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431.
Eckart Görschen Marina Dunaeva Bettina Hause Ingeborg Reeh Claus Wasternack Benno Parthier 《Planta》1997,202(4):470-478
In this paper we report the in-planta activity of the ribosome-inactivating protein JIP60, a 60-kDa jasmonate-induced protein
from barley (Hordeum vulgare L.), in transgenic tobacco (Nicotiana tabacum L.) plants. All plants expressing the complete JIP60 cDNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter
exhibited conspicuous and similar phenotypic alterations, such as slower growth, shorter internodes, lanceolate leaves, reduced
root development, and premature senescence of leaves. Microscopic inspection of developing leaves showed a loss of residual
meristems and higher degree of vacuolation of mesophyll cells as compared to the wild type. When probed with an antiserum
which was immunoreactive against both the N- and the C-terminal half of JIP60, a polypeptide with a molecular mass of about
30 kDa, most probably a processed JIP60 product, could be detected. Phenotypic alterations could be correlated with the differences
in the detectable amount of the JIP60 mRNA and processed JIP60 protein. The protein biosynthesis of the transformants was
characterized by an increased polysome/monosome ratio but a decreased in-vivo translation activity. These findings suggest
that JIP60 perturbs the translation machinery in planta. An immunohistological analysis using the JIP60 antiserum indicated
that the immunoreactive polypeptide(s) are located mainly in the nucleus of transgenic tobacco leaf cells and to a minor extent
in the cytoplasm.
Received: 31 July 1996 / Accepted: 18 February 1997 相似文献
432.
Jan Bauer Sigrid R. Ruuls Ingeborg Huitinga Christine D. Dijkstra 《The Histochemical journal》1996,28(2):83-97
Summary In this review the role of various subpopulations of macrophages in the pathogenesis of experimental autoimmune encephalomyetitis
is discussed. Immunohistochemistry with macrophage markers shows that in this disease different populations of macrophages
(i.e. perivascular cells, microglia and infiltrating blood-borne macrophages) are present in the central nervous system. These
subpopulations partially overlap in some functional activity while other activities seem to be restricted to a distinct subpopulation,
indicating that these subpopulations have different roles in the pathogenesis of encephalomyelitis. The studies discussed
in this review reveal that immunocytochemical and morphological studies, combined with new techniques such asin situ nick translation and experimental approaches like the use of bone marrow chimeras and macrophage depletion techniques, give
valuable information about the types and functions of cells involved in central nervous system inflammation. The review is
divided in three parts. In the first part the experimental autoimmune encephalomyelitis model is introduced. The second part
gives an overview of the origin, morphology and functions of the various subpopulations. In the third part the role of these
subpopulations is discussed in relation to the various stages (i.e. preclinical, clinical and recovery) of the experimental
disease. 相似文献
433.
434.
Ingeborg Haug 《Mycological Progress》2002,1(2):167-178
Identification of the fungi forming ectomycorrhiza is still a great challenge. Ectomycorrhizae of Picea abies, collected in southwest Germany during several years and described as morphotypes, were identified using LSU and ITS sequences.
To this the fungal sequences amplified from the mycorrhizae were compared with sequences from identified sporocarps. The fungal
partner of Piceirhiza gelatinosa was identified as Hygrophorus olivaceoalbus, the fungal partner of Piceirhiza rosa-nigrescens was identified as Dermocybe cf. semisanguinea, and the fungal partner of a white mycorrhiza, described here for the first time, belongs to the Hebeloma velutipes group (Hebeloma crustuliniforme complex). Identification to genus level was possible for Piceirhiza lanuginosa where a Cortinarius-species is the fungal partner. A Tomentella-species forms a newly described light-brown mycorrhiza. Identification to family and to order-level was achieved for a milky-dull
silvery mycorrhiza (Cortinariaceae), and Piceirhiza globulifera (Aphyllophorales), respectively. Ten samples of white, slightly bent mycorrhizae were formed by 8 different Cortinarius-species, including among others Cortinarius traganus, C. delibutus, and C. brunneus. The fungal partners of several brown, smooth mycorrhizae displaying only a Hartig net are Ascomycetes, among these are Wilcoxina cf. mikolae and Hymenoscyphus cf. ericae. 相似文献