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791.
792.
Motoki Endo Yosuke Inoue Keisuke Kuwahara Chihiro Nishiura Ai Hori Takayuki Ogasawara Miwa Yamaguchi Tohru Nakagawa Toru Honda Shuichiro Yamamoto Hiroko Okazaki Teppei Imai Akiko Nishihara Toshiaki Miyamoto Naoko Sasaki Akihiko Uehara Makoto Yamamoto Taizo Murakami Makiko Shimizu Masafumi Eguchi Takeshi Kochi Satsue Nagahama Kentaro Tomita Naoki Kunugita Takeshi Tanigawa Maki Konishi Akiko Nanri Isamu Kabe Tetsuya Mizoue Seitaro Dohi 《Obesity (Silver Spring, Md.)》2020,28(2):437-444
793.
High-performance liquid chromatographic analysis of α-keto acids produced from amino acid metabolism in oral Bacteroides 总被引:1,自引:0,他引:1
H. Tsuchiya †M. Sato ‡K. Yamamoto §M. Yamauchi ¶H. Tani ‡I. Namikawa ‡N. Takagi † 《Journal of applied microbiology》1990,69(1):125-133
Profiles of metabolic α-keto acids were determined by a high-performance liquid chromatographic method and applied to characterization of oral black-pigmented Bacteroides . Each bacterial strain was incubated with amino acids in a chemically defined medium. After production α-keto acids were purified by hydrazide gel column treatment and converted to u.v.-absorbing derivatives. They were analysed by reversed-phase ion-pair chromatography. Black-pigmented Bacteroides species were differentiated into two groups according to production of aromatic α-keto acids. Bacteroides gingivalis, B. endodontalis and B. loescheii produced both ρ-hydroxyphenylpyruvic and phenylpyruvic acids. However, no such α-keto acids were produced by B. levii, B. intermedius and B. denticola . In addition, production profiles of several aliphatic α-keto acids (α-ketoglutaric, pyruvic, α-ketobutyric, α-ketoisovaleric, α-ketoisocaproic, and α-keto-β-methylvaleric acids) separated each individual species in such groups. The present study offers useful chemotaxonomic information on amino acid metabolic activity of oral black-pigmented Bacteroides species. 相似文献
794.
795.
796.
Ammonia or Ammonium Ion as Substrate for Oxidation by Nitrosomonas europaea Cells and Extracts 总被引:12,自引:2,他引:10
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The effect of pH on the K(m) values for ammonia was studied in its oxidation by Nitrosomonas cells and cell-free extracts. The K(m) values decreased markedly with increasing pH, suggesting (NH(3)) rather than (NH(4) (+)) as the actual substrate for oxidation. 相似文献
797.
798.
H Fushimi T Inoue H Namikawa B Kishino H Nunotani M Nishikawa Y Tochino S Funakawa A Yamatodani H Wada 《Endocrinologia japonica》1982,29(5):593-596
Previously we reported that the heart norepinephrine concentration was markedly increased in diabetic rats. To further study the relationship between a disturbance in the autonomic nervous system and catecholamine metabolism in diabetes mellitus, the plasma catecholamine response to stress and catecholamine concentration of heart and adrenals were measured. Wistar male rats were made diabetic by streptozotocin and kept for 13 weeks. A silicon catheter was placed in the superior V. cava 1 week prior to the experiment. Insulin was injected subcutaneously for 3 days once daily. After an overnight fast and without anesthesia, 1 ml of blood, a control sample, was obtained and then the animals were exsanguinated. The blood was mixed with 1 mM EGTA at a final concentration and centrifuged. The tissue was homogenized with 0.4 N perchloric acid containing 1 mM EGTA and centrifuged at 10,000 x g for 20 minutes. Catecholamines were determined by high performance liquid chromatography. Normal rats responded to blood withdrawal stress, and plasma catecholamines were markedly increased, but almost no increase or an actual decrease was observed in diabetic rats. These abnormal responses were improved by insulin treatment. Heart norepinephrine was increased significantly in the diabetic rats compared with the control rats and was reduced significantly by insulin injections. Adrenal epinephrine was also significantly increased in the diabetic rats compared with the control rats, but was not significantly reduced by insulin. These result suggest a possible disturbance of catecholamine secretion in the diabetic rats. 相似文献
799.
5-Aminolevulinic acid (ALA) accumulation in dark-grown tobaccocallus cells in the presence of levulinic acid (LA) was followedunder blue or red light or in continuous darkness. Significantformation of ALA continued in the dark. The protochlorophyll-(ide) (Pchl) content of dark-incubated cells remained low becauseof its turnover. We inferred that the feedback inhibition ofALA synthesis by Pchl would not occur in darkincubated calluscells. ALA formation was enhanced by blue light, and this effectreached saturation at an intensity of about 800 mW.m2.Neither weak nor strong red light affected ALA formation. Fullenhancement of ALA formation by blue light was attained afterfairly long continuous illumination of the callus cells. Thisblue lightenhanced activity of ALA synthesis declined very slowlyduring the subsequent dark incubation. The blue light enhancement of ALA formation was observed incallus cells supplied with sucrose over a wide range of concentrations.Pchl regeneration in carbon-starved callus cells, supplied withglutamate at various concentrations, was also markedly enhancedby blue light. Respiration of the callus cells was not enhancedby blue light. A possible role of blue light in regulating ALAformation in callus cells is discussed.
1Dedicated to the late Professor Joji Ashida. (Received September 3, 1982; Accepted April 5, 1983) 相似文献