted December 23, 1982) 相似文献
80.
Suzuki Yoshihito; Yamaguchi Isomaro; Murofushi Noboru; Takahashi Nobutaka 《Plant & cell physiology》1988,29(3):445-448
The flower-inducing activities in Lemna paucicostata 151 offour major metabolites of benzoic acid (N-benzoyl aspartate,benzyl 6-O-ß-D-apiofuranosyl-O-ß-D-glucopyranoside,O-benzoyl isocitrate and O-benzoyl malate) were measured, andthe effects on the uptake and metabolism of benzoic acid dueto change in the level of the benzoic acid concentration orto the addition of plant hormones were investigated. N-Benzoylaspartate had weak activity, and O-benzoyl isocitrate and malatehad fairly strong activities, while benzyl 6-O-ß-D-apiofuranosyl-ß-D-glucopyranosideshowed no activity. As the concentration of benzoic acid rose,the ratio of N-benzoyl aspartate increased and that of benzyl6-O-ß-D-apiofuranosyl-O-ß-D-glucopyranosidedecreased. GA3 and IAA, inhibitors of flower induction by benzoicacid, seemed to promote conversion to N-benzoyl aspartate insteadof to benzyl 6-O-ß-D-apiofuranosyl-ß-D-glucopyranoside.The conversion to N-benzoyl aspartate was considered to be adetoxification process and that to benzyl 6-O-ß-D-apiofuranosyl-ß-D-glucopyranosidemay be directly related to flower induction in Lemna. (Received November 2, 1987; Accepted January 23, 1988) 相似文献