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191.
Skewing of the sex ratio towards males occurs in humans. The possible explanation for excess male births could be a preference for Y-bearing sperm at fertilization and/or selective elimination of female embryos during pregnancy. In this study, we have tested the sex ratio in the preimplantation embryo (2–3 cells stage/closest possible primary sex ratio), the post-implantation embryo (day E7.5), and at birth (secondary sex ratio) on a homogenous (genetic, environmental, and dietary) population of mice to ascertain the biological reason i.e., male preference at fertilization or female elimination during pregnancy or both. Primary sex ratio on early preimplantation embryos (2–3 cells stage) was studied on 598 embryos and secondary sex ratio (at birth) on 721 pups using PCR-based sexing (both X & Y chromosome-specific) besides sex ratio of 80 post-implantation embryos (day E7.5). We have also investigated whether the fat content (high & low) of the diet affects the sex ratio. We observed a skewed sex ratio (more female) in preimplantation embryos (0.436; 95 % CI 0.39, 0.48), and post-implantation embryos (0.462; 95 % CI 0.35, 0.57) but reverse skewing (more male) at birth (0.539; 95 % CI 0.5, 0.58). We also observed that high-fat diet promoted male sex ratio at birth (0.657; 95 % CI 0.57, 0.74) whereas a low-fat diet had the opposite effect (0.46; 95 % CI 0.36, 0.56) but no effect at fertilization (2–3 cells stage embryos). This indicates selective elimination of female embryo and fetus throughout pregnancy in mice, more so with a high-fat diet. 相似文献
192.
Alpha-chymotrypsin was made more hydrophilic by modifying 11 (out of 16) ε-amino groups with pyromellitic dianhydride. The hydrophilic preparation was precipitated with n-propanol. This preparation gave significantly higher initial rates at the optimum aw (127.51 nmol mg?1 min?1 in n-octane and 21.30 nmol mg?1 min?1 in acetonitrile at aw=0.33) compared with the lyophilized preparation (53.50 nmol mg?1 min?1 in n-octane and 0.26 nmol mg?1 min?1 in acetonitrile at aw=0.97). FT-IR showed that the precipitate of modified alpha-chymotrypsin has a higher content of alpha-helices and beta-sheets compared to the lyophilized powder. 相似文献
193.
Glycine (50, 100 and 300 mg/kg), administered daily for 10 days in rabbits challenged with typhoid 'H' antigen and sheep erythrocyte antigen, caused dose- dependent reduction of antibody titre. Inhibition of antibody titre observed with 300 mg/kg was comparable to immunosuppression observed with 1 mg/kg betamethasone. 相似文献
194.
Western India experiences arid to dry subhumid ecoclimate. Aridity increases from east to west to the Great Indian Desert or Thar Desert. The desert economy is animal based and goats are the most common livestock; density of goats increases with increase in aridity. There are large morphological variations in goats warranting the recognition of population differences throughout the region. The present investigations were therefore undertaken to see whether there are population differences and, if so, to establish the populations on a sound ecological basis. Eleven physical measurements of the animals were made in the fields of western India in a total of 518 animals (255 adults). Five populations were initially segregated by means of a scatter diagram. Population differences were confirmed by mathematical treatments using analysis of variance (one-way and two-way) and Students-Newman-Keuls test, which yields a critical comparison among means. Discriminant analysis was used to determine whether the goat populations differed significantly between the five climo-edaphic-vegetational zones. Each population was designated according to the locality having the greatest density as: POP1Ku, POP2Sa, POP3Jh, POP4Ga, POP5Pa. The five populations occurring in distinct climo-edaphic-vegetational zones or ecological niches in western India may be referred to as ecotypes. 相似文献