全文获取类型
收费全文 | 1431篇 |
免费 | 126篇 |
专业分类
1557篇 |
出版年
2022年 | 9篇 |
2021年 | 26篇 |
2020年 | 19篇 |
2019年 | 15篇 |
2018年 | 26篇 |
2017年 | 27篇 |
2016年 | 31篇 |
2015年 | 74篇 |
2014年 | 81篇 |
2013年 | 101篇 |
2012年 | 104篇 |
2011年 | 92篇 |
2010年 | 67篇 |
2009年 | 42篇 |
2008年 | 68篇 |
2007年 | 66篇 |
2006年 | 58篇 |
2005年 | 37篇 |
2004年 | 43篇 |
2003年 | 42篇 |
2002年 | 34篇 |
2001年 | 32篇 |
2000年 | 24篇 |
1999年 | 21篇 |
1998年 | 11篇 |
1997年 | 10篇 |
1996年 | 14篇 |
1995年 | 12篇 |
1994年 | 9篇 |
1993年 | 8篇 |
1992年 | 25篇 |
1991年 | 21篇 |
1990年 | 19篇 |
1989年 | 27篇 |
1988年 | 17篇 |
1987年 | 15篇 |
1986年 | 15篇 |
1985年 | 8篇 |
1984年 | 12篇 |
1982年 | 11篇 |
1981年 | 11篇 |
1980年 | 8篇 |
1979年 | 8篇 |
1975年 | 9篇 |
1973年 | 15篇 |
1972年 | 11篇 |
1971年 | 9篇 |
1970年 | 9篇 |
1968年 | 7篇 |
1967年 | 7篇 |
排序方式: 共有1557条查询结果,搜索用时 8 毫秒
971.
972.
A genome‐scale screen reveals context‐dependent ovarian cancer sensitivity to miRNA overexpression 下载免费PDF全文
Benjamin B Shields Chad V Pecot Hua Gao Elizabeth McMillan Malia Potts Christa Nagel Scott Purinton Ying Wang Cristina Ivan Hyun Seok Kim Robert J Borkowski Shaheen Khan Cristian Rodriguez‐Aguayo Gabriel Lopez‐Berestein Jayanthi Lea Adi Gazdar Keith A Baggerly Anil K Sood Michael A White 《Molecular systems biology》2015,11(12)
Large‐scale molecular annotation of epithelial ovarian cancer (EOC) indicates remarkable heterogeneity in the etiology of that disease. This diversity presents a significant obstacle against intervention target discovery. However, inactivation of miRNA biogenesis is commonly associated with advanced disease. Thus, restoration of miRNA activity may represent a common vulnerability among diverse EOC oncogenotypes. To test this, we employed genome‐scale, gain‐of‐function, miRNA mimic toxicity screens in a large, diverse spectrum of EOC cell lines. We found that all cell lines responded to at least some miRNA mimics, but that the nature of the miRNA mimics provoking a response was highly selective within the panel. These selective toxicity profiles were leveraged to define modes of action and molecular response indicators for miRNA mimics with tumor‐suppressive characteristics in vivo. A mechanistic principle emerging from this analysis was sensitivity of EOC to miRNA‐mediated release of cell fate specification programs, loss of which may be a prerequisite for development of this disease. 相似文献
973.
Sebastien Hannedouche Valerie Beck Juliet Leighton-Davies Martin Beibel Guglielmo Roma Edward J. Oakeley Vincent Lannoy Jerome Bernard Jacques Hamon Samuel Barbieri Inga Preuss Marie-Christine Lasbennes Andreas W. Sailer Thomas Suply Klaus Seuwen Christian N. Parker Frederic Bassilana 《The Journal of biological chemistry》2013,288(38):27434-27443
974.
T. Nagel S. Loerakker C.W.J. Oomens 《Computer methods in biomechanics and biomedical engineering》2013,16(5):585-597
Pressure induced deep tissue injury (DTI) is a severe form of pressure ulcers that is hard to detect in early stages and difficult to prevent and treat. High prevalence figures are partly due to a lack of understanding of pathological pathways involved in DTI. The aim of this study was to investigate, whether changes in material properties of damaged tissue can play a role in DTI aetiology. A numerical model was developed based on muscle microstructure and tissue engineering experiments. A time dependent damage law was proposed and stiffening of dead cells incorporated. The results obtained in the microstructural investigations were used to include the stiffening information in a pre-existing macroscopic model based on animal experiments, which correlated strains to tissue damage measured in the tibialis anterior muscle in rat limbs. With the modelling approach employed in this paper, the damaged area in the rat limb models increased up to 1.65-fold and the rate of damage progression was up to 2.1 times higher in microstructural simulations when stiffening was included. 相似文献
975.
Zamyatina A. V. Rudenko N. V. Karatovskaya A. P. Shepelyakovskaya A. O. Siunov A. V. Andreeva-Kovalevskaya Zh. I. Nagel A. S. Salyamov V. I. Kolesnikov A. S. Brovko F. A. Solonin A. S. 《Russian Journal of Bioorganic Chemistry》2020,46(6):1214-1220
Russian Journal of Bioorganic Chemistry - Among the panel of monoclonal antibodies to the recombinant protein HlyIICTD Bacillus cereus an antibody was found capable of forming an immune complex... 相似文献
976.
977.
978.
979.
980.
O.G. Nagel M.P. Molina J.C. Basílico M.L.Zapata R.L. Althaus 《Letters in applied microbiology》2009,48(6):744-749
Aims: To use experimental design techniques and a multiple logistic regression model to optimize a microbiological inhibition test with dichotomous response for the detection of Penicillin G in milk.
Methods and Results: A 23 × 22 robust experimental design with two replications was used. The effects of three control factors (V: culture medium volume, S: spore concentration of Geobacillus stearothermophilus , I: indicator concentration), two noise factors (Dt: diffusion time, Ip: incubation period) and their interactions were studied. The V, S, Dt, Ip factors and V × S, V × Ip, S × Ip interactions showed significant effects.
Conclusions: The use of 100 μ l culture medium volume, 2 × 105 spores ml−1 , 60 min diffusion time and 3 h incubation period is recommended. In these elaboration conditions, the penicillin detection limit was of 3·9 μ g l−1 , similar to the maximum residue limit (MRL). Of the two noise factors studied, the incubation period can be controlled by means of the culture medium volume and spore concentration.
Significance and Impact of the Study: We were able to optimize bioassays of dichotomous response using an experimental design and logistic regression model for the detection of residues at the level of MRL, aiding in the avoidance of health problems in the consumer. 相似文献
Methods and Results: A 2
Conclusions: The use of 100 μ l culture medium volume, 2 × 10
Significance and Impact of the Study: We were able to optimize bioassays of dichotomous response using an experimental design and logistic regression model for the detection of residues at the level of MRL, aiding in the avoidance of health problems in the consumer. 相似文献