全文获取类型
收费全文 | 5892篇 |
免费 | 436篇 |
国内免费 | 4篇 |
出版年
2023年 | 37篇 |
2022年 | 73篇 |
2021年 | 148篇 |
2020年 | 91篇 |
2019年 | 109篇 |
2018年 | 200篇 |
2017年 | 142篇 |
2016年 | 240篇 |
2015年 | 329篇 |
2014年 | 370篇 |
2013年 | 461篇 |
2012年 | 545篇 |
2011年 | 480篇 |
2010年 | 318篇 |
2009年 | 241篇 |
2008年 | 359篇 |
2007年 | 293篇 |
2006年 | 271篇 |
2005年 | 246篇 |
2004年 | 221篇 |
2003年 | 204篇 |
2002年 | 192篇 |
2001年 | 56篇 |
2000年 | 51篇 |
1999年 | 51篇 |
1998年 | 59篇 |
1997年 | 17篇 |
1996年 | 33篇 |
1995年 | 21篇 |
1994年 | 22篇 |
1993年 | 20篇 |
1992年 | 21篇 |
1991年 | 18篇 |
1990年 | 24篇 |
1989年 | 22篇 |
1988年 | 17篇 |
1987年 | 18篇 |
1986年 | 12篇 |
1985年 | 12篇 |
1984年 | 28篇 |
1983年 | 18篇 |
1982年 | 18篇 |
1981年 | 19篇 |
1980年 | 20篇 |
1979年 | 18篇 |
1978年 | 17篇 |
1977年 | 9篇 |
1973年 | 10篇 |
1964年 | 8篇 |
1962年 | 8篇 |
排序方式: 共有6332条查询结果,搜索用时 15 毫秒
941.
Interstitial cells of Cajal: mediators of communication between circular and longitudinal muscle layers of canine colon 总被引:3,自引:0,他引:3
Louis W. C. Liu Laura Farraway Irene Berezin J. D. Huizinga 《Cell and tissue research》1998,294(1):69-79
The network of interstitial cells of Cajal associated with Auerbach’s (myenteric) plexus in the canine colon was investigated
to determine its role in facilitating communication between circular and longitudinal muscle layers. Electrical coupling between
the muscle layers was demonstrated by propagating extracellularly evoked electrotonic pulses from circular muscle cells to
nearby longitudinal muscle cells. The likelihood of cytoplasmic continuity across Auerbach’s plexus was further demonstrated
by the ability of neurobiotin to spread between the interstitial cells and the circular and longitudinal muscle cells. Importantly,
direct neurobiotin spread between circular and longitudinal muscle cells was not observed even when they were in close proximity
as determined by confocal microscopy. When neurobiotin did spread across the two muscle layers, the intervening interstitial
cells were always neurobiotin-positive. In regions where circular and longitudinal muscle cells approach each other closely,
electron microscopy revealed the presence of close appositions between interstitial cells and smooth muscle cells. Gap junctions
between interstitial cells and smooth muscle cells of both layers, as judged by electron microscopy, were extremely rare.
Neither gap junctions nor close appositions were observed between longitudinal and circular muscle cells. The special arrangement
for electrotonic coupling across Auerbach’s plexus through interstitial cells of Cajal suggests controlled coupling between
the two muscle layers, explaining the preservation of their distinct electrical activities.
Received: 21 July 1995 / Accepted: 22 April 1998 相似文献
942.
943.
A possible role of adenylate cyclase in the longterm dietary regulation of insulin secretion from rat islets of Langerhans 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
1. Adenylate cyclase activity and patterns of insulin release in response to various concentrations of glucose were determined in islets of Langerhans isolated from starving, fed, or glucose-loaded rats. 2. Basal and glucagon-stimulated activities of adenylate cyclase were lower in islets from starved than from fed rats. The minimum glucose concentration required for stimulation of insulin secretion was higher, whereas the maximum secretory response to glucose was lower, in islets from starved than from fed rats. 3. Adenylate cyclase activity in islets of Langerhans obtained from fed rats loaded with glucose by intermittent intravenous or intraperitoneal injections over 5h was significantly higher than that seen in islets from normal fed rats. Islets obtained from glucose-loaded rats required a lower glucose concentration for stimulation of insulin secretion and attained a higher maximal response to glucose stimulation than those derived from fed rats. 4. Incubation in vitro of islets isolated from normal fed rats, for periods of 1 to 24h in the presence of high concentrations of glucose resulted in an activation of adenylate cyclase that occurred progressively from 2 to 7h and which was maintained during 24h of incubation. The increase of adenylate cyclase activity in isolated islets incubated for 4h in the presence of glucose was not prevented by addition of cycloheximide or actinomycin D. Galactose or 2-deoxyglucose was ineffective in increasing adenylate cyclase activity, and pyruvate (20mm) was less effective than glucose. 5. It is suggested that glucose or a glucose metabolite may exert long-term effects on islet cell adenylate cyclase. 相似文献
944.
945.
946.
Francisco Gerson Araújo Tatiana Pires Teixeira Ana Paula Penha Guedes Márcia Cristina Costa de Azevedo André Luiz Machado Pessanha 《Hydrobiologia》2018,817(1):205-213
Myriophyllum aquaticum is a semi-submerged exotic macrophyte that was introduced to China for many years. This species may be found in an emergent form in aquatic environments or in an amphibious form under drained conditions. Nuisance growth of this species has often been attributed to excessive amounts of nutrients. Therefore, we tested the following hypotheses: (1) high nutrient availability facilitates the establishment of M. aquaticum and (2) fragment type interacts with nutrient availability to determine the colonization and regeneration capacities of M. aquaticum. Two types of fragments were grown in water solutions with two levels of phosphorous. After 3 weeks, the survival rates showed no significant difference between the phosphorous treatments. However, emergent fragments showed higher RGR in the low and high phosphorous treatments than amphibious fragments. In addition, emergent fragments also showed higher regeneration capacities, indicating higher invasiveness in emergent fragments compared to amphibious fragments. Moreover, the high phosphorous concentration caused emergent fragments to produce more branches, indicating that nutrient availability may increase M. aquaticum propagule pressure. Our study highlights that nutrient supply increased emergent fragment establishment and shaped the invasion dynamics of macrophytes, which could help predict the spread and potential impact of exotic macrophytes in natural aquatic ecosystems. 相似文献
947.
Inter‐annual variability of fruit timing and quantity at Nouragues (French Guiana): insights from hierarchical Bayesian analyses
下载免费PDF全文
![点击此处可从《Biotropica》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Irene Mendoza Richard S. Condit S. Joseph Wright Adeline Caubère Patrick Châtelet Isabelle Hardy Pierre‐Michel Forget 《Biotropica》2018,50(3):431-441
The timing and quantity of fruit production are major determinants of the functioning of a forest community, but simultaneous analyses of both are rare. We analyzed a ten‐year dataset (2001–2011) of fruit production for 45 tree and liana species from the Nouragues rain forest, French Guiana. We developed a hierarchical Bayesian approach to determine variation in the timing and quantity of fruit production. Our analysis accommodates missing censuses and quantifies variation at seasonal and inter‐annual scales. The fruiting peak of 22 of 45 species occurred during the peak of the rainy season, which is typical for central and eastern Amazon. The timing and quantity of fruit production varied substantially across years in most species, with greater variation in quantity than in timing. The timing of fruit production varied from continuously fruiting species to mast fruiting species that had two or more consecutive years without fruit production. Fully 40% of species were mast fruiting species. The seasonal timing and inter‐annual variation in fruiting were unrelated to seed dispersal mode across species. We saw no evidence for directional change in the level of fruit production, the timing of fruit production, or their variances; however, 10 yr is a short record for such analyses. 相似文献
948.
Stefan Schindler Barbara Livoreil Isabel Sousa Pinto Rita M. Araújo Klaus Peter Zulka Andrew S. Pullin Luis Santamaria Michaela Kropik Pablo Fernández-Méndez Thomas Wrbka 《Biodiversity and Conservation》2016,25(7):1301-1318
In order to develop BiodiversityKnowledge, a Network of Knowledge working at the European science–policy interface for biodiversity and ecosystem services, we conducted three trial assessments. Their purpose was to test structure and processes of the knowledge synthesis function and to produce knowledge syntheses. The trial assessments covered conservation and management of kelp ecosystems, biological control of agricultural pests, and conservation and multifunctional management of floodplains. Following the BiodiversityKnowledge processes, we set up expert consultations, systematic reviews, and collaborative adaptive management procedures in collaboration with requesters, policy and decision-makers, stakeholders, and knowledge holders. Outputs included expert consultations, systematic review protocols, a group model and a policy brief. Important lessons learned were firstly that the scoping process, in which requesters and experts iteratively negotiate the scope, scale and synthesis methodology, is of paramount importance to maximize the scientific credibility and policy relevance of the output. Secondly, selection of a broad array of experts with diverse and complementary skills (including multidisciplinary background and a broad geographical coverage) and participation of all relevant stakeholders is crucial to ensure an adequate breath of expertise, better methodological choices, and maximal uptake of outcomes: Thirdly, as the most important challenge was expert and stakeholder engagement, a high visibility and reputation of BiodiversityKnowledge, supported by an incentive system for participation, will be crucial to ensure such engagement. We conclude that BiodiversityKnowledge has potential for a good performance in delivering assessments, but it requires adequate funding, trust-building among knowledge holders and stakeholders, and a proactive and robust interface with the policy and decision making community. 相似文献
949.
The active microbial diversity drives ecosystem multifunctionality and is physiologically related to carbon availability in Mediterranean semi‐arid soils
下载免费PDF全文
![点击此处可从《Molecular ecology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Felipe Bastida Irene F. Torres José L. Moreno Petr Baldrian Sara Ondoño Antonio Ruiz‐Navarro Teresa Hernández Hans H. Richnow Robert Starke Carlos García Nico Jehmlich 《Molecular ecology》2016,25(18):4660-4673
Biogeochemical processes and ecosystemic functions are mostly driven by soil microbial communities. However, most methods focus on evaluating the total microbial community and fail to discriminate its active fraction which is linked to soil functionality. Precisely, the activity of the microbial community is strongly limited by the availability of organic carbon (C) in soils under arid and semi‐arid climate. Here, we provide a complementary genomic and metaproteomic approach to investigate the relationships between the diversity of the total community, the active diversity and ecosystem functionality across a dissolved organic carbon (DOC) gradient in southeast Spain. DOC correlated with the ecosystem multifunctionality index composed by soil respiration, enzyme activities (urease, alkaline phosphatase and β‐glucosidase) and microbial biomass (phospholipid fatty acids, PLFA). This study highlights that the active diversity (determined by metaprotoemics) but not the diversity of the whole microbial community (evaluated by amplicon gene sequencing) is related to the availability of organic C and it is also connected to the ecosystem multifunctionality index. We reveal that DOC shapes the activities of bacterial and fungal populations in Mediterranean semi‐arid soils and determines the compartmentalization of functional niches. For instance, Rhizobales thrived at high‐DOC sites probably fuelled by metabolism of one‐C compounds. Moreover, the analysis of proteins involved in the transport and metabolism of carbohydrates revealed that Ascomycota and Basidiomycota occupied different nutritional niches. The functional mechanisms for niche specialization were not constant across the DOC gradient. 相似文献
950.
Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis
下载免费PDF全文
![点击此处可从《Microbiology and immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
María M. Bigi Federico Carlos Blanco Flabio R. Araújo Tyler C. Thacker Martín J. Zumárraga Angel A. Cataldi Marcelo A. Soria Fabiana Bigi 《Microbiology and immunology》2016,60(8):552-560
Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTC evolved. The genome of M. bovis is over >99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In the present study, we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non‐synonymous mutations in 202 coding sequences of regulatory genes between both species. We identified species‐specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia‐related genes between M. bovis and M. tuberculosis. 相似文献