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排序方式: 共有229条查询结果,搜索用时 15 毫秒
101.
102.
Maryam Borhani-Haghighi Yousef Mohamadi Iraj Ragerdi Kashani 《Journal of cellular biochemistry》2019,120(8):12785-12795
Prenatal white matter injury is a serious problem due to maternal inflammation leading to postnatal disabilities. In this study, we used the periventricular leukomalacia (PVL) model as a common prenatal white matter injury by maternal administration of lipopolysaccharide (LPS). Neural stem cells (NSCs) have shown therapeutic ability in neurological disorders through a different mechanism such as immunomodulation. Here, we studied the preventive potential of NSCs following in utero transplantation into the embryonic lateral ventricle in an LPS-induced white matter injury model. Pregnant animals were divided into three groups and received phosphate buffered saline, LPS, or LPS + NSCs. The brains of offspring were obtained and evaluated by real-time polymerase chain reaction (PCR), immunohistochemy, enzyme-linked immunosorbent assay (ELISA), terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling (TUNEL), and caspase-3 activity assay. The LPS-induced maternal inflammation degenerated the myelin sheath in the offspring periventricular region which was associated with an increased microglial number, oligodendrocytes degeneration, proinflammatory cytokine secretion, and cell apoptosis. The transplanted NSCs homed into the brain and ameliorated the evaluated parameters. The expression of proinflammatory cytokines interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), cell apoptosis and caspase-3 activity were inhibited by NSCs. In addition, Olig2 and myelin basic protein immunohistochemy staining showed that prenatal NSCs transplantation augmented the myelination in the periventricular white matter of offspring. In conclusion, we think that prenatal therapeutic strategies, such as in utero NSCs transplantation, may prevent prenatal white matter injury after birth. 相似文献
103.
Heidar Toolee Tayebeh Rastegar Somayeh Solhjoo Keywan Mortezaee Mahshid Mohammadipour Iraj Regerdi Kashani Mohammad Akbari 《Journal of cellular biochemistry》2019,120(4):5042-5054
Kisspeptin (Kp) expression in testis has caused most of the recent research surveying its functional role in this organ. This peptide influences spermatogenesis and sperm capacitation, so it is considered as a regulator of reproduction. Kp roles exert through hypothalamic/pituitary/gonadal axis. We aimed to evaluate direct roles for Kp on proliferation and differentiation of spermatogonial cells (SCs) when the cells are cocultured with somatic cells. Somatic cells and SCs were isolated from adult azoospermic and newborn mice and then enriched using a differential attachment technique. After the evaluation of identity and colonization for SCs, the cells were cocultured with somatic cells, and three doses of Kp (10−8-10−6 M) was assessed on proliferation (through evaluation of MVH and ID4 markers) and differentiation (via evaluation of c-Kit and SCP3, TP1, TP2, and, Prm1 markers) of the coculture system. Investigations were continued for four succeeding weeks. At the end of each level of testosterone in the culture media was also evaluated. We found positive influence from Kp on proliferative and differentiative markers in SCs cocultured with somatic cells. These effects were dose-dependent. There was no effect for Kp on testosterone level. From our findings, we simply conclude that Kp as a neuropeptide for influencing central part of reproductive axis could also positively affect peripheral processes related to spermatogenesis without having an effect on steroidogenesis. 相似文献
104.
Fatemeh Araste Walead Ebrahimizadeh Iraj Rasooli Masoumeh Rajabibazl Seyed Latif Mousavi Gargari 《Biotechnology letters》2014,36(1):21-28
Expression of carbonic anhydrase IX (CAIX) significantly increases under hypoxic conditions in tumor cells. CAIX activity is executed by the catalytic domain (CA) located on the extracellular part of the enzyme. Neutralization of CAIX enzymatic activity reduces malignancy and survival of tumor cells. To inhibit the enzymatic activity, a VHH nanobody was developed against the CA domain of CAIX using phage display technology. Following immunization of a camel with the recombinant CAIX, VHH fragments were isolated by nested PCR on lymphocyte cDNA. Binding affinity of isolated nanobodies was tested by ELISA. A clone (K24) with the highest binding affinity was expressed in a soluble form. Affinity of K24 nanobody was determined to be approx. 2.3 × 10?5. K24 nanobody recognized the expressed CAIX in the HeLa cell lines with high selectivity and specificity. These findings thus have usefulness for the diagnosis and treatment of cancers. 相似文献
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Tanja Miloti Christophe Baltzinger Carsten Eichberg Amy E. Eycott Marco Heurich Jrg Müller Jorge A. Noriega Rosa Menendez Jutta Stadler Rka dm Tessa Bargmann Isabelle Bilger Jrn Buse Joaquín Calatayud Constantin Ciubuc Gergely Boros Pierre Jay‐Robert Mrt Kruus Enno Merivee Geoffrey Miessen Anne Must Elham Ardali Elena Preda Iraj Rahimi Dirk Rohwedder Rob Rose Eleanor M. Slade Lszl Somay Pejman Tahmasebi Stefano Ziani Maurice Hoffmann 《Journal of Biogeography》2019,46(1):70-82
107.
Effect of Zinc Methionine or Zinc Sulfate Supplementation on Milk Production and Composition of Milk in Lactating Dairy Cows 总被引:1,自引:0,他引:1
Saeid Sobhanirad Dorthe Carlson Reza Bahari Kashani 《Biological trace element research》2010,136(1):48-54
Eighteen lactating dairy cows were used to compare the effects of organic and inorganic Zn supplements on milk production
and chemical composition of milk. Animals received three diets in a randomized block design: basal diet with no supplemental
Zn (control, 42 mg Zn/kg), basal diet plus 500 mg Zn/kg of dry matter (DM) as zinc sulfate monohydrate (ZnS) and basal diet
plus 500 mg Zn/kg of DM as zinc methionine (ZnM). Results showed that milk and fat-corrected milk yield in dairy cows were
not significantly affected by Zn source although a numerical increase was observed. The percentages of protein, lactose, fat,
solid nonfat, total solid, and density of milk were not significantly different between treatments. However, dairy cows that
received ZnM tended to produce more milk and fat-corrected milk with a lower somatic cell count as compared to controls. The
zinc concentration in milk in the ZnM and ZnS groups was higher (P < 0.05) than in milk from the control group, but there were no significant differences between ZnS and ZnM groups. 相似文献
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109.
Objective The glutathione S-transferases (GSTs) are a superfamily of proteins that participates in detoxification. The GSTs were dividing into several
classes including omega (GSTO), mu (GSTM) and theta (GSTT) classes. In human GSTO2, GSTM1, and GSTT1 are polymorphic. In order to study whether GSTO2, GSTM1, and GSTT1 polymorphisms are associated with increased gastric cancer risk in Iranian patients, the present case–control study was done.
Methods Genomic DNA was extracted from peripheral blood of 67 gastric cancer patients and 134 control subjects. The genotyping was
performed using PCR-based method. The possible association of gastric cancer with the GSTO2 N142D polymorphism was estimated with assuming additive, dominant, and recessive effect of the variant 142D allele. To investigate whether profiles of GST genotypes are associated with the risk of gastric cancer, we used unconditional
logistic regression analysis. Results The GSTO2 142D allele in additive, dominant and recessive models was not associated with the risk. Because GSTM1, GSTT1, and GSTO2 genes belong to low-penetrance genes which might be involved in the carcinogenesis, patients and controls without family
of cancer in first-degree relatives were also analyzes separately. To investigate whether profiles of GST genotypes are associated
with the risk of gastric cancer, we used unconditional logistic regression analysis with GSTM1, GSTT1, and GSTO2 genotypes as predictor factors. The GSTO2 DD genotype was associated with decreased risk as compared to GSTO2 NN genotype (OR = 0.21, 95% CI: 0.05–0.92, P = 0.038). Conclusions Present findings show that GSTO2 DD genotype decreases the risk of gastric cancer in individuals without history of cancer in their first-degree relatives. 相似文献
110.