Ergebnisse von Bestimmungen menschlicher Serum-Transferrin-Typen

Zusammenfassung Es wird über die Ergebnisse der in Ungarn erstmals durchgeführten Serum-Transferrin-Typen-Bestimmungen an 1007 Personen berichtet. Die Untersuchungen wurden mit der horizontalen Stärkegelelektrophorese durchgeführt. In 5 Fällen wurden Varianten gefunden, die sich als Typ B_0-1C bzw. B₀C erwiesen. Weiterhin wird über die Ergebnisse von Tf-Untersuchungen in Fällen strittiger Vaterschaft berichtet. In einer 2-Männer-Sache gelang es, eine Zuordnung höchstwahrscheinlich zu machen.

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Distribution of serum transferrin groups in Hungrary and their application in paternity proceedings

Summary Results of human serum transferrin-type determinations carried out on 1007 persons the first time in Hungary are reported. The determinations were carried out by way of the horizontal starchgel electrophorese according to Smithies modified by Ashton. In 5 cases variants were found migrating faster than the common type Tf C proving at the identification as type B_0-1 respectively B₀. That is, the frequency of the Tf-variants in Hungary are estimated about 0.5%. The significance of Tf-type determinations in clearing up disputed paternity is discussed. Also the results of determinations of this kind in a juridicial case are reported. It was about a 2-men case. Paternity of the witness was disclosed by way of Tf-type determination, that of the defendant, however, proved.

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Vorstand: Prof. Dr. med. E. Somogyi

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Vorstand: Prof. Dr. J. Márkus     

A propos de quelques groupements végétaux observés entre monaco et gênes

Résumé Le climat chaud et humide de la Ligurie méditerranéenne détermine la réapparition de quelques groupements végétaux connus de la Catalogne et rares ou nuls en Languedoc: Inulo-Oryzopsietum miliaceae, Cichorio-Sporoboletum poiretii, Hyparrhenietum hirto-pubescentis, Rubo-Coriarietum.Le caractère particulier de la végétation ligure a été exprimé par des groupements spéciaux: Pistacio-Rhamnetum alaterni, qui remplace le Quercetum cocciferae, groupements à Ostrya carpinifolia et à Brachypodium pinnatum des endroits frais, groupements buissonnants à Erica arborea et Genista germanica, à Rosmarinus et Genista cinerea, Asplenio-Campanuletum macrorhizae des rochers, etc.

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Summary The warm and humid climate of the Mediterranean Liguria determines the reappearance of some communities, known in Catalonia, and seldom or not existing in Languedoc: Inulo-Oryzopsietum miliaceae, Cichorio-Sporoboletum poiretii, Hyparrhenietum hirto-pubescentis, Rubo-Coriarietum.The particular character of the Liguric vegetation is expressed in special communities: Pistacio-Rhamnetum alaterni, which replaces the Quercetum cocciferae, communities with Ostrya carpinifolia and with Brachypodium pinnatum in the fresh places, bushy communities with Erica arborea and Genista germanica, with Rosmarinus and Genista cinerea, Asplenio-Campanuletum macrorhizae of the rocks, and so on.

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Zusammenfassung Das warme und feuchte Klima der mediterranen Ligurie bestimmt das Wiedererscheinen einiger Pflanzengesellschaften, die in Katalonien bekannt sind, aber selten oder nicht in Languedoc vorkommen: Inulo-Oryzopsietum miliaceae, Cichorio-Sporoboletum poiretii, Hyparrhenietum hirto-pubescentis, Rubo-Coriarietum.Der eigenartige Charakter der ligurischen Vegetation ist in besonderen Pflanzengesellschaften ausgedrückt: Pistacio-Rhamnetum alaterni, das das Quercetum cocciferae ersetzt, Gesellschaften mit Ostrya carpinifolia und mit Brachypodium pinnatum der frischen Standorte, Erica arborea-Genista germanica-Silikatbodengebüsche, Rosmarinus-Genista cinerea-Kalkbodengebüsche, Asplenio-Campanuletum macrorhizae der Felsen usw.

     

Seasonal changes in growth and standard metabolic rate of juvenile perch, Perca fluviatilis L.

The maximum growth rate of juvenile perch, PercaJuviatilis L., at different constant temperatures and in naturally changing day-lengths was studied in the laboratory. Standard metabolic rate was studied in starvation experiments at constant temperatures under short- and long-day conditions. Growth occurred in temperatures above 8 to 10°C. In winter, from mid-October until mid-April, maximal growth was considerably reduced and was relatively slow but constant. The standard metabolic rate was reduced c . 50% under short-day conditions. The seasonal change in metabolic rates, presumably controlled by an endogenous rhythm, was considered to be an adaptation to low food availability during the short winter days.     

Synergistic effects of ethanol and temperature on yeast mitochondria

At temperatures lower than 37°C, the ethanol inhibition constant (Ki) for growth or fermentation inrho ⁺ cells of theSaccharomyces cerevisiae strain S288C was always higher (1.1M) than inrho ^– mutants (0.7M). At 37°C these differences disappeared, and both strains were equally inhibited by ethanol (Ki=0.7m). Mitochondrial activity can be inhibited by high ethanol concentration and temperature. In fact, the stronger inhibition by ethanol of therho ⁺ strain at 37°C was due to the fact that, under these conditions, this strain loses the advantage conferred by mitochondrial activity since the induction ofrho ^– cells in the population is very high. This does not result in an increase in the frequency ofrho ^– mutants because of the poor viability of these mutants in conditions of high temperature and ethanol. In consequence, S288C strain becomes as strongly inhibited by ethanol as therho ^– mutant strains. Differences in viability were not related to the fatty acids and ergosterol composition of the strain. In the presence of ethanol, bothrho ⁺ andrho ^– strains modified their lipids in the same way, but these changes did not improve their ethanol tolerance. They were not due to differences in adaptation to ethanol either, since after successive transfers in ethanol, growth () and fermentation () rates in therho ^– mutants were increasingly inhibited with time, whereas in the S288C strain inhibition of and by ethanol remained unaltered. Rather,rho ^– mutants are less viable thanrho ⁺ cells because of the inability of the former to respire. At 37°C the Ki increased to 0.9M ethanol either when mitochondrial from highly ethanol-tolerant wine yeasts were transferred torho ^– mutants of the strain S288C or when the mitochondria of strain S288C were preadapted by growing the strain in glycerol instead of glucose before it was cultivated in ethanol.     

Establishment of transformed root cultures of Perezia cuernavacana producing the sesquiterpene quinone perezone

Summary The sesquiterpene quinone currently known as perezone is abundantly produced by the roots of Perezia cuernavacana. This compound is of biotechnological interest since it may be used as a pigment and has several pharmacological properties. In this work we demonstrate that perezone is also produced in transformed root cultures of P. cuernavacana. Hairy roots were induced by inoculation of internodal segments of sterile plants of P. cuernavacana with Agrobacterium rhizogenes AR12 strain. The axenic liquid MS medium cultures of the hairy roots isolated from the internodes showed active growth in the absence of growth regulators. The transformed nature of the tissue was confirmed by genomic integration (PCR and slot blot hybridization) and expression (enzyme activity) of the marker gus-gene. The production of perezone by a transformed root culture was evidenced by IR spectroscopy. Our results offer an alternative for enhanced production of perezone and represent an advantage over its extraction from natural plant populations which present problems in their agronomic culture.     

Effect of oleuropein and sodium chloride on viability and metabolism of Lactobacillus plantarum

 The effect of the addition of oleuropein (OLP) and NaCl on the growth and the DL-lactic acid production of Lactobacillus plantarum DSM 10492 has been investigated by using an unconventional medium. The growth of L. plantarum was not inhibited by the addition of increasing amounts of untreated OLP in the presence or absence of glucose. However, bacterial cells grew in quantity slightly with OLP alone. The increased addition of NaCl was associated with a delay in growth. Moreover, there was no growth with 8% NaCl. The addition of both NaCl and OLP resulted in growth inhibition, and the survival of cells decreased strongly. The main fermentation product was DL-lactic acid, but acetic acid was also detected after a prolonged incubation. L. plantarum produced DL-lactic acid in the presence of OLP alone but its formation decreased with increasing levels of OLP. On the other hand, heat-treated OLP had a bactericidal effect. Received: 16 October 1995/Received last revision: 5 February 1996/Accepted: 12 February 1996