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111.
Benildo S. Cavada Mayara T. L. Silva Vinicius J. S. Osterne Vanir R. Pinto‐Junior Claudia F. Lossio Juliana C. Madeira Maria G. Pereira Rodrigo B. Leal Wandemberg P. Ferreira Kyria S. Nascimento Ana M. S. Assreuy 《Journal of molecular recognition : JMR》2020,33(11)
Lectins are a group of proteins of non‐immune origin recognized for their ability to bind reversibly to carbohydrates. Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities. Here, we report a structural and biological study of a ConA‐like mannose/glucose‐specific lectin from Canavalia bonariensis seeds, CaBo. More specifically, we evaluate the binding of CaBo with α‐methyl‐D‐mannoside (MMA) and mannose‐1,3‐α‐D‐mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation. A virtual screening was also carried out to cover a larger number of possible bindings of CaBo. In silico analysis demonstrated the stability of CaBo interaction with mannose‐type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release. These results confirm the ability of CaBo to interact with hybrid and high‐mannose N‐glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors. 相似文献
112.
Evelyn L. Jensen Christina Tschritter Peter V. C. de Groot Kristen M. Hayward Marsha Branigan Markus Dyck Rute B. G. Clemente‐Carvalho Stephen C. Lougheed 《Ecology and evolution》2020,10(8):3706-3714
Predicting the consequences of environmental changes, including human‐mediated climate change on species, requires that we quantify range‐wide patterns of genetic diversity and identify the ecological, environmental, and historical factors that have contributed to it. Here, we generate baseline data on polar bear population structure across most Canadian subpopulations (n = 358) using 13,488 genome‐wide single nucleotide polymorphisms (SNPs) identified with double‐digest restriction site‐associated DNA sequencing (ddRAD). Our ddRAD dataset showed three genetic clusters in the sampled Canadian range, congruent with previous studies based on microsatellites across the same regions; however, due to a lack of sampling in Norwegian Bay, we were unable to confirm the existence of a unique cluster in that subpopulation. These data on the genetic structure of polar bears using SNPs provide a detailed baseline against which future shifts in population structure can be assessed, and opportunities to develop new noninvasive tools for monitoring polar bears across their range. 相似文献
113.
Mikovski Andréia Izabel da Silva Nayara Tayane Silva Lázara Aline Simões Machado Mariana de Souza Barbosa Lília Cristina Reis Aryane Campos de Matos Elyabe Monteiro Viccini Lyderson Facio da Silva Souza Claudinei Machado Marcelo Dias Otoni Wagner Campos de Carvalho Ilio Fealho Rocha Diego Ismael da Silva Maurecilne Lemes 《Plant Cell, Tissue and Organ Culture》2021,145(2):239-259
Plant Cell, Tissue and Organ Culture (PCTOC) - Somaclonal variation during in vitro culture is often an undesirable phenomenon but may also be a source of genetic variation useful for breeders. The... 相似文献
114.
Ana C.A. Casarotti Daniela Teixeira Ieda M. Longo-Maugeri Mayari E. Ishimura Maria E.R. Coste Henrique T. Bianco Flavio T. Moreira Amanda F. Bacchin Maria C. Izar Iran Gonalves Jr Adriano Caixeta Gilberto Szarf Ibraim M. Pinto Francisco A. Fonseca 《Bioscience reports》2021,41(2)
Despite early reperfusion, patients with ST segment elevation myocardial infarction (STEMI) may present large myocardial necrosis and significant impairment of ventricular function. The present study aimed to evaluate the role of subtypes of B lymphocytes and related cytokines in the infarcted mass and left ventricular ejection fraction obtained by cardiac magnetic resonance imaging performed after 30 days of STEMI. This prospective study included 120 subjects with STEMI submitted to pharmacoinvasive strategy. Blood samples were collected in subjects in the first (D1) and 30th (D30) days post STEMI. The amount of CD11b+ B1 lymphocytes (cells/ml) at D1 were related to the infarcted mass (rho = 0.43; P=0.033), measured by cardiac MRI at D30. These B1 cells were associated with CD4+ T lymphocytes at D1 and D30, while B2 classic lymphocytes at day 30 were related to left ventricular ejection fraction (LVEF). Higher titers of circulating IL-4 and IL-10 were observed at D30 versus D1 (P=0.013 and P<0.001, respectively). Titers of IL-6 at D1 were associated with infarcted mass (rho = 0.41, P<0.001) and inversely related to LVEF (rho = −0.38, P<0.001). After multiple linear regression analysis, high-sensitivity troponin T and IL-6 collected at day 1 were independent predictors of infarcted mass and, at day 30, only HDL-C. Regarding LVEF, high-sensitivity troponin T and high-sensitivity C-reactive protein were independent predictors at day 1, and B2 classic lymphocytes, at day 30. In subjects with STEMI, despite early reperfusion, the amount of infarcted mass and ventricular performance were related to inflammatory responses triggered by circulating B lymphocytes. 相似文献
115.
Seminotti Bianca Roginski Ana Cristina Zanatta Ângela Amaral Alexandre Umpierrez Fernandes Thabata Spannenberger Kaleb Pinto da Silva Lucas Henrique Rodrigues Ribeiro Rafael Teixeira Leipnitz Guilhian Wajner Moacir 《Journal of bioenergetics and biomembranes》2021,53(5):525-539
Journal of Bioenergetics and Biomembranes - S-adenosylmethionine (AdoMet) predominantly accumulates in tissues and biological fluids of patients affected by liver dysmethylating diseases,... 相似文献
116.
117.
de Sousa Sylvia Morais de Oliveira Christiane Abreu Andrade Daniele Luiz de Carvalho Chainheny Gomes Ribeiro Vitória Palhares Pastina Maria Marta Marriel Ivanildo Evódio de Paula Lana Ubiraci Gomes Gomes Eliane Aparecida 《Journal of Plant Growth Regulation》2021,40(2):867-877
Journal of Plant Growth Regulation - The rising demand for agricultural commodities in developing countries has put increasing pressure on land resources for higher yields, with associated growth... 相似文献
118.
119.
Carvalho Rodrigo A. Teresa Fabrício B. Tejerina-Garro Francisco L. 《Hydrobiologia》2021,848(2):515-529
Hydrobiologia - We investigated how the riverine network influences taxonomic and functional beta diversity patterns of fish assemblages in the mainstem/headwater (lateral) and upstream/downstream... 相似文献
120.
Junior Chuctaya Andrea C. Encalada Karla S. Barragán Maria L. Torres Karla E. Rojas Valeria Ochoa-Herrera Tiago P. Carvalho 《Journal of fish biology》2021,98(4):1186-1191
Two specimens of Micromyzon akamai, an eyeless and miniaturized species previously known only from the deep channels of the eastern Amazon basin in Brazil, are reported from the Curaray River, a tributary of the Napo River in Ecuador. The new specimens are the first records of Micromyzon in the headwaters of the Amazon River and the first records of M. akamai outside Brazil. External morphological characters and a phylogenetic analysis of cytochrome c oxidase I (coI) gene support the identification of the new specimens as M. akamai. Nevertheless, the new specimens also indicate that some features previously hypothesized to be apomorphic for M. akamai are intraspecifically variable. 相似文献