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51.
Mark J. Wagner Joan Savall Oscar Hernandez Gabriel Mel Hakan Inan Oleg Rumyantsev Jérôme Lecoq Tony Hyun Kim Jin Zhong Li Charu Ramakrishnan Karl Deisseroth Liqun Luo Surya Ganguli Mark J. Schnitzer 《Cell》2021,184(14):3731-3747.e21
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52.
A numerical taxonomy was performed on 157 cultures (141 different strains) of species of Bacteroides, Polyphyromonas, Prevotella [not Prevotella (Labroue, 1976)] and Fusobacterium. Isolates were each tested for 111 phenotypic characters which included possession of constitutive enzymes, fermentation of specific carbohydrates, gas chromatographic analysis of metabolic end-products and of cellular carboxylic acid composition. Computation of similarity coefficients was followed by a single-linkage cluster analysis. At the 94% similarity level, the following groupings at genus level were apparent: (1) Bacteroides ureolyticus; (2) Fusobacterium mortiferum, F. necrogenes, F. necrophorum, F. nucleatum and F. varium; (3) B. caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B stercoris, B. thetaiotaomicron, B. uniformus and B. vulgatus; (4) B. splanchnicus; (5) Porphyromonas asaccharolytica; (6) B. bivius (Prevotella bivia); (7) B. disiens (P. disiens); (8) B. intermedius (P. intermedia);and (9) B. melaninogenicus (P. melaninogenica). Single isolates of B. ruminicola (P. ruminicola), B. denticola (P. denticola) and B. capillosus did not cluster with other strains. 相似文献
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54.
Hamycin, a polyene antifungal antibiotic, was isolated by use of countercurrent distribution. A pattern was obtained by plotting the absorption at 383 mmu of the contents of the various tubes against the tube numbers. The results indicated that the antibiotic contained three fractions, a major fraction (peak 2) comprising 48% of the total activity and two minor fractions (peak 1 and peak 3) comprising 3.62 and 11.32%, respectively, of the total activity. The solid material was isolated by pooling the contents of the tubes containing the major fraction, concentrating this in vacuo, and cooling. The antibiotic activities of the three peaks were evaluated by use of a cup-plate assay method with Paecilomyces varioti as test organism. All three components showed antibiotic activity; however, the preparation obtained from the major fraction showed approximately a 7-fold increase in antibiotic activity, a doubling of the E(1cm) (1%) value at 383 mmu, and approximately a 2.5-fold decrease in the amino acid content in comparison with the starting material. There was an apparent correlation obtained by plotting the curves of the absorption at 383 mmu of the different tubes comprising the major fraction and their biological activities. 相似文献
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56.
Human blood reduced the numbers of colony-forming units (cfu) of Candida albicans in a blood-culture model so that the detection time was increased by 12 h. Reduction in cfu was accompanied not by reduction in cell mass but by observable clumping of cultures, which was attributable to a heat-stable serum component. The action of the latter component could be negated if the medium were supplemented by a combination of trypsin, 2-phenyl ethanol, liquoid and Tween 80, when a statistically significant improvement was noted in minimum detection time for C. albicans growing in blood-culture medium. 相似文献
57.
The deviation of the static weight-bearing patterns under the feet of a lower extremity handicapped person may be measured
quantitatively in terms of the Static Weight-Bearing Index. This paper describes the correlation between this index and the
physiological energy expenditure associated with the erect standing posture. The Static Weight-Bearing Index can be conveniently
used for evaluation of the functional status of the human lower extremity system in stance in case of lower extremity disabled
persons who maintain basic weight-bearing mode involving both the limbs in double support condition. 相似文献
58.
59.
V.N. Purandare A.C. Ganguli R.M. Gharse U.R. Krishna 《Prostaglandins & other lipid mediators》1977,13(2):349-354
Midtrimester abortions were induced in 796 women by Hypertonic Saline and Prostaglandins. Intra-amniotic and Extra-amniotic routes were used for saline and Intra-amniotic, Extra-amniotic and Intra-muscular routes were used for Prostaglandins. Seven patients had cervico-vaginal injuries. All were young nulliparous patients. None of them developed any signs and symptoms of shock. Five out of the seven injuries were posteriorly situated. The etio-pathology and preventive measures of such injuries are discussed here. 相似文献
60.
Seghatoleslam A Zambrano A Millon R Ganguli G Argentini M Cromer A Abecassis J Wasylyk B 《Biochemical and biophysical research communications》2006,339(1):422-429
We have identified by differential display a number of novel genes that are expressed in hypopharyngeal head and neck squamous cell carcinoma. We report here the characterisation of one of these novel human genes, LOC92912, that encodes a protein of 375 amino acids. The protein contains a RWD domain, a coiled-coil, and an E2 ubiquitin conjugating enzyme domain. LOC92912 is upregulated in about 85% of tumour samples. It is expressed in tumour masses and in invasive epithelium, and is located in the cytoplasm of cells. To gain insights into its functions, we identified potential interacting partners by immunoaffinity purification of the flag tagged protein followed by MALDI peptide mass fingerprinting mass spectrometry. Actin and six actin-binding proteins were unambiguously identified as potential interacting partners, suggesting that LOC92912's functions may be linked with the cytoskeleton. This novel human gene may represent a new target for cancer therapeutics. 相似文献