全文获取类型
收费全文 | 392篇 |
免费 | 52篇 |
出版年
2022年 | 4篇 |
2020年 | 4篇 |
2014年 | 7篇 |
2013年 | 11篇 |
2012年 | 17篇 |
2011年 | 13篇 |
2010年 | 10篇 |
2009年 | 14篇 |
2008年 | 17篇 |
2007年 | 9篇 |
2006年 | 21篇 |
2005年 | 16篇 |
2004年 | 13篇 |
2003年 | 10篇 |
2002年 | 24篇 |
2001年 | 14篇 |
2000年 | 12篇 |
1999年 | 9篇 |
1998年 | 3篇 |
1996年 | 4篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1992年 | 12篇 |
1991年 | 7篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 11篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1979年 | 4篇 |
1978年 | 6篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 8篇 |
1974年 | 6篇 |
1972年 | 7篇 |
1971年 | 4篇 |
1970年 | 6篇 |
1969年 | 4篇 |
1968年 | 7篇 |
1967年 | 3篇 |
1966年 | 5篇 |
1921年 | 5篇 |
1915年 | 3篇 |
1875年 | 3篇 |
排序方式: 共有444条查询结果,搜索用时 46 毫秒
91.
92.
93.
Inman TG 《California State Journal of Medicine》1920,18(12):413-414
94.
RecA protein-facilitated DNA strand breaks. A mechanism for bypassing DNA structural barriers during strand exchange 总被引:5,自引:0,他引:5
RecA protein promotes an unexpectedly efficient DNA strand exchange between circular single-stranded DNA and duplex DNAs containing short (50-400-base pair) heterologous sequences at the 5' (initiating) end. The major mechanism by which this topological barrier is bypassed involves DNA strand breakage. Breakage is both strand and position specific, occurring almost exclusively in the displaced (+) strand of the duplex within a 15-base pair region of the heterology/homology junction. Breakage also requires recA protein, ATP hydrolysis, and homologous sequences 3' to the heterology. Although the location of the breaks and the observed requirements clearly indicate a major role for recA protein in this phenomenon, the molecular mechanism is not yet clear. The breakage may reflect a DNA structure and/or some form of structural stress within the DNA during recA protein-mediated DNA pairing which either exposes the DNA at this precise position to the action of a contaminating nuclease or induces a direct mechanical break. We also find that when heterology is located at the 3' end of the linear duplex, strand exchange is halted (without DNA breakage) about 500 base pairs from the homology/heterology junction. 相似文献
95.
96.
97.
98.
Location of DNA ends in P2, 186, P4 and lambda bacteriophage heads 总被引:10,自引:0,他引:10
When mature phage particles were suspended in a solution containing formaldehyde (0.07 m-Na+, pH 9.0, 10% HCHO for 10 min at 23 °C) and the mixture then spread for electron microscopy in the presence of 50% formamide and cytochrome c, the phage lysed and a high proportion of the DNA molecules were seen to be attached to phage tails. The phage tails were found to be attached at only one end of each DNA molecule and denaturation mapping showed that this end was unique for each of the phages P2, 186, P4 and λ. It is argued that in these mature phage particles one specific end of the DNA molecule is present at the head-tail attachment site. 相似文献
99.
100.
Cox JM Abbott SN Chitteni-Pattu S Inman RB Cox MM 《The Journal of biological chemistry》2006,281(18):12968-12975
The RecA residues Lys248 and Glu96 are closely opposed across the RecA subunit-subunit interface in some recent models of the RecA nucleoprotein filament. The K248R and E96D single mutant proteins of the Escherichia coli RecA protein each bind to DNA and form nucleoprotein filaments but do not hydrolyze ATP or dATP. A mixture of K248R and E96D single mutant proteins restores dATP hydrolysis to 25% of the wild type rate, with maximum restoration seen when the proteins are present in a 1:1 ratio. The K248R/E96D double mutant RecA protein also hydrolyzes ATP and dATP at rates up to 10-fold higher than either single mutant, although at a reduced rate compared with the wild type protein. Thus, the K248R mutation partially complements the inactive E96D mutation and vice versa. The complementation is not sufficient to allow DNA strand exchange. The K248R and E96D mutations originate from opposite sides of the subunit-subunit interface. The functional complementation suggests that Lys248 plays a significant role in ATP hydrolysis in trans across the subunit-subunit interface in the RecA nucleoprotein filament. This could be part of a mechanism for the long range coordination of hydrolytic cycles between subunits within the RecA filament. 相似文献