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921.
The chlorophyll-protein complexes of the yellow alga Synura petersenii (Chrysophyceae) and the yellow-green alga Tribonema aequale (Xanthophyceae) were studied. The sodiumdodecylsulfate/sodiumdesoxycholate solubilized photosynthetic membranes of these species yielded three distinct pigment-protein complexes and a non-proteinuous zone of free pigments, when subjected to SDS polyacrylamid gel electrophoresis. The slowest migrating protein was identical to complex I (CP I), the P-700 chlorophyll a-protein, which possessed 60 chlorophyll a molecules per reaction center in Tribonema and 108 in Synura. The zone of intermediate mobility contained chlorophyll a and carotenoids. The absorption spectrum of this complex was very similar to the chlorophyll a-protein of photosystem II (CP a), which is known from green plants. The fastest migrating pigment protein zone was identified as a light-harvesting chlorophyll-protein complex. In Synura this protein was characterized by the content of chlorophyll c and of fucoxanthin. Therefore this complex will be named as LH Chl a/c-fucocanthin protein. In addition to the separation of the chlorophyll-protein complexes the cellular contents of P-700, cytochrome f (bound cytochrome) and cytochrome c-553 (soluble cytochrome) were measured. The stoichiometry of cytochrome f: cytochrome c-553:P-700 was found to be 1:4:2.4 in Tribonema and 1:6:3.4 in Synurá.Abbreviations CP a
chlorophyll a-protein of photosystem II
- CP I
P-700 chlorophyll a-protein
- FP
free pigment
- LH Chl a/c
light-harvesting chlorophyll a/c-protein
- PAGE
polyacrylamidgelelectrophoresis
- SDS
Sodiumdodecylsulfate
- SDOC
sodium-desoxycholate 相似文献
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Ina Schulte E-juan Zhang Zhong-ji Meng Rong-juan Pei Mengji Lu Michael Roggendorf 《中国病毒学》2008,23(2):107-115
The woodchuck model is an excellent animal model to study hepadnaviral infection. The new progresses in this model made possible to examine the T-cell mediated immune responses in acute and chronic hepadnaviral infection. Recently, a new assay for cytotoxic T-cells based on detection of CD107 was established for the woodchuck model. In addition, new immunotherapeutic approaches based on combination of potent antiviral treatment and DNA-protein vaccines were proven to be useful for treatment of chronic hepatitis B. 相似文献
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Groups of Hodotermes mossambicus reproductives exhibit social behaviour. Maintenance of this behaviour by an individual depends on frequent contact of its body by the antennae of other termites. The antennae of the recipient do not play a rôle. Pairs on the other hand exhibit antisocial behaviour. Development of pair behaviour occurs when antenna to body contact is reduced or absent. Furthermore, at least the proximal parts of the antennae are required for full development of pair behaviour. Pheromones are not implicated in the change from group to pair behaviour. 相似文献
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Feng-Liu Wang Ya-Lan Tan Lukas Wallrad Xin-Qiao Du Anna Eickelkamp Zhi-Fang Wang Ge-Feng He Felix Rehms Zhen Li Jian-Pu Han Ina Schmitz-Thom Wei-Hua Wu Jörg Kudla Yi Wang 《Developmental cell》2021,56(6):781-794.e6
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