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11.
Leknes IL 《Diseases of aquatic organisms》2002,51(3):233-237
The ability and capacity of various tissues in platy Xiphophorus maculatus L. to take up horse-spleen ferritin injected into the blood stream are described. Ferritin was injected intraperioneally, and the cellular uptake was demonstrated as Prussian blue precipitations in tissues treated with acid ferrocyanide solutions. Ferritin was detected within the heart endocardial cells and macrophages in the trunk kidney and spleen, 1/4 h after the injection, i.e. foreign ferritin was taken up very rapidly by these cells. When the time elapsed between the ferritin injection and sacrifice exceeded 6 h, these cells, and also macrophages in the gill and intestine, were almost completely filled with ferritin. At these stages, however, the amounts of Prussian blue precipitations per volume unit of the tissue were much larger in the heart than in the other organs studied in the present work, i.e. the endocardial tissue seems to play an important role in the clearance of the blood circulation in this species. We suggest that this tissue in platy is specialized to endocytose waste and foreign macromolecules, including pathogenic particles, from the blood stream. The eosinophilic and neutrophilic granulocytes do not appear to take up foreign ferritin, i.e. these cells may play no endocytotic role in the clearance of foreign macromolecules in platy. 相似文献
12.
The clinicopathological and gene expression patterns associated with ulceration of primary melanoma
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Rosalyn Jewell Faye Elliott Jonathan Laye Jérémie Nsengimana John Davies Christy Walker Caroline Conway Angana Mitra Mark Harland Martin G. Cook Andy Boon Sarah Storr Sabreena Safuan Stewart G. Martin Karin Jirström Håkan Olsson Christian Ingvar Martin Lauss Tim Bishop Göran Jönsson Julia Newton‐Bishop 《Pigment cell & melanoma research》2015,28(1):94-104
Ulceration of primary melanomas is associated with poor prognosis yet is reported to predict benefit from adjuvant interferon. To better understand the biological processes involved, clinicopathological factors associated with ulceration were determined in 1804 patients. From this cohort, 348 primary tumor blocks were sampled to generate gene expression data using a 502‐gene cancer panel and 195 blocks were used for immunohistochemistry to detect macrophage infiltration and vessel density. Gene expression results were validated using a whole genome array in two independent sample sets. Ulceration of primary melanomas was associated with more proliferative tumors, tumor vessel invasion, and increased microvessel density. Infiltration of tumors with greater number of macrophages and gene expression pathways associated with wound healing and up‐regulation of pro‐inflammatory cytokines suggests that ulceration is associated with tumor‐related inflammation. The relative benefit from interferon reported in patients with ulcerated tumors may reflect modification of signaling pathways involved in inflammation. 相似文献
13.
Gunilla Journath Niklas Hammar Stig Elofsson Anette Linnersj? Max Vikstr?m G?ran Walldius Ingvar Krakau Peter Lindgren Ulf de Faire Mai-Lis Hellénius 《PloS one》2015,10(11)
Background
In 1988, a cardiovascular prevention program which combined an individual and a population-based strategy was launched within primary health-care in Sollentuna, a municipality in Stockholm County. The aim of this study was to investigate time trends in the incidence of and mortality from acute myocardial infarction and all-cause mortality in Sollentuna compared with the rest of Stockholm County during a period of two decades following the implementation of a cardiovascular prevention program.Materials and Methods
The average population in Sollentuna was 56,589 (49% men) and in Stockholm County (Sollentuna included) 1,795,504 (49% men) during the study period of 1987–2010. Cases of hospitalized acute myocardial infarction and death were obtained for the population of Sollentuna and the rest of Stockholm County using national registries of hospital discharges and deaths. Acute myocardial infarction incidence and mortality were estimated using the average population of Sollentuna and Stockholm in 1987–2010.Results
During the observation period, the incidence of acute myocardial infarction decreased more in Sollentuna compared with the rest of Stockholm County in women (-22% vs. -7%; for difference in slope <0.05). There was a trend towards a greater decline in Sollentuna compared to the rest of Stockholm County in the incidence of acute myocardial infarction (in men), acute myocardial mortality, and all-cause mortality but the differences were not significant.Conclusion
During a period of steep decline in acute myocardial infarction incidence and mortality in Stockholm County the municipality of Sollentuna showed a stronger trend in women possibly compatible with favorable influence of a cardiovascular prevention program.Trial Registration
ClinicalTrials.gov NCT02212145 相似文献14.
Charlotte Welinder Krzysztof Paw?owski Yutaka Sugihara Maria Yakovleva G?ran J?nsson Christian Ingvar Lotta Lundgren Bo Baldetorp H?kan Olsson Melinda Rezeli Bo Jansson Thomas Laurell Thomas Fehniger Balazs D?me Johan Malm Elisabet Wieslander Toshihide Nishimura Gy?rgy Marko-Varga 《PloS one》2015,10(4)
Malignant melanoma has the highest increase of incidence of malignancies in the western world. In early stages, front line therapy is surgical excision of the primary tumor. Metastatic disease has very limited possibilities for cure. Recently, several protein kinase inhibitors and immune modifiers have shown promising clinical results but drug resistance in metastasized melanoma remains a major problem. The need for routine clinical biomarkers to follow disease progression and treatment efficacy is high. The aim of the present study was to build a protein sequence database in metastatic melanoma, searching for novel, relevant biomarkers. Ten lymph node metastases (South-Swedish Malignant Melanoma Biobank) were subjected to global protein expression analysis using two proteomics approaches (with/without orthogonal fractionation). Fractionation produced higher numbers of protein identifications (4284). Combining both methods, 5326 unique proteins were identified (2641 proteins overlapping). Deep mining proteomics may contribute to the discovery of novel biomarkers for metastatic melanoma, for example dividing the samples into two metastatic melanoma “genomic subtypes”, (“pigmentation” and “high immune”) revealed several proteins showing differential levels of expression. In conclusion, the present study provides an initial version of a metastatic melanoma protein sequence database producing a total of more than 5000 unique protein identifications. The raw data have been deposited to the ProteomeXchange with identifiers PXD001724 and PXD001725. 相似文献
15.
During storage of moist animal feed, growth of detrimental fungi causing spoilage, or being mycotoxigenic or pathogenic, is
a severe problem. Addition of biopreservative yeasts or lactic acid bacteria can significantly reduce this problem. However,
their use requires several careful considerations. One is the safety to the animal, humans and the environment, tightly connected
to legal aspects and the need for pre-market authorisation when supplementing feed with microorganisms. Although both yeasts
and lactic acid bacteria are considered comparatively safe organisms due to low production of toxic metabolites, it is of
great importance to understand the mechanisms behind the biopreservative abilities. Another important issue concerns practical
aspects, such as the economic production of large amounts of the organisms and the development of a suitable formulation giving
the organisms a long shelf life. These aspects are discussed and a recommendation of this review is that both safety and formulation
aspects of a specific microbe should be considered at an early stage in the selection of new organisms with biopreservation
potential. 相似文献
16.
17.
Eosinophilic granule cells and endocytic cells in intestinal wall of pearl gouramy (Anabantidae: Teleostei) 总被引:1,自引:0,他引:1
Leknes IL 《Fish & shellfish immunology》2007,23(4):897-900
The localization and frequency of eosinophilic granule cells (EGCs) and endocytic cells in the intestinal wall of a teleost, pearl gouramy (Trichogaster leeri), a stomach-containing anabantid, are described. EGCs occurred in high numbers in lamina propria and epithelial layer throughout the entire intestine. They often embraced blood capillaries, but were unable to take up foreign ferritin, i.e. they seem not to play any blood cleansing role in this tissue. Only some of the EGCs granules reacted with Schmorl's solution, suggesting that they are at a different stage of development or of different types in these cells. The granule content is probably secreted close to the intestine blood stream where it may play important local immunological roles. The present results suggest that the number of intestinal EGCs can be high in teleosts even in species with a putative well-developed acid-based defence in the digestive tract. Also, endocytic cells capable of taking up and storing much foreign ferritin were frequently seen in this tissue; such cells may play significant cleansing and scavenger roles throughout the entire intestine in pearl gouramy. 相似文献
18.
19.
Understanding the fragmentation process in MS/MS experiments is vital when trying to validate the results of such experiments, and one way of improving our understanding is to analyze existing data. We here present our findings from an analysis of a large and diverse data set of MS/MS-based peptide identifications, in which each peptide has been identified from multiple spectra, recorded on two commonly used types of electrospray instruments. By analyzing these data we were able to study fragmentation variability on three levels: (i) variation in detection rates and intensities for fragment ions from the same peptide sequence measured multiple times on a single instrument; (ii) consistency of rank-based fragmentation patterns; and (iii) a set of general observations on fragment ion occurrence in MS/MS experiments, regardless of sequence. Our results confirm that substantial variation can be found at all levels, even when high-quality identifications are used and the experimental conditions as well as the peptide sequences are kept constant. Finally, we discuss the observed variability in light of ongoing efforts to create spectral libraries and predictive software for target selection in targeted proteomics. 相似文献
20.