太白山锐齿栎树干基部两种蚂蚁的筑巢生态学及其蚁巢、体表真菌组成研究

本文研究了太白山锐齿栎景观林带树干基部筑巢的两种蚂蚁的筑巢生态学及其巢内真菌的组成.研究结果表明:亮腹黑褐蚁Formica gagatoides Ruzsky是锐齿栎林中的优势蚁种,可在地表及树干上活动,主要取食栎树枝条上蚜虫所分泌的蜜露及其他昆虫和植物材料;盘腹蚁Aphaenogaster sp.仅在蚁巢周围活动,捕...     

连栽土壤对杉木幼苗生长影响的研究

连栽土壤对杉木幼苗生长影响的研究马越强廖利平杨跃军汪思龙高洪陈楚莹（中国科学院沈阳应用生态研究所,１１００１５）刘更有（湖南省会同县林科所,４１８３０７）ＥｆｅｃｔｓｏｆＲｅｐｌａｎｔＳｏｉｌｏｎｔｈｅＧｒｏｗｔｈｏｆＣ．ｌａｎｃｅｏｌａｔａＳｅｄｌ...     

利用激光扫描共聚焦显微镜研究视皮层脑片长时程增强过程中核酸的变化

本文使用 1 5— 2 0天龄幼年大鼠视皮层脑片的标本 ,在通氧状态下 ,用荧光探针 AO染色 ,激光扫描共聚焦显微镜对全脑片不同层面进行共聚焦断层扫描 ,沿纵轴每 2 0μm扫一次 ,共扫描 1 6次。再利用总值方式的投影算法对其三维重建 ,分析幼年大鼠视皮层脑片长时程增强过程中核酸的变化。     

Ginsenoside Re reduces insulin resistance through inhibition of c-Jun NH2-terminal kinase and nuclear factor-kappaB

Ginsenoside Re (Re), a compound derived from Panax ginseng, shows an antidiabetic effect. However, the molecular basis of its action remains unknown. We investigated insulin signaling and the antiinflammatory effect by Re in 3T3-L1 adipocytes and in high-fat diet (HFD) rats to dissect its anti-hyperglycemic mechanism. Glucose uptake was measured in 3T3-L1 cells and glucose infusion rate determined by clamp in HFD rats. The insulin signaling cascade, including insulin receptor (IR) beta-subunit, IR substrate-1, phosphatidylinositol 3-kinase, Akt and Akt substrate of 160 kDa, and glucose transporter-4 translocation are examined. Furthermore, c-Jun NH(2)-terminal kinase (JNK), MAPK, and nuclear factor (NF)-kappaB signaling cascades were also assessed. The results show Re increases glucose uptake in 3T3-L1 cells and glucose infusion rate in HFD rats. The activation of insulin signaling by Re is initiated at IR substrate-1 and further passes on through phosphatidylinositol 3-kinase and downstream signaling cascades. Moreover, Re demonstrates an impressive suppression of JNK and NF-kappaB activation and inhibitor of NF-kappaBalpha degradation. In conclusion, Re reduces insulin resistance in 3T3-L1 adipocytes and HFD rats through inhibition of JNK and NF-kappaB activation.     

The DLC-1 -29A/T polymorphism is not associated with nasopharyngeal carcinoma risk in Chinese population

Deleted in liver cancer-1 (DLC-1), encoding a Rho GTPase-activating protein (GAP), is considered as a promising candidate tumor suppressor gene in nasopharyngeal carcinoma (NPC). The single-nucleotide polymorphism (SNP) -29A/T upstream of ATG start codon was found when gene mutation profile of DLC-1 in NPC was analyzed. To evaluate the correlation between SNP -29A/T in the promoter region of DLC-1 gene and risk of NPC, a total of 521 samples from a Chinese population, including 320 healthy individuals and 201 NPC patients, were collected for SNP analysis by PCR-single-strand conformation polymorphism and sequencing. The differences in allele and genotype frequencies between NPC patients and controls were tested using logistic regression statistical method. No significant differences were found in allele or genotype frequencies between NPC patients and controls or among different NPC clinical stages. Hence, our data indicate that the SNP -29A/T of DLC-1 gene is not associated with NPC susceptibility.     

稻瘿蚊种群DNA指纹及水稻抗稻瘿蚊分子标记育种技术研究

文章综述经10年(1996~2005)研究建立的亚洲稻瘿蚊Orseolia oryzae Wood-Mason种群DNA指纹检测技术和水稻抗稻瘿蚊分子育种技术的研究成果。其核心技术包括5点:(1)在国际和国内首先用AFLP方法研究亚洲5国(中国,印度,斯里兰卡,尼泊尔和老挝)及广东省7个地点亚洲稻瘿蚊种群DNA指纹及其生物多样性;(2)分别用RAPD和微卫星SSR技术对来源于我国的水稻资源大秋其的抗亚洲稻瘿蚊基因Gm6进行精细定位;(3)分别用与Gm6基因紧密连锁的STS分子标记和SSR标记,建立了分子标记辅助选择(MAS)方法选育抗稻瘿蚊新品种的技术体系;(4)应用建立的分子标记辅助选育新技术选育出一批抗亚洲稻瘿蚊中国种群的新品系,创造出一批新种质,包括抗蚊18号、抗蚊软占等达国优3级的抗稻瘿蚊新品系6个;(5)将Gm6基因导入杂交稻恢复系,选育出4个抗稻瘿蚊两系杂交稻新组合培矮64S/KG18,培矮64S/KI41,培矮64S/AK7,培矮64S/03W16(国优2级)和1个三系杂交稻新组合抗蚊博优。其中江西省宁都市名林水稻研究所应用本研究鉴定出的带有Gm6基因的品系抗蚊青占作为抗性亲本,培育出的2个抗稻瘿蚊的两系杂交稻组合安两优青占和培两优抗占通过省级品种审定,率先选育出抗稻瘿蚊种群的杂交稻。该项研究成果为控制华南稻瘿蚊的灾害发生提供新的技术措施,选育的抗稻瘿蚊品种和新种质,在华南广为应用,取得显著的经济效益。     

An expression profile of human α-lactalbumin in the milk of transgenic mouse

a-lactalbumin(a-Lac),amajorwheyprotein,isacalciummetalloprotein,thathasbeenfoundinallmilksstudiedsofar.ItinteractswithUDP-galactosyl-transferasetoformthelactosesynthetaseandthusmightbeakeyproteinforlactogenesis.Lactosesyn-thetaseispostulatedtobetherate-limitingenzymeforlactosebiosynthesis.Theincreaseda-Lacactivitycanproducesufficientlactosesynthetaseforthesynthesisoflactose,andinmilkyieldbydrawingwaterintomilk,sincelactoseisanosmoreactivemolecule.Transgenicswineoverexpressingbovinea-lactalbu…     

玉米幼苗萎蔫过程中某些理化性质变化的研究

玉米幼苗在暂时性萎蔫期间失水速率相对较慢,可溶性糖,脯氨酸等渗透调度物质的积累较多,ＳＯＤ活性增加,而在永久性萎缩期间失水速率较快,渗透调节物质减少,ＳＯＤ活性下降,在玉米幼苗萎蔫的全过程中,叶绿素,蛋白质含量及ＣＡＴ,ＰＯＤ活性等迅速下降,质膜透性增大,膜脂过氧化加剧。     

黄檀属模式指定及新异名

指定了黄檀属Dalbergia黄檀D．hupeana Hance、滇黔黄檀D．yunnanensis Franch．、D．collettii Prain和多裂黄檀D．rimosa Roxb．四个名称的后选模式。分别将上海黄檀D．sacerdotum Prain、高原黄檀D.yunnanensis var.collettii（Prain）Thoth．和勐梭黄檀D．mengsuoensis Y.Y.Qian处理为黄檀D.hupeana、滇黔黄檀D．yunnanensis和多裂黄檀D．rimosa的异名。     

腺苷产生菌Xn151菌株的选育及发酵条件研究

以肌苷产生菌枯草杆菌GMI-741(Ade~(--))为出发菌株,通过多因子诱变选育出具有黄嘌呤、鸟嘌呤双重营养缺陷型、丧失腺嘌呤脱氨酶的腺苷产生菌Xn151。以Xn151为发酵菌株,采用正交实验设计法对其发酵基础培养基进行优化,确定最佳发酵配方。利用此培养及配方摇瓶发酵72h,腺苷产量可达12.3g/L。