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311.
Estrogen receptor-alpha predominantly mediates the salutary effects of 17beta-estradiol on splenic macrophages following trauma-hemorrhage 总被引:1,自引:0,他引:1
Suzuki T Shimizu T Yu HP Hsieh YC Choudhry MA Bland KI Chaudry IH 《American journal of physiology. Cell physiology》2007,293(3):C978-C984
Although 17-estradiol administration following trauma-hemorrhage prevents the suppression in splenic macrophage cytokine production, it remains unknown whether the salutary effects are mediated via estrogen receptor (ER)- or ER- and which signaling pathways are involved in such 17-estradiol effects. Utilizing ER-- or ER--specific agonists, this study examined the role of ER- and ER- in 17-estradiol-mediated restoration of macrophage cytokine production following trauma-hemorrhage. In addition, since MAPK and NF-B are known to regulate macrophage cytokine production, we also examined the activation of those signaling molecules. Male rats underwent trauma-hemorrhage (mean arterial pressure of 40 mmHg for 90 min) and fluid resuscitation. The ER- agonist propyl pyrazole triol (PPT; 5 µg/kg), the ER- agonist diarylpropionitrile (DPN; 5 µg/kg), 17-estradiol (50 µg/kg), or vehicle (10% DMSO) was injected subcutaneously during resuscitation. Twenty-four hours thereafter, splenic macrophages were isolated, and their IL-6 and TNF- production and activation of MAPK and NF-B were measured. Macrophage IL-6 and TNF- production and MAPK activation were decreased, whereas NF-B activity was increased, following trauma-hemorrhage. PPT or 17-estradiol administration after trauma-hemorrhage normalized those parameters. DPN administration, on the other hand, did not normalize the above parameters. Since PPT but not DPN administration following trauma-hemorrhage was as effective as 17-estradiol in preventing the suppression in macrophage cytokine production, it appears that ER- plays the predominant role in mediating the salutary effects of 17-estradiol on macrophage cytokine production following trauma-hemorrhage and that such effects are likely mediated via normalization of MAPK but not NF-B signaling pathways. shock; mitogen-activated protein kinase; nuclear factor-B; propyl pyrazole triol; diarylpropionitrile 相似文献
312.
Phylogenetic Relationships of Cryptosporidium Parasites Based on the 70-Kilodalton Heat Shock Protein (HSP70) Gene 总被引:1,自引:0,他引:1 下载免费PDF全文
Irshad M. Sulaiman Una M. Morgan R. C. Andrew Thompson Altaf A. Lal Lihua Xiao 《Applied microbiology》2000,66(6):2385-2391
We have characterized the nucleotide sequences of the 70-kDa heat shock protein (HSP70) genes of Cryptosporidium baileyi, C. felis, C. meleagridis, C. muris, C. serpentis, C. wrairi, and C. parvum from various animals. Results of the phylogenetic analysis revealed the presence of several genetically distinct species in the genus Cryptosporidium and eight distinct genotypes within the species C. parvum. Some of the latter may represent cryptic species. The phylogenetic tree constructed from these sequences is in agreement with our previous results based on the small-subunit rRNA genes of Cryptosporidium parasites. The Cryptosporidium species formed two major clades: isolates of C. muris and C. serpentis formed the first major group, while isolates of C. felis, C. meleagridis, C. wrairi, and eight genotypes of C. parvum formed the second major group. Sequence variations were also observed between C. muris isolates from ruminants and rodents. The HSP70 gene provides another useful locus for phylogenetic analysis of the genus Cryptosporidium. 相似文献
313.
Sequence Differences in the Diagnostic Target Region of the Oocyst Wall Protein Gene of Cryptosporidium Parasites 总被引:2,自引:0,他引:2 下载免费PDF全文
Lihua Xiao Josef Limor Una M. Morgan Irshad M. Sulaiman R. C. Andrew Thompson Altaf A. Lal 《Applied microbiology》2000,66(12):5499-5502
Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp. and genotypes. 相似文献
314.
Eggs of the silver pomfret,Pampus argenteus, were collected and artificially fertilized by stripping fully-ripe male and female broodstock caught by gillnets in Kuwait
waters during June 1997. Larvae hatched from fertilized eggs were reared until 90 days after hatching (DAH) in water temperatures
of 27–30°C. Newly-hatched larvae grew from an average of 2.4 mm in body length (BL) to 3.7, 4.4, 7.2 and 8.4 mm at 8, 12,
24 and 30 DAH, respectively. Myomere and vertebral numbers ranged from 34 to 36. Transformation from the larval to juvenile
form was completed at 22.2 mm BL (40 DAH). Dorsal and anal fin spines first appeared when juveniles reached 38.8 mm BL (50
DAH). Body depth increased with increase in body length; a rapid increase in body depth occurred in larvae 7.1–8.0 mm, reaching
57% of BL, and further increased to 69% of BL in juveniles 38.8 to 47.9 mm. Pigmentation during development is described and
illustrated. 相似文献
315.
Erejuwa OO Sulaiman SA Wahab MS Sirajudeen KN Salleh MS Gurtu S 《International journal of biological sciences》2011,7(2):244-252
Diabetes mellitus is associated with deterioration of glycemic control and progressive metabolic derangements. This study investigated the effect of honey as an adjunct to glibenclamide or metformin on glycemic control in streptozotocin-induced diabetic rats. Diabetes was induced in rats by streptozotocin. The diabetic rats were randomized into six groups and administered distilled water, honey, glibenclamide, glibenclamide and honey, metformin or metformin and honey. The animals were treated orally once daily for four weeks. The diabetic control rats showed hypoinsulinemia (0.27 ± 0.01 ng/ml), hyperglycemia (22.4 ± 1.0 mmol/L) and increased fructosamine (360.0 ± 15.6 μmol/L). Honey significantly increased insulin (0.41 ± 0.06 ng/ml), decreased hyperglycemia (12.3 ± 3.1 mmol/L) and fructosamine (304.5 ± 10.1 μmol/L). Although glibenclamide or metformin alone significantly (p < 0.05) reduced hyperglycemia, glibenclamide or metformin combined with honey produced significantly much lower blood glucose (8.8 ± 2.9 or 9.9 ± 3.3 mmol/L, respectively) compared to glibenclamide or metformin alone (13.9 ± 3.4 or 13.2 ± 2.9 mmol/L, respectively). Similarly, glibenclamide or metformin combined with honey produced significantly (p < 0.05) lower fructosamine levels (301.3 ± 19.5 or 285.8 ± 22.6 μmol/L, respectively) whereas glibenclamide or metformin alone did not decrease fructosamine (330.0 ± 29.9 or 314.6 ± 17.9 μmol/L, respectively). Besides, these drugs or their combination with honey increased insulin levels. Glibenclamide or metformin combined with honey also significantly reduced the elevated levels of creatinine, bilirubin, triglycerides, and VLDL cholesterol. These results indicate that combination of glibenclamide or metformin with honey improves glycemic control, and provides additional metabolic benefits, not achieved with either glibenclamide or metformin alone. 相似文献
316.
The yeast Candida tropicalis produces xylitol, a natural, low-calorie sweetener whose metabolism does not require insulin, by catalytic activity of NADPH-dependent xylose reductase. The oxidative pentose phosphate pathway (PPP) is a major basis for NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes which, respectively encodes glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "PP", than in BSXDH-3. In fermentation experiments using glycerol as a co-substrate with xylose, strain PP showed volumetric xylitol productivity of 1.25 g l(-1) h(-1), 21% higher than the rate (1.04 g l(-1) h(-1)) in BSXDH-3. This is the first report of increased metabolic flux toward PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production. 相似文献
317.
通过实地取样、标本查阅和文献研究获得131个锁阳(Cynomorium songaricum)采样点数据,并运用ArcGIS技术平台,从气候、土壤、地形数据库中提取了各采样点的生态因子,得出锁阳适宜生态因子范围。以此为据,利用中药材产地适宜性分析系统(TCMGIS)对锁阳在中国的产地适宜性进行多因子的空间分析。结果表明,锁阳适生区域主要位于西北干旱地区,包括内蒙古、新疆、宁夏、甘肃、青海等省(区)。研究结果对我国西北地区防沙固沙、荒漠生态保护和恢复具有重要的社会效应和生态价值。 相似文献
318.
Tariq Ahmad Masoodi Sulaiman A. Al Shammari May N. Al-Muammar Adel A. Alhamdan Venkateswar Rao Talluri 《Gene》2013
Non-synonymous single nucleotide polymorphisms (nsSNPs) are considered as biomarkers to disease susceptibility. In the present study, nsSNPs in CLU, PICALM and BIN1 genes were screened for their functional impact on concerned proteins and their plausible role in Alzheimer disease (AD) susceptibility. Initially, SNPs were retrieved from dbSNP database, followed by identification of potentially deleterious nsSNPs and prediction of their effect on proteins by PolyPhen and SIFT. Protein stability and the probability of mutation occurrence were predicted using I-Mutant and PANTHER respectively. SNPs3D and FASTSNP were used for the functional analysis of nsSNPs. The functional impact on the 3D structure of proteins was evaluated by SWISSPDB viewer and NOMAD-Ref server. On analysis, 3 nsSNPs with IDs rs12800974 (T158P) of PICALM and rs11554585 (R397C) and rs11554585 (N106D) of BIN1 were predicted to be functionally significant with higher scores of I-Mutant, SIFT, PolyPhen, PANTHER, FASTSNP and SNPs3D. The mutant models of these nsSNPs also showed very high energies and RMSD values compared to their native structures. Current study proposes that the three nsSNPs identified in this study constitute a unique resource of potential genetic factors for AD susceptibility. 相似文献
319.
Haleema Shahin D. H. Rokeya Sultana Juveriya Farooq Tahreen Taj Umaima Farheen Khaiser Nader Sulaiman Ayyt Alanazi Mohammed Kanan Alshammari Mohammad Nazal Alshammari Firas Hamdan Alsubaie Syed Mohammed Basheeruddin Asdaq Abdulmueen A. Alotaibi Abdulrhman ahmed Alamir Mohd. Imran Shahamah Jomah 《Current issues in molecular biology》2022,44(7):2887
Diabetic nephropathy (DN) is a serious kidney illness characterized by proteinuria, glomerular enlargement, reduced glomerular filtration, and renal fibrosis. DN is the most common cause of end-stage kidney disease, accounting for nearly one-third of all cases of diabetes worldwide. Hyperglycemia is a major factor in the onset and progression of diabetic nephropathy. Many contemporary medicines are derived from plants since they have therapeutic properties and are relatively free of adverse effects. Glycosides, alkaloids, terpenoids, and flavonoids are among the few chemical compounds found in plants that are utilized to treat diabetic nephropathy. The purpose of this review was to consolidate information on the clinical and pharmacological evidence supporting the use of a variety of medicinal plants to treat diabetic nephropathy. 相似文献
320.
Ghulam Mustafa Asia Iqbal Arshad Javid Maleeha Manzoor Sumaira Aslam Ahmad Ali Sheikh Muhammad Azam Muhammad Khalid Muhammad Farooq Yahya Al Naggar Sulaiman Ali Alharbi Hesham Ali El Enshasy Roslinda Abd Malek Samina Qamer Ali Hussain 《Saudi Journal of Biological Sciences》2022,29(2):730-734
Now-a-days, different bioproducts are being used extensively for the welfare of mankind. However, for proper utility of any bioproduct, the exact biotechnological potential of that product should be explored. Honey is produced in almost every country on the planet. It has long been used as a medicinal agent in addition to its broader use as a popular food throughout the human history. It can be used to treat various diseases without causing any negative side effects. In the present study, the antibacterial potential of honey produced by A. dorsata was investigated at its variable concentrations (25, 50, 75 and 100 %) against four pathogenic bacterial species. The highest antimicrobial action was seen against E. coli at 100 % concentration of the honey while showing zone of inhibition of 37.5 ± 3.5 mm. However, the lowest antibacterial action was observed against E. faecalis. The overall order of growth inhibition by the honey at its 100 % concentration for the implicated bacterial species appeared as: E. coli ˃ P. aeruginosa ˃ S. aureus ˃ E. faecalis. The honey couldn’t show antibacterial action at its 25 % concentration. Our findings of the present study will be helpful for utility of the honey as an alternative medicine for curing different complications caused by microbial pathogens. 相似文献