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排序方式: 共有226条查询结果,搜索用时 62 毫秒
71.
72.
Carlo Rinaldi Christopher Grunseich Irina F. Sevrioukova Alice Schindler Iren Horkayne-Szakaly Costanza Lamperti Guida Landouré Marina L. Kennerson Barrington G. Burnett Carsten Bönnemann Leslie G. Biesecker Daniele Ghezzi Massimo Zeviani Kenneth H. Fischbeck 《American journal of human genetics》2012
73.
74.
Chhuneja P Kaur S Garg T Ghai M Kaur S Prashar M Bains NS Goel RK Keller B Dhaliwal HS Singh K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(3):313-324
Stripe rust, caused by Puccinia striiformis West. f.sp. tritici, is one of the most damaging diseases of wheat worldwide. Forty genes for stripe rust resistance have been catalogued so
far, but the majority of them are not effective against emerging pathotypes. Triticum monococcum and T. boeoticum have excellent levels of resistance to rusts, but so far, no stripe rust resistance gene has been identified or transferred
from these species. A set of 121 RILs generated from a cross involving T. monococcum (acc. pau14087) and T. boeoticum (acc. pau5088) was screened for 3 years against a mixture of pathotypes under field conditions. The parental accessions were
susceptible to all the prevalent pathotypes at the seedling stage, but resistant at the adult plant stage. Genetic analysis
of the RIL population revealed the presence of two genes for stripe rust resistance, with one gene each being contributed
by each of the parental lines. A linkage map with 169 SSR and RFLP loci generated from a set of 93 RILs was used for mapping
these resistance genes. Based on phenotypic data for 3 years and the pooled data, two QTLs, one each in T. monococcum acc. pau14087 and T. boeoticum acc. pau5088, were detected for resistance in the RIL population. The QTL in T. monococcum mapped on chromosome 2A in a 3.6 cM interval between Xwmc407 and Xwmc170, whereas the QTL from T. boeoticum mapped on 5A in 8.9 cM interval between Xbarc151 and Xcfd12 and these were designated as QYrtm.pau-2A and QYrtb.pau-5A, respectively. Based on field data for 3 years, their R
2 values were 14 and 24%, respectively. T. monococcum acc. pau14087 and three resistant RILs were crossed to hexaploid wheat cvs WL711 and PBW343, using T. durum as a bridging species with the objective of transferring these genes into hexaploid wheat. The B genome of T. durum suppressed resistance in the F1 plants, but with subsequent backcrossing one resistance gene could be transferred from one of the RILs to the hexaploid wheat
background. This gene was derived from T. boeoticum acc. pau5088 as indicated by co-introgression of T. boeoticum sequences linked to stripe rust resistance QTL, QYrtb.pau-5A. Homozygous resistant progenies with 40–42 chromosomes have been identified.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
75.
76.
A novel method for nucleic acid sequence determination 总被引:30,自引:0,他引:30
We describe a novel sequencing methodology which should be readily and completely automated. The method relies on fragmentation of a nucleotide or deoxynucleotide sequence into short fragments, and subsequent quantitation of the fragments by hybridization to oligo-deoxynucleotides on a solid support. The original sequence may be reconstructed from the resulting table of fragment frequencies. We present a specific protocol which would allow practical implementation of this approach. 相似文献
77.
Rate of base substitution in mammalian nuclear DNA is dependent on local sequence context 总被引:1,自引:0,他引:1
We have analysed the pattern of base substitutions in 40 mammalian processed pseudogenes by comparing them with their cognate functional genes. We find that the rate of base substitution is dependent both on the nature of the mutating base and upon the bases which flank it. Among bases which are not members of CG dinucleotides, bases which are the 5' members of purine-pyrimidine or pyrimidine-purine dinucleotides mutate faster than 5' members of other dinucleotides. These data allow the mutational distance between genes to be estimated more accurately, and suggest some general properties of the mechanism of error prevention in mammalian nuclear DNA. 相似文献
78.
In addition to exhibiting swimming and twitching motility, Pseudomonas aeruginosa is able to swarm on semisolid (viscous) surfaces. Recent studies have indicated that swarming is a more complex type of motility influenced by a large number of different genes. To investigate the adaptation process involved in swarming motility, gene expression profiles were analyzed by performing microarrays on bacteria from the leading edge of a swarm zone compared to bacteria growing in identical medium under swimming conditions. Major shifts in gene expression patterns were observed under swarming conditions, including, among others, the overexpression of a large number of virulence-related genes such as those encoding the type III secretion system and its effectors, those encoding extracellular proteases, and those associated with iron transport. In addition, swarming cells exhibited adaptive antibiotic resistance against polymyxin B, gentamicin, and ciprofloxacin compared to what was seen for their planktonic (swimming) counterparts. By analyzing a large subset of up-regulated genes, we were able to show that two virulence genes, lasB and pvdQ, were required for swarming motility. These results clearly favored the conclusion that swarming of P. aeruginosa is a complex adaptation process in response to a viscous environment resulting in a substantial change in virulence gene expression and antibiotic resistance. 相似文献
79.
Frank Van Iren M. Lonlou Joolen Anton F. C. Gerritsen Marcel A. W. Noordervliet F. Pinie Boers-Van der Sluijs 《Physiologia plantarum》1981,52(1):15-22
In roots of low-salt barley ( Hordeum vulgare L.), translocation of K+ , as well as of several other ions, from the root to the shoot starts after a lag-time. Its length is independent of external concentration. The onset of upward translocation can be remarkably abrupt. Three hypotheses that could explain the lag-phase were tested:
All three hypotheses are rejected. Within the whole root radial as well as longitudinal equilibration appears to be very rapid. The simple model of compartmentation is rejected. A more complex compartmentation of the cytoplasm (symplasm) in which the endopiasmic reticulum could play a predominant role is suggested. The mechanisms of vacuolar accumulation and upward translocation appear biochemically related. 相似文献
- 1.
It is the result of successive loading of the concentric root cell layers centripetally from the epidermis.
- 2.
It is the result of successive loading of the root basipetally from the apex.
- 3.
It is the result of induction of the mechanism(s) responsible for upward translocation.
All three hypotheses are rejected. Within the whole root radial as well as longitudinal equilibration appears to be very rapid. The simple model of compartmentation is rejected. A more complex compartmentation of the cytoplasm (symplasm) in which the endopiasmic reticulum could play a predominant role is suggested. The mechanisms of vacuolar accumulation and upward translocation appear biochemically related. 相似文献
80.
E. G. M. Meijer F. van Iren E. Schrijnemakers L. A. M. Hensgens M. van Zijderveld R. A. Schilperoort 《Plant cell reports》1991,10(4):171-174
A method is described for cryopreservation of cell suspension lines of rice (Oryza sativa L.) for use in protoplast research and as a way of retaining desirable characteristics of cell lines. The procedure involves pre-culture with mannitol, addition of a cryoprotectant solution of sucrose, dimethyl sulfoxide, glycerol and L-proline, two step freezing and storage in liquid nitrogen. Cells have been preserved for up to 14 months (the longest period tried in these experiments). Cryopreserved cells proliferated after plating on solid medium and new cell suspensions could be initiated within 15 days. Viable protoplasts, capable of divisions and callus formation, could be obtained 15–21 days after thawing. Variation between cell lines in terms of recovery rate after cryopreservation occurred. Differences between cell lines in plating efficiencies on solidified medium, however, contributed to this variation. Protoplasts from cryopreserved regenerable cell lines gave rise to embryogenic callus from which plants could be regenerated. These plants developed to maturity. A transformed cell line was also cryopreserved and it had retained the hygromycin resistance and regenerative capacity of the original cell line.Abbreviations DMSO
dimethyl sulfoxide
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
1-naphtylacetic acid
- FDA
fluorescein diacetate 相似文献