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31.
High frequency of polyandry in a lek mating system 总被引:1,自引:0,他引:1
Lank David B.; Smith Constance M.; Hanotte Olivier; Ohtonen Arvo; Bailey Simon; Burke Terry 《Behavioral ecology》2002,13(2):209-215
The adaptive significance of polyandry by female birds in theabsence of direct benefits remains unclear. We determined thefrequencies of polyandrous mating and multiple paternity inthe ruff, a lekking shorebird with a genetic dimorphism inmale mating behavior. More than half of female ruffs mate with,
and have clutches fertilized by, more than one male. Individualfemales mate with males of both behavioral morphs more oftenthan expected. Polyandrous mating was more likely followingcopulation interference, but interference was uncommon. Themultiple paternity rate of ruffs is the highest known for avian
lekking species and for shorebirds. The general hypothesis thatpair-bond constraints are the major selective factor favoringmultiple mating in birds does not predict our findings. Activegenetic diversification, which has been widely dismissed asa functional explanation for polyandrous mating in birds, mayapply with respect to the behavioral polymorphism in ruffs becauseof a Mendelian genetic basis for male behavioral morph determinationand aspects of malemale cooperation and female choice.However, rates of multiple paternity in other species of lekkingbirds are higher than generally realized, and the potentialbenefits of diversification in general deserve further consideration. 相似文献
32.
Tony Robinet Eric Feunteun Philippe Keith Gérard Marquet Jean-Michel Olivier Elodie Réveillac Pierre Valade 《Environmental Biology of Fishes》2007,78(2):93-105
Anguillid eels were sampled from permanent rivers in the Réunion and Mauritius islands, western Indian Ocean, with a standardized
electrofishing method. A. marmorata was very dominant, corresponding to 91.7 and 90.7% of all the eels collected in Réunion and Mauritius, respectively. Three
other species (A. mossambica, A. bicolor bicolor and A. nebulosa labiata) were also present in both islands. A. marmorata showed a strong altitudinal gradient of densities from the lower to upper zones, especially in the younger stages (TL <250 mm),
while A. mossambica was only found in the upper zones and A. bicolor bicolor occurred only in the lower zones (A. nebulosa labiata was rare). The eel species composition in freshwaters of both islands is very similar because these two adjoining islands
are located in the same trail of drifting marine larvae. Mean estimated eel biomasses were noticeably low (11.1 and 22.2 kg ha−1 in Réunion and Mauritius islands, respectively), especially when compared to those of other tropical insular systems without
any eel fishery (Comoros or Polynesia, more than 100 kg ha−1). Nevertheless, the fluvial recruitment of A. marmorata seemed to be regular during the surveyed period, staggering from October to April. The obvious lack of large eels in Mauritius
but more significantly in Réunion suggests a high pressure from traditional fishery, and the local reproductive turnover is
uncertain. Because sexual maturation seems to occur at a large body size for A. marmorata, as for temperate species, the Réunion and Mauritius rivers may only have a weak contribution to the regional production
of spawners. However, the giant mottled eel population in the western Indian Ocean is believed to be panmictic at the regional
scale, and may not rely exclusively on these islands’ contribution. A comparison is made with those of freshwater systems
in other tropical islands. 相似文献
33.
Isabelle Larocque-Tobler Martin Grosjean Christian Kamenik 《Palaeogeography, Palaeoclimatology, Palaeoecology》2011,299(1-2):281-288
Calibration-in-space (i.e. modern taxonomic assemblages of biota from many lakes located along a wide temperature gradient calibrated against meteorological data) is generally used to derive species-specific optima and tolerances. This results in transfer functions which then are applied to subfossil assemblages to quantitatively reconstruct environmental variables such as air/water temperature. Developing such transfer functions is time- and money-consuming, thus many biota-inferred temperature records are either based on transfer functions from other regions which might not take into account local characteristics or are only used qualitatively. In varved Lake Silvaplana (Engadine, Switzerland), another way of obtaining quantitative climate reconstructions from taxonomical assemblages preserved in lake sediments was assessed for the past 1000 years. A calibration-in-time (i.e. taxonomic-assemblage-of-biota time series calibrated against meteorological data covering the same time period) was developed for chironomids (non-biting midges) using a weighted-average-partial-least-square (WAPLS) model and compared with a calibration-in-space model. The calibration-in-time had a weaker correlation coefficient (r2 = 0.71) than the calibration-in-space (r2 = 0.86), but the error of prediction (RMSEP = 0.58 °C) and the maximum bias (Max Bias = 0.73 °C) outperformed the statistics of the calibration-in-space (RMSEP = 1.5 °C; Max Bias = 1.72). This result is probably due to the smaller temperature gradient of the calibration-in-time (6.5 °C) than the calibration-in-space (11.5 °C). For the last 150 years, the Pearson correlation coefficient was significant between the two reconstructions (rPearson = 0.52; p < 0.01) suggesting that both models recorded a similar pattern of temperature changes. On the millennium time-scale, both models showed a warm “Medieval Climate Anomaly”, a cold “Little Ice Age” and a warming through the present with significant correlations (rPearson corrected for autocorrelation (corr) = 0.61, p < 0.01) until ca. 1780 AD and between ca. 1937 and 2000 AD (rPearson corr = 0.90, p < 0.01). The reconstructions using both models significantly diverged between ca. 1780 and 1937 AD (rPearson corr = ?0.47, p < 0.01). The results of both reconstruction methods were compared with four independent local and regional records of early instrumental and documentary data during the period of divergence. Both reconstructions showed similarities with the early instrumental/documentary records, thus it was inconclusive which of the reconstruction models provides the better estimates. However, these results suggest that a calibration-in-time can be used to reconstruct climate over the last 1000 years when no calibration-in-space is available. 相似文献
34.
In vivo importance of homologous recombination DNA repair for mouse neural stem and progenitor cells
Rousseau L Etienne O Roque T Desmaze C Haton C Mouthon MA Bernardino-Sgherri J Essers J Kanaar R Boussin FD 《PloS one》2012,7(5):e37194
We characterized the in vivo importance of the homologous recombination factor RAD54 for the developing mouse brain cortex in normal conditions or after ionizing radiation exposure. Contrary to numerous homologous recombination genes, Rad54 disruption did not impact the cortical development without exogenous stress, but it dramatically enhanced the radiation sensitivity of neural stem and progenitor cells. This resulted in the death of all cells irradiated during S or G2, whereas the viability of cells irradiated in G1 or G0 was not affected by Rad54 disruption. Apoptosis occurred after long arrests at intra-S and G2/M checkpoints. This concerned every type of neural stem and progenitor cells, showing that the importance of Rad54 for radiation response was linked to the cell cycle phase at the time of irradiation and not to the differentiation state. In the developing brain, RAD54-dependent homologous recombination appeared absolutely required for the repair of damages induced by ionizing radiation during S and G2 phases, but not for the repair of endogenous damages in normal conditions. Altogether our data support the existence of RAD54-dependent and -independent homologous recombination pathways. 相似文献
35.
36.
Bottone MG Soldani C Tognon G Gorrini C Lazzè MC Brison O Ciomei M Pellicciari C Scovassi AI 《Experimental cell research》2003,290(1):49-59
Paclitaxel affects microtubule stability by binding to beta-tubulin, thus leading to cell accumulation in the G(2)/M phase, polyploidization, and apoptosis. Because both cell proliferation and apoptosis could be somehow regulated by the protooncogene c-myc, in this work we have investigated whether the c-myc amplification level could modulate the multiple effects of paclitaxel. To this aim, paclitaxel was administered to SW613-12A1 and -B3 human colon carcinoma cell lines (which are characterized by a high and low c-myc endogenous amplification level, respectively), and to the B3mycC5 cell line, with an enforced exogenous expression of c-myc copies. In this experimental system, we previously demonstrated that a high endogenous/exogenous level of amplification of c-myc enhances serum deprivation- and DNA damage-induced apoptosis. Accordingly, the present results indicate that a high c-myc amplification level potentiates paclitaxel cytotoxicity, confers a multinucleated phenotype, and promotes apoptosis to a great extent, thus suggesting that c-myc expression level is relevant in modulating the cellular responses to paclitaxel. We have recently shown in HeLa cells that the phosphorylated form of c-Myc accumulates in the nucleus, as distinct nucleolar and extranucleolar spots; here, we demonstrated that, after the treatment with paclitaxel, phosphorylated c-Myc undergoes redistribution, becoming diffused in the nucleoplasm. 相似文献
37.
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39.
Julien Lagarde Romain Valabrègue Jean-Christophe Corvol Fanny Pineau Isabelle Le Ber Marie Vidailhet Bruno Dubois Richard Levy 《PloS one》2013,8(11)
Progressive supranuclear palsy (PSP) and frontotemporal lobar degeneration (FTD) are two clinicohistological entities that share a severe prefrontal syndrome. To what extent do the cognitive syndrome and the location of the underlying brain atrophy unify or segregate these entities? Here, we examined the clinical and radiological patterns of frontal involvement and the neural bases of the cognitive dysfunctions observed in the Richardson form of PSP and the behavioral variant of FTD (bvFTD). The cognitive profile and grey and white matter volume of PSP (n = 19) and bvFTD (n = 16) patients and control participants (n = 18) were compared using a standard battery of neuropsychological tests and voxel-based morphometry (VBM), respectively. Analyses of correlations between neuropsychological and morphometric data were additionally performed. The severity and qualitative pattern of cognitive dysfunction was globally similar between the two patient groups. Grey matter volume was decreased in widespread frontal areas and in the temporal uncus in bvFTD, while it was decreased in the frontal and temporal lobes as well as in the thalamus in PSP. We also found an unexpected involvement of the frontal rectal gyrus in PSP patients compared to controls. Correlation analyses yielded different results in the two groups, with no area showing significant correlations in PSP patients, while several frontal and some temporal areas did so in bvFTD patients. In spite of minor neuropsychological and morphological differences, this study shows that the patterns of cognitive dysfunction and atrophy are very similar in PSP and bvFTD. However, executive dysfunction in these diseases may stem from partially divergent cortical and subcortical neural circuits. 相似文献
40.
A novel procedure for genotyping of single nucleotide polymorphisms in trisomy with genomic DNA and the invader assay 下载免费PDF全文
Individuals with trisomy 21 display complex phenotypes with differing degrees of severity. Numerous reliable methods have been established to diagnose the initial trisomy in these patients, but the identification and characterization of the genetic basis of the phenotypic variation in individuals with trisomy remains challenging. To date, methods that can accurately determine genotypes in trisomic DNA samples are expensive, require specialized equipment and complicated analyses. Here we report proof-of-concept results for an Invader® assay-based genotyping procedure that can determine SNP genotypes in trisomic genomic DNA samples in a simple and cost-effective manner. The procedure requires only two experimental steps: a real-time measurement of the fluorescent Invader® signal and analysis with a specifically designed clustering algorithm. The approach was tested using genomic DNA samples from 23 individuals with trisomy 21, and results were compared to genotypes previously determined with pyrosequencing. Additional assays for 15 SNPs were tested in a set of 21 DNA samples to assess assay performance. Our method successfully identified the correct SNP genotypes for the trisomic genomic DNA samples tested, and thus provides an alternative to determine SNP genotypes in trisomic DNA samples for subsequent association studies in patients with Down syndrome and other trisomies. 相似文献