Adaptive and non‐adaptive evolution of trait means and genetic trait correlations for herbivory resistance and performance in an invasive plant

The EICA‐hypothesis predicts that invading plants adapt to their novel environment by evolving increased performance and reduced resistance in response to the release from natural enemies, and assumes a resource allocation tradeoff among both trait groups as mechanistic basis of this evolutionary change. Using the plant Silene latifolia as a study system, we tested these predictions by investigating whether 1) invasive populations evolved lower resistance and higher performance, 2) this evolutionary change is indeed adaptive, and 3) there is a negative genetic correlation between performance and resistance (i.e. a tradeoff) in native and introduced individuals. Moreover, we sampled eight native and eight invasive populations and determined their population co‐ancestry based on neutral SSR‐markers. We performed controlled crossings to produce five sib‐groups per population and exposed them to increased and reduced levels of enemy attack in a full‐factorial experiment to estimate performance and resistance. With these data, we performed trait‐by‐trait comparisons between ranges with ‘animal models’ that account for population co‐ancestry to quantify the amount of variance in traits explained by non‐adaptive versus adaptive evolution. Moreover, we tested for genetic correlations among performance and resistance traits within sib‐groups. We found significant reductions in resistance and increases in performance in invasive versus native populations, which could largely be attributed to adaptive evolution. While we detected a non‐significant trend towards negative genetic performance × resistance correlations in native populations, invasive populations exhibited both significant and non‐significant positive correlations. In summary, these results do not support a shift of performance and resistance trait values along a tradeoff line in response to enemy release, as predicted by EICA. They rather suggest that the independent evolution of both traits is not constrained by a tradeoff, and that various selective agents (including resource availability) interact in shaping both traits and in weakening negative genetic correlations in the invaded habitat.     

Intra- and interspecific hybridization in invasive Siberian elm

Hybridization creates unique allele combinations which can facilitate the evolution of invasiveness. Frequent interspecific hybridization between the Siberian elm, Ulmus pumila, and native elm species has been detected in the Midwestern United States, Italy and Spain. However, Ulmus pumila also occurs in the western United States and Argentina, regions where no native elm species capable of hybridizing with it occurs. We examined whether inter- or intraspecific hybridization could be detected in these regions. Nuclear markers and the program STRUCTURE helped detect interspecific hybridization and determine the population genetic structure in both the native and the two non-native ranges. Chloroplast markers identified sources of introduction into these two non-native ranges. No significant interspecific hybridization was detected between U. pumila and U. rubra in the western United States or between U. pumila and U. minor in Argentina and vice versa. However, the genetic findings supported the presence of intraspecific hybridization and high levels of genetic diversity in both non-native ranges. The evidence presented for intraspecific hybridization in the current study, combined with reports of interspecific hybridization from previous studies, identifies elm as a genus where both inter- and intraspecific hybridization may occur and help maintain high levels of genetic diversity potentially associated with invasiveness.     

Eco-efficiency analysis by basf: the method

Intention, Goal, Scope, Background  BASF has developed the tool of eco-efficiency analysis to address not only strategic issues, but also issues posed by the marketplace, politics and research. It was a goal to develop a tool for decision-making processes which is useful for a lot of applications in chemistry and other industries. Objectives. The objectives were the development of a common tool, which is usable in a simple way by LCA-experts and understandable by a lot of people without any experience in this field. The results should be shown in such a way that complex studies are understandable in one view. Methods  The method belongs to the rules of ISO 14040 ff. Beyond these life cycle aspect costs, calculations are added and summarized together with the ecological results to establish an eco-efficiency portfolio. Results and Discussion  The results of the studies are shown in a simple way, the eco-efficiency portfolio. Therefore, ecological data are summarized in a special way as described in this paper. It could be shown that the weighting factors, which are used in our method, have a negligible impact on the results. In most cases, the input data have an important impact on the results of the study. Conclusions. It could be shown that the newly developed eco-efficiency analysis is a new tool, which is usable for a lot of problems in decision-making processes. It is a tool which compares different alternatives of a defined customer benefit over the whole life cycle. Recommendations and Outlook  This new method can be a helpful tool in different fields of the evaluation of product or process alternatives. It can be used in research and development as well as in the optimization of customer processes and products. It is an analytical tool for getting more sustainable processes and products in the future     

Is <Emphasis Type="Italic">Campanula glomerata</Emphasis> Threatened by Competition of Expanding Grasses?

In East Germany, a high percentage of species-rich, semi-natural dry grasslands has been converted into species-poor communities dominated either by Poa angustifolia or Festuca rupicola. The disappearance of low-intensity types of agricultural land-use such as mowing and grazing has been the cause for the decline of many grassland species, as lack of biomass extraction leads to a new situation in competition. Here we evaluate the performance of the rare forb Campanula glomerata in competition with P. angustifolia and F. rupicola using a replacement design experiment. C. glomerata responded to the presence of these grass species in contrasting ways: whereas the presence of P. angustifolia had a negative effect on C. glomerata biomass and fitness, the presence of F. rupicola affected the forb in a positive way. The results of an additional sowing experiment revealed that the germination and establishment rates of C. glomerata decrease in accordance with increasing density of P. angustifolia. Thus the response of this rare forb varies among neighbour species.     

A novel copper-binding fold for the periplasmic copper resistance protein CusF

We have determined the crystal structure of apo-CusF, a periplasmic protein involved in copper and silver resistance in Escherichia coli. The protein forms a five-stranded beta-barrel, classified as an OB-fold, which is a unique topology for a copper-binding protein. NMR chemical shift mapping experiments suggest that Cu(I) is bound by conserved residues H36, M47, and M49 located in beta-strands 2 and 3. These residues are clustered at one end of the beta-barrel, and their side chains are oriented toward the interior of the barrel. Cu(I) can be modeled into the apo-CusF structure with only minimal structural changes using H36, M47, and M49 as ligands. The unique structure and metal binding site of CusF are distinct from those of previously characterized copper-binding proteins.     

Ca2+-selective transient receptor potential V channel architecture and function require a specific ankyrin repeat

Transient receptor potential (TRP) proteins form cation-conducting ion channels with currently 28 known genes encoding TRP channel monomers in mammals. These monomers are thought to coassemble to form homo- or heterotetrameric channels, but the signals governing their assembly are unknown. Within the TRPV subgroup, TRPV5 and TRPV6 show exclusive calcium selectivity and play an important role in calcium uptake. To identify signals that mediate assembly of functional TRPV6, we screened domains for self-association using co-immunoprecipitation, sucrose gradient centrifugation, bacterial two-hybrid assays, and patch clamp analysis. Of the two identified interaction domains within the N-terminal region, we showed that the first domain encompassing the third ankyrin repeat is the stringent requirement for physical assembly of TRPV6 subunits and when transferred to an unrelated protein enables its interaction with TRPV6. Deletion of this repeat or mutation of critical residues within this repeat rendered nonfunctional channels that do not co-immunoprecipitate or form tetramers. Suppression of dominant-negative inhibitors of TRPV6-specific currents was achieved by deletion of ankyrin (ANK) 3. We propose that the third ANK repeat initiates a molecular zippering process that proceeds past the fifth ANK repeat and creates an intracellular anchor that is necessary for functional subunit assembly.     

Determination of the major mercapturic acids of acrylamide and glycidamide in human urine by LC-ESI-MS/MS

We developed a LC-MS/MS method for the quantitative determination of the mercapturic acid (MA) metabolites of acrylamide (AA) AAMA and of its oxidative metabolite glycidamide (GA) GAMA in urine samples from the general population. The method requires 4 mL of urine which is solid phase extracted prior to LC-MS/MS analysis. The metabolites are detected by ESI-tandem mass spectrometry in negative ionisation mode and quantified by isotope dilution. Detection limits ranged down to 1.5 microg/L urine for both AAMA and GAMA. The imprecision expressed as R.S.D. lay between 2% and 6% for both analytes (intra- and inter-assay). First results on a small group of 29 persons out of the general population ranged from 5 to 338 microg/L AAMA and 相似文献   

Experimental and structural investigations of anemochorous dispersal

The present paper describes the anemochorous dispersal of representative diaspores of Asteraceae, Dipsacaceae, and Poaceae from xerothermic grassland communities of Central and Northeastern Germany. For eleven species, potential dispersal distance was determined by fall velocity experiments as well as by taking into account the diaspore flight angle under the influence of an artificially-produced, regularly, and horizontally blowing air stream. The latter is a new and comparatively simple method enabling the implementation of mathematical formulas which describe the potential flight capacity of a diaspore for different wind speeds and exposition heights. Surface structures, shown by a scanning electron microscope, were consulted for the interpretation of results.Of the species considered, the best fliers are the diaspores of Asteraceae and Melica ciliata (Poaceae) characterized by a plumous pappus or a hairy lemma. The wing-like attachments of the diaspores of the other investigated Poaceae and Dipsacaceae are clearly less efficient for wind dispersal.The fall rates of the investigated species agree to a great extent with literature data. But a critical comparison of both methods employed shows that fall velocity as a measure of horizontal diaspore flight capacity is only suitable for low wind force < 2 m s^-1. With increasing wind force, the dispersal distance of a flying diaspore does not rise in a linear, but rather in an approximately quadratic manner. Thus, in nature, conditions of higher wind forces may be very important for the reachable dispersal distances of well-flying diaspores. This could be of particular significance for nature conservation concepts concerning the vulnerability of species towards isolation within fragmented landscapes.