全文获取类型
收费全文 | 343篇 |
免费 | 14篇 |
国内免费 | 4篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 25篇 |
2021年 | 27篇 |
2020年 | 10篇 |
2019年 | 11篇 |
2018年 | 10篇 |
2017年 | 7篇 |
2016年 | 16篇 |
2015年 | 30篇 |
2014年 | 28篇 |
2013年 | 12篇 |
2012年 | 27篇 |
2011年 | 22篇 |
2010年 | 11篇 |
2009年 | 9篇 |
2008年 | 13篇 |
2007年 | 6篇 |
2006年 | 10篇 |
2005年 | 1篇 |
2004年 | 11篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 5篇 |
1999年 | 12篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1967年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有361条查询结果,搜索用时 15 毫秒
131.
Zhong X Li W Huang X Zhang L Yimamu M Raiput N Zhou Y Wang T 《Cell stress & chaperones》2012,17(4):495-505
Neonates with intrauterine growth retardation (IUGR) are susceptible to decreases in cellular immunity. In recent years, a growing body of evidence indicates that Hsp70 may serve as a danger signal to the innate immune system and promote receptor-mediated apoptosis. Using neonatal pigs with IUGR, we investigated immune function of pigs and expression of heat shock protein 70 (Hsp70), nuclear factor-kappa B (NF-κB), and forkhead box O 3a (FoxO3a) in the intestinal tract. Samples from the blood, duodenum, jejunum, and ileum of normal body weight (NBW) piglets and IUGR piglets were collected at day 7 after birth. Furthermore, to test whether Hsp70 is associated with regulation of NF-κB and FoxO3a, Hsp70 was silenced using small RNA interference (siRNA) in IEC-6 cells. Body and intestinal weights were lower in IUGR piglets than in NBW piglets (p?0.05). Proliferation of peripheral blood lymphocytes was decreased (p?0.05) in IUGR piglets. Cytokine concentrations (IFN-γ, IL-4, IL-10, IL-1, and IL-8) were lower in serum of IUGR piglets. The levels of IFN-γ and IL-10 were decreased (p?0.05) in the ileum of IUGR piglets, but IL-4 was increased (p?0.05). The expressions of Hsp70 and FoxO3a were increased, and NF-κB activity was downregulated in IUGR piglets (p?0.05). Furthermore, siRNA-mediated Hsp70 downregulation increased NF-κB activity, inhibited expression of FoxO3a, and decreased cell apoptosis. In contrast, overexpression of Hsp70 inhibited NF-κB activation. In conclusion, IUGR impairs immune functions in neonatal pigs. An inefficient immunity in IUGR piglets is associated with overexpression of Hsp70, which impairs NF-κB signaling and upregulates FoxO3a expression. 相似文献
132.
133.
134.
135.
136.
Cormels pieces of four Fusarium susceptible Gladiolus cultivara (Friendship, Peter Pears, Victor Borge and Novalux) formed friable calli when cultured in vitro on Murashige and Skoog basal medium containing various concentrations of auxin and cytokinin. The friable calli established cell suspensions. Plantlet regeneration was obtained from the control callus, control cell suspension derived callus and in vitro selected Fusarium oxysporum Schlecht. resistant cell-lines of Friendship. The in vitro cormlets showed 85-95% germination after breaking dormancy of 8weeks at 4℃. Cell suspensions of all four Gladiolus cultivara were found to be highly sensitive to fusaric acid. Gradual Increase in fusaric acid concentrations to the cell-suspension cultures decreased cell growth considerably. One albino plant was found from the second generation of the In vitro selected cell line of Friendship. The albino plant was found to be highly susceptible to F. oxysporum. The cormlets of all in vitro selected call lines of Friendship were inoculated with a conidial suspension of the F. oxysporum before planting and were also sprayed with the same spore suspension for further characterization when the height of plants was about 6cm. The four selected cell lines showed the same response whether or not they were Inoculated with conidia of the F. oxysporum. Plantlets of all of the selected call lines exhibited significant growth as compared with the control after application of conidia of the F.oxysporum. 相似文献
137.
Mazhar Ali Nasir Masood Hafiz Muhammad Rashad Javeed Ibrahim Al-Ashkar Khalid F. Almutairi Liyun Liu Muhammad Aqeel Sarwar Karthika Rajendran Ayman EL Sabagh 《Phyton》2022,91(10):2235-2248
Soil amendments containing carbonized materials increase the soil carbon reservoir, influence plant productivity,
and, ultimately, help to clean the environment. There is data on the effect of such additions on soil physicochemical
properties or plant growth, but few studies have focused on how these carbonized materials are distributed by termite species in the soil ecosystem. It is the first comprehensive study of the transportation of biochar (BC) by termite
species under tropical environmental conditions in Pakistan. The present study was carried out to test the hypothesis
that if termite species I) were involved in the distribution of biochar particles II) if yes, then how far these particles
were transported during the study period (10 days) and III) check their preference between the enriched BC (EBC)
and non-enriched BC. BC was enriched with the cattle slurry after its pyrolysis in the study. The results showed that
EBC particles were significantly more widely distributed than non-enriched BC particles, but both types of BC were
transported more than 4 cm (ring 4) within 10 days (at the end of the experiment). The current study also revealed
that EBC was easily attached to the setae, cuticle, and legs of termites, implying that it could potentially be transported over a greater distance. Furthermore, transportation of EBC over larger distances indicated a potential preference of termite species between the EBC and BC particles. During the study, however, the preference among the
termite species was also observed. Under the prevailing study conditions, the Coptotermes heimi and Heteroterme
indicola species transported the EBC further than Microtermes obesi and Odontotermes obesus. These findings
revealed that transportation preferences were observed among the four termite species. In conclusion, the current
study found that termites were involved in the distribution of BC particles, with a preference for EBC and that these
have the potential to transport BC particles more than 4 cm within 10 days. Furthermore, two species Coptotermes
heimi and Heteroterme indicola may be more suitable candidates for EBC transpiration in Pakistani soils. It was
necessary to conduct additional research into the effect of temperature on the transportation process. 相似文献
138.
139.
A simple procedure of Gossypium meristem shoot tip culture 总被引:2,自引:0,他引:2
In order to develop transgenic plants via the biolistic gun method regenerable embryogenic tissues are required. Meristem
shoot tips of 19 cultivars of cotton were cultured on several media formulations and assessed for shoot and root development.
The best shoot development was observed on media containing 0.46 mM kinetin while rooting was observed on media containing
2.68 mM NAA and 0.46 mM kinetin. No intervarietal variability was observed. A complete protocol was developed from meristem
tip culture to field transfer. This methodology is simple and replaces the existing protocols for meristem tip culture of
cotton.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献