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31.
Genomic analysis of the unfolded protein response in Arabidopsis shows its connection to important cellular processes 总被引:12,自引:0,他引:12
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We analyzed the breadth of the unfolded protein response (UPR) in Arabidopsis using gene expression analysis with Affymetrix GeneChips. With tunicamycin and DTT as endoplasmic reticulum (ER) stress-inducing agents, we identified sets of UPR genes that were induced or repressed by both stresses. The proteins encoded by most of the upregulated genes function as part of the secretory system and comprise chaperones, vesicle transport proteins, and ER-associated degradation proteins. Most of the downregulated genes encode extracellular proteins. Therefore, the UPR may constitute a triple effort by the cell: to improve protein folding and transport, to degrade unwanted proteins, and to allow fewer secretory proteins to enter the ER. No single consensus response element was found in the promoters of the 53 UPR upregulated genes, but half of the genes contained response elements also found in mammalian UPR regulated genes. These elements are enriched from 4.5- to 15-fold in this upregulated gene set. 相似文献
32.
Cronk KM Wilkinson GA Grimes R Wheeler EF Jhaveri S Fundin BT Silos-Santiago I Tessarollo L Reichardt LF Rice FL 《Development (Cambridge, England)》2002,129(15):3739-3750
This study demonstrates that innervation dependent on two different neurotrophin tyrosine kinase (trk) receptors can form the same types of sensory endings (Merkel endings) in the same target (Merkel cells of vibrissa follicles). Some endings transiently express trkA during their initial development, whereas others express trkC throughout their development. Consequently, elimination of kinase domains of either trkA or trkC each result in a partial loss of Merkel endings, whereas absence of kinase domains of both receptors results in a total loss. At the onset of Merkel ending development, at least one kinase-lacking trkC isoform is transiently expressed on all the follicle cells, while neurotrophin 3 is transiently expressed only in the cells at the middle third of the follicle where the Merkel endings and cells develop. This transient non-neuronal expression of truncated trkC is essential for development of any Merkel endings, whereas some Merkel endings and cells still begin to develop in the absence of neurotrophin 3. Therefore, truncated trkC plays a more important role in the development of this innervation than kinase forms of trkA or trkC or of NT3, the only known ligand for trkC receptors. 相似文献
33.
Diana Valverde Mònica Bayés Immaculada Martínez Daniel Grinberg Lluïsa Vilageliu Susana Balcells Roser Gonzàlez-Duarte Montserrat Baiget 《Human genetics》1994,94(2):193-194
Arrestin is a component of the light transduction cascade that takes place in the outer segment of retinal rods. In situ hybridization and linkage analysis have localized the arrestin gene to a region of 50 cM between CRYG and D2S23/D2S55 on chromosome 2q24–37. We have performed pairwise and multipoint linkage analysis between arrestin and four highly polymorphic markers from this region. The results indicate tight linkage between the gene and the microsatellite D2S172 (Z
max
= 9.25 at =0.038). This fine localization of the gene should provide a useful tool for cosegregation analyses involving the arrestin gene. 相似文献
34.
Roberta Cozzi Enrico Malito Maddalena Lazzarin Annalisa Nuccitelli Andrea Castagnetti Matthew J. Bottomley Immaculada Margarit Domenico Maione C. Daniela Rinaudo 《PloS one》2015,10(5)
Group B Streptococcus (GBS) is a major cause of invasive disease in infants. Like other Gram-positive bacteria, GBS uses a sortase C-catalyzed transpeptidation mechanism to generate cell surface pili from backbone and ancillary pilin precursor substrates. The three pilus types identified in GBS contain structural subunits that are highly immunogenic and are promising candidates for the development of a broadly-protective vaccine. Here we report the X-ray crystal structure of the backbone protein of pilus 2b (BP-2b) at 1.06Å resolution. The structure reveals a classical IgG-like fold typical of the pilin subunits of other Gram-positive bacteria. The crystallized portion of the protein (residues 185-468) encompasses domains D2 and D3 that together confer high stability to the protein due to the presence of an internal isopeptide bond within each domain. The D2+D3 region, lacking the N-terminal D1 domain, was as potent as the entire protein in conferring protection against GBS challenge in a well-established mouse model. By site-directed mutagenesis and complementation studies in GBS knock-out strains we identified the residues and motives essential for assembly of the BP-2b monomers into high-molecular weight complexes, thus providing new insights into pilus 2b polymerization. 相似文献
35.
Teresa Paula Cañamás Immaculada Viñas Rosario Torres Josep Usall Cristina Solsona Neus Teixidó 《Biological Control》2011,56(2):150-158
There is increasing interest in the use of biological control agents (BCAs) to replace synthetic fungicides. The aim of this study was to evaluate the effectiveness of combining different formulation strategies on the establishment, survival, persistence and efficacy of Candida sake CPA-1 applied in the field in order to control Botrytis cinerea in grapes. Different formulations of C. sake cells were applied at flowering, pea sized berries, veraison and before harvest. In 2005, heat adapted or non-adapted fresh cells of C. sake were tested. In 2006, these same treatments were tested but, in this case, the C. sake cells were also formulated in an isotonic solution. The compound Fungicover® (FC) an edible coating was evaluated as a potential additive for C. sake treatments. Spray applications of different formulations of C. sake resulted in colonisation of bunches under field conditions and when combined with FC, C. sake had significantly higher survival rates (up to 50% higher) compared with C. sake without additive FC. Formulation of C. sake cells in an isotonic solution combined with FC resulted in Botrytis spp. control similarly than fungicide treatment. The potential of C. sake for biocontrol of Botrytis bunch rot of grapevine has been demonstrated. FC has a beneficial effect on the BCA, improving the persistence of C. sake cells on the host and its efficacy to levels comparable to fungicide treatment. It is possible to broaden the spectrum of use of BCAs using different formulation strategies and to thereby develop practical uses under field conditions. 相似文献
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Jesús Aguirre‐Gutiérrez Imma Oliveras Sami Rifai Sophie Fauset Stephen Adu‐Bredu Kofi Affum‐Baffoe Timothy R. Baker Ted R. Feldpausch Agne Gvozdevaite Wannes Hubau Nathan J. B. Kraft Simon L. Lewis Sam Moore Ülo Niinemets Theresa Peprah Oliver L. Phillips Kasia Ziemińska Brian Enquist Yadvinder Malhi 《Ecology letters》2019,22(5):855-865
Climatic changes have profound effects on the distribution of biodiversity, but untangling the links between climatic change and ecosystem functioning is challenging, particularly in high diversity systems such as tropical forests. Tropical forests may also show different responses to a changing climate, with baseline climatic conditions potentially inducing differences in the strength and timing of responses to droughts. Trait‐based approaches provide an opportunity to link functional composition, ecosystem function and environmental changes. We demonstrate the power of such approaches by presenting a novel analysis of long‐term responses of different tropical forest to climatic changes along a rainfall gradient. We explore how key ecosystem's biogeochemical properties have shifted over time as a consequence of multi‐decadal drying. Notably, we find that drier tropical forests have increased their deciduous species abundance and generally changed more functionally than forests growing in wetter conditions, suggesting an enhanced ability to adapt ecologically to a drying environment. 相似文献
37.
R. M. Román‐Cuesta C. Carmona‐Moreno G. Lizcano M. New M. Silman T. Knoke Y. Malhi I. Oliveras H. Asbjornsen M. Vuille 《Global Change Biology》2014,20(6):1929-1942
Global climate models suggest enhanced warming of the tropical mid and upper troposphere, with larger temperature rise rates at higher elevations. Changes in fire activity are amongst the most significant ecological consequences of rising temperatures and changing hydrological properties in mountainous ecosystems, and there is a global evidence of increased fire activity with elevation. Whilst fire research has become popular in the tropical lowlands, much less is known of the tropical high Andean region (>2000masl, from Colombia to Bolivia). This study examines fire trends in the high Andes for three ecosystems, the Puna, the Paramo and the Yungas, for the period 1982–2006. We pose three questions: (i) is there an increased fire response with elevation? (ii) does the El Niño‐ Southern Oscillation control fire activity in this region? (iii) are the observed fire trends human driven (e.g., human practices and their effects on fuel build‐up) or climate driven? We did not find evidence of increased fire activity with elevation but, instead, a quasicyclic and synchronous fire response in Ecuador, Peru and Bolivia, suggesting the influence of high‐frequency climate forcing on fire responses on a subcontinental scale, in the high Andes. ENSO variability did not show a significant relation to fire activity for these three countries, partly because ENSO variability did not significantly relate to precipitation extremes, although it strongly did to temperature extremes. Whilst ENSO did not individually lead the observed regional fire trends, our results suggest a climate influence on fire activity, mainly through a sawtooth pattern of precipitation (increased rainfall before fire‐peak seasons (t‐1) followed by drought spells and unusual low temperatures (t0), which is particularly common where fire is carried by low fuel loads (e.g., grasslands and fine fuel). This climatic sawtooth appeared as the main driver of fire trends, above local human influences and fuel build‐up cyclicity. 相似文献
38.
Does salt stress constrain spatial distribution of dune building grasses Ammophila arenaria and Elytrichia juncea on the beach?
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Marinka E. B. van Puijenbroek Corry Teichmann Noortje Meijdam Imma Oliveras Frank Berendse Juul Limpens 《Ecology and evolution》2017,7(18):7290-7303
Rising sea levels threaten coastal safety by increasing the risk of flooding. Coastal dunes provide a natural form of coastal protection. Understanding drivers that constrain early development of dunes is necessary to assess whether dune development may keep pace with sea‐level rise. In this study, we explored to what extent salt stress experienced by dune building plant species constrains their spatial distribution at the Dutch sandy coast. We conducted a field transplantation experiment and a glasshouse experiment with two dune building grasses Ammophila arenaria and Elytrigia juncea. In the field, we measured salinity and monitored growth of transplanted grasses in four vegetation zones: (I) nonvegetated beach, (II) E. juncea occurring, (III) both species co‐occurring, and (IV) A. arenaria dominant. In the glasshouse, we subjected the two species to six soil salinity treatments, with and without salt spray. We monitored biomass, photosynthesis, leaf sodium, and nutrient concentrations over a growing season. The vegetation zones were weakly associated with summer soil salinity; zone I and II were significantly more saline than zones III and IV. Ammophila arenaria performed equally (zone II) or better (zones III, IV) than E. juncea, suggesting soil salinity did not limit species performance. Both species showed severe winter mortality. In the glasshouse, A. arenaria biomass decreased linearly with soil salinity, presumably as a result of osmotic stress. Elytrigia juncea showed a nonlinear response to soil salinity with an optimum at 0.75% soil salinity. Our findings suggest that soil salinity stress either takes place in winter, or that development of vegetated dunes is less sensitive to soil salinity than hitherto expected. 相似文献
39.