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121.
Three types of media and a multiplex PCR procedure with a set of four primers were used to differentiate between aflatoxinogenic and non-aflatoxinogenic strains of Aspergillus flavus and Aspergillus parasiticus. Four sets of primers were the aflR, nor-1, ver-1, and omt-A genes of the aflatoxin biosynthetic pathway. Multiplex PCR showed that the four aflatoxinogenic strains gave a quadruplet pattern, indicating the presence of all the genes involved in the aflatoxin biosynthetic pathway which encode for the products. Non-aflatoxinogenic strains gave varying results with two, three, or four banding patterns. A banding pattern in seven non-aflatoxinogenic strains resulted in non-differentiation between these and aflatoxinogenic strains. 相似文献
122.
Lurie IW 《Annales de génétique》2002,45(3):127-129
Analysis of the literature showed that hypoplasia (or aplasia) of tibiae was found at least in six persons with trisomy 10q25.2-qter. Therefore, these defects should be considered as a characteristic manifestation of the distal trisomy 10q. In most of these patients, tibial abnormalities were associated with other defects of the lower extremities (hypoplastic femora, ectrodactyly, preaxial polydactyly). Upper limbs were affected in one patient (as well as in her sib without tibial defects). Most likely, segment 10q25.2-qter contains a gene which (when triplicated) leads to maldevelopment of the limbs, and tibial malformations are only one manifestation of this field defect. 相似文献
123.
124.
In this paper, the preparation, characterization and dye adsorption properties of nanocomposite (calcium alginate/organophilic montmorillonite) (CA/OMMT) were investigated. A new nanocomposite consisting of alginate and OMMT was prepared by polymerization using γ-rays irradiation as initiator. Physical characteristics of CA/OMMT were studied using X-ray diffraction (XRD), infrared spectrophotometery (IR), thermal gravimetric analysis (TGA), transmission electron microscopy (TEM) and the corresponding selected area electron diffraction (SAED). Two textile dyes, acid green B and direct pink 3B, were used as model anionic dye. Factors affecting dye sorption, such as pH, sorbent concentration and temperature of each dye solution were extensively investigated. It was found from the study that the sorption of dyes by the nanocomposite is pH-dependent and maximum sorption was obtained at pH 2. The thermodynamic data showed that dye adsorption onto alginate was spontaneous, exothermic, and a physisorption reaction. On the basis of the data of the present investigation, one could conclude that the as-prepared adsorbents exhibited excellent affinity for the dye, and can be applied to treat wastewater containing anionic dyes. 相似文献
125.
Ahmad A. Cluntun Rachit Badolia Sandra Lettlova K. Mark Parnell Thirupura S. Shankar Nikolaos A. Diakos Kristofor A. Olson Iosif Taleb Sean M. Tatum Jordan A. Berg Corey N. Cunningham Tyler Van Ry Alex J. Bott Aspasia Thodou Krokidi Sarah Fogarty Sophia Skedros Wojciech I. Swiatek Xuejing Yu Stavros G. Drakos 《Cell metabolism》2021,33(3):629-648.e10
126.
Certain genetic variations in the human population are associated with heritable diseases, and single nucleotide polymorphisms (SNPs) represent the most common form of such differences in DNA sequence. In particular, substantial interest exists in determining whether a non-synonymous SNP (nsSNP), leading to a single residue replacement in the translated protein product, is neutral or disease-related. The nature of protein structure-function relationships suggests that nsSNP effects, either benign or leading to aberrant protein function possibly associated with disease, are dependent on relative structural changes introduced upon mutation. In this study, we characterize a representative sampling of 1790 documented neutral and disease-related human nsSNPs mapped to 243 diverse human protein structures, by quantifying environmental perturbations in the associated proteins with the use of a computational mutagenesis methodology that relies on a four-body, knowledge-based, statistical contact potential. These structural change data are used as attributes to generate a vector representation for each nsSNP, in combination with additional features reflecting sequence and structure of the corresponding protein. A trained model based on the random forest supervised classification algorithm achieves 76% cross-validation accuracy. Our classifier performs at least as well as other methods that use significantly larger datasets of nsSNPs for model training, and the novelty of our attributes differentiates the model as an orthogonal approach that can be utilized in conjunction with other techniques. A dedicated server for obtaining predictions, as well as supporting datasets and documentation, is available at http://proteins.gmu.edu/automute. 相似文献
127.
A M Hilmy N A el-Domiaty A Y Daabees E M Abou Taleb 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1986,85(1):67-74
Erythrocyte count, haemoglobin content, haematocrit value, erythrocyte sedimentation rate and cadmium accumulation in some organs and tissues were estimated in toads administered 6.2 mg Cd2+/kg i.m. for 4 days. EDTA therapy caused considerable decrease in all the blood parameters studied. A striking reduction in the cadmium content of all the organs and tissues studied except the kidneys was also observed after simultaneous treatment with EDTA. 相似文献
128.
Summary High ethanol and stillage solids have been achieved using whole corn mashes. Ethanol yields of 14% (v/v) (89.5% of theory) and stillage levels of approximately 23% (w/v) were obtained in 74–90 hours using mild acid pretreatment with Aspergillus oryzae wheat bran koji saccharification. High ethanol yields were also obtained with bacterial amylase, instead of the acid treatment, when the sterilization step was omitted. The implications of ethanol fermentation process modifications are explored. 相似文献
129.