全文获取类型
收费全文 | 5311篇 |
免费 | 425篇 |
国内免费 | 1篇 |
专业分类
5737篇 |
出版年
2024年 | 6篇 |
2023年 | 31篇 |
2022年 | 67篇 |
2021年 | 140篇 |
2020年 | 77篇 |
2019年 | 96篇 |
2018年 | 142篇 |
2017年 | 107篇 |
2016年 | 189篇 |
2015年 | 267篇 |
2014年 | 279篇 |
2013年 | 393篇 |
2012年 | 435篇 |
2011年 | 481篇 |
2010年 | 295篇 |
2009年 | 257篇 |
2008年 | 306篇 |
2007年 | 353篇 |
2006年 | 296篇 |
2005年 | 267篇 |
2004年 | 264篇 |
2003年 | 246篇 |
2002年 | 201篇 |
2001年 | 47篇 |
2000年 | 37篇 |
1999年 | 45篇 |
1998年 | 53篇 |
1997年 | 40篇 |
1996年 | 42篇 |
1995年 | 32篇 |
1994年 | 23篇 |
1993年 | 18篇 |
1992年 | 21篇 |
1991年 | 19篇 |
1990年 | 21篇 |
1989年 | 14篇 |
1988年 | 12篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 8篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 8篇 |
1981年 | 9篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 6篇 |
1976年 | 5篇 |
1973年 | 5篇 |
1971年 | 6篇 |
排序方式: 共有5737条查询结果,搜索用时 15 毫秒
21.
22.
Víctor Rodríguez-Prieto Deborah Kukielka Beatriz Martínez-López Ana Isabel de las Heras José Ángel Barasona Christian Gortázar José Manuel Sánchez-Vizcaíno Joaquín Vicente 《European Journal of Wildlife Research》2013,59(6):859-867
Porcine reproductive and respiratory syndrome (PRRS) is a swine infectious disease causing major economic problems on the intensive pig industry. This virus has been reported worldwide in domestic pigs and there is evidence of PRRS virus (PRRSV) infection in wild boar (Sus scrofa). Nonetheless, the epidemiological role of wild boar and extensively kept domestic pigs remains unclear. The aim of this study was to determine the occurrence of PRRS in wild boar and Iberian pigs in the dehesa ecosystem of the Castile-La Mancha region of Spain, which boasts one of the most important free-roaming porcine livestock and hunting industries in the country. Using geo-spatial analysis of literature data, we first explored the relationship between domestic pig density and PRRS occurrence in wild boar in Europe. Results revealed that PRRS occurrence in wild boar may be influenced, albeit not significantly, by domestic pig density. Next, we analyzed sera from 294 wild boar and 80 Iberian pigs by indirect enzyme-linked immunosorbent assay for PRRSV antibodies. The sera and 27 wild boar tissue samples were analyzed by two real-time RT-PCR assays, targeting the most conserved genes of the PRRSV genome, ORF1 and ORF7. Seven wild boar (2.4 %) and one Iberian pig (1.3 %) were seropositive, while none of the animals tested positive for PRRSV by RT-PCR. Our results confirm the limited spread of PRRSV in free-roaming Iberian pigs and wild boar living in mutual contact. Further studies would be necessary to address whether this low seroprevalence found in these animals reflects transmission from intensively kept pigs or the independent circulation of specific strains in free-roaming pigs. 相似文献
23.
Natália Martins Isabel C. F. R. Ferreira Lillian Barros Sónia Silva Mariana Henriques 《Mycopathologia》2014,177(5-6):223-240
Candidiasis is the most common opportunistic yeast infection. Candida species and other microorganisms are involved in this complicated fungal infection, but Candida albicans continues to be the most prevalent. In the past two decades, it has been observed an abnormal overgrowth in the gastrointestinal, urinary and respiratory tracts, not only in immunocompromised patients, but also related to nosocomial infections and even in healthy individuals. There is a widely variety of causal factors that contribute to yeast infection which means that candidiasis is a good example of a multifactorial syndrome. Due to rapid increase in the incidence in these infections, this is the subject of numerous studies. Recently, the focus of attention is the treatment and, above all, the prevention of those complications. The diagnosis of candidiasis could become quite complicated. Prevention is the most effective “treatment,” much more than eradication of the yeast with antifungal agents. There are several aspects to consider in the daily routine that can provide a strength protection. However, a therapeutic approach is necessary when the infection is established, and therefore, other alternatives should be explored. This review provides an overview on predisposition factors, prevention and diagnosis of candidiasis, highlighting alternative approaches for candidiasis treatment. 相似文献
24.
25.
26.
27.
Stefanie Wagner Isabel Sorg Matteo Degiacomi Laure Journet Matteo Dal Peraro Guy R. Cornelis 《Molecular microbiology》2009,71(3):692-701
The length of the Yersinia injectisome needle is determined by the protein YscP, which could act as a molecular ruler. The analysis of the correlation between the size of YscP and the needle length in seven wild-type strains of Yersinia enterocolitica reinforced this hypothesis but hinted that the secondary structure of YscP might influence needle length. Hence, 11 variants of YscP515 were generated by multiple Pro or Gly substitutions. The needle length changed in inverse function of the helical content, indicating that not only the number of residues but also their structure controls length. Taking the secondary motifs into account, Pro/Gly-variants were subjected to in silico modelling to simulate the extension of YscP upon needle growth. The calculated lengths when the helical content is preserved correlated strikingly with the measured needle length, with a constant difference of ∼29 nm, which corresponds approximately to the size of the basal body. These data support the ruler model and show that the functional ruler has a helical structure. 相似文献
28.
Daniela Barro‐Trastoy Esther Carrera Jorge Baos Julia Palau‐Rodríguez Omar Ruiz‐Rivero Pablo Tornero Jos M. Alonso Isabel Lpez‐Díaz María Dolores Gmez Miguel A. Prez‐Amador 《The Plant journal : for cell and molecular biology》2020,102(5):1026-1041
Ovule primordia formation is a complex developmental process with a strong impact on the production of seeds. In Arabidopsis this process is controlled by a gene network, including components of the signalling pathways of auxin, brassinosteroids (BRs) and cytokinins. Recently, we have shown that gibberellins (GAs) also play an important role in ovule primordia initiation, inhibiting ovule formation in both Arabidopsis and tomato. Here we reveal that BRs also participate in the control of ovule initiation in tomato, by promoting an increase on ovule primordia formation. Moreover, molecular and genetic analyses of the co‐regulation by GAs and BRs of the control of ovule initiation indicate that two different mechanisms occur in tomato and Arabidopsis. In tomato, GAs act downstream of BRs. BRs regulate ovule number through the downregulation of GA biosynthesis, which provokes stabilization of DELLA proteins that will finally promote ovule primordia initiation. In contrast, in Arabidopsis both GAs and BRs regulate ovule number independently of the activity levels of the other hormone. Taken together, our data strongly suggest that different molecular mechanisms could operate in different plant species to regulate identical developmental processes even, as for ovule primordia initiation, if the same set of hormones trigger similar responses, adding a new level of complexity. 相似文献
29.
Ilse Van Gucht Josephina A.N. Meester Jotte Rodrigues Bento Maaike Bastiaansen Jarl Bastianen Ilse Luyckx Lotte Van Den Heuvel Cédric H.G. Neutel Pieter-Jan Guns Mandy Vermont Erik Fransen Melanie H.A.M. Perik Joe Davis Velchev Maaike Alaerts Dorien Schepers Silke Peeters Isabel Pintelon Abdulrahman Almesned Aline Verstraeten 《American journal of human genetics》2021,108(6):1115-1125
30.