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151.
Anthropometric,Metabolic and Molecular Determinants of Human Epidermal Growth Factor Receptor 2 Expression in Luminal B Breast Cancer 下载免费PDF全文
Patrizia Vici Anna Crispo Antonio Giordano Luigi Di Lauro Francesca Sperati Irene Terrenato Laura Pizzuti Domenico Sergi Marcella Mottolese Claudio Botti Maria Grimaldi Immacolata Capasso Giuseppe D'Aiuto Maurizio Di Bonito Flaviano Di Paola Marcello Maugeri‐Saccà Maurizio Montella Maddalena Barba 《Journal of cellular physiology》2015,230(8):1708-1712
152.
M Comar N Zanotta E Croci I Murru R Marci C Pancaldi O Dolcet S Luppi M Martinelli E Giolo G Ricci M Tognon 《PloS one》2012,7(8):e42880
In recent years the incidence of male infertility has increased. Many risk factors have been taken into consideration, including viral infections. Investigations into viral agents and male infertility have mainly been focused on human papillomaviruses, while no reports have been published on polyomaviruses and male infertility. The aim of this study was to verify whether JC virus and BK virus are associated with male infertility. Matched semen and urine samples from 106 infertile males and 100 fertile males, as controls, were analyzed. Specific PCR analyses were carried out to detect and quantify large T (Tag) coding sequences of JCV and BKV. DNA sequencing, carried out in Tag JCV-positive samples, was addressed to viral protein 1 (VP1) coding sequences. The prevalence of JCV Tag sequences in semen and urine samples from infertile males was 34% (72/212), whereas the BKV prevalence was 0.94% (2/212). Specifically, JCV Tag sequences were detected in 24.5% (26/106) of semen and 43.4% (46/106) of urine samples from infertile men. In semen and urine samples from controls the prevalence was 11% and 28%, respectively. A statistically significant difference (p<0.05) in JCV prevalence was disclosed in semen and urine samples of cases vs. controls. A higher JC viral DNA load was detected in samples from infertile males than in controls. In samples from infertile males the JC virus type 2 strain, subtype 2b, was more prevalent than ubiquitous type 1. JCV type 2 strain infection has been found to be associated with male infertility. These data suggest that the JC virus should be taken into consideration as an infectious agent which is responsible for male infertility. 相似文献
153.
Guido Mastrobuoni Huili Qiao Immacolata Iovinella Simona Sagona Alberto Niccolini Francesca Boscaro Beniamino Caputo Marta R. Orejuela Alessandra della Torre Stefan Kempa Antonio Felicioli Paolo Pelosi Gloriano Moneti Francesca Romana Dani 《PloS one》2013,8(11)
Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are small soluble polypeptides that bind semiochemicals in the lymph of insect chemosensilla. In the genome of Anopheles gambiae, 66 genes encode OBPs and 8 encode CSPs. Here we monitored their expression through classical proteomics (2D gel-MS analysis) and a shotgun approach. The latter method proved much more sensitive and therefore more suitable for tiny biological samples as mosquitoes antennae and eggs. Females express a larger number and higher quantities of OBPs in their antennae than males (24 vs 19). OBP9 is the most abundant in the antennae of both sexes, as well as in larvae, pupae and eggs. Of the 8 CSPs, 4 were detected in antennae, while SAP3 was the only one expressed in larvae. Our proteomic results are in fairly good agreement with data of RNA expression reported in the literature, except for OBP4 and OBP5, that we could not identify in our analysis, nor could we detect in Western Blot experiments. The relatively limited number of soluble olfactory proteins expressed at relatively high levels in mosquitoes makes further studies on the coding of chemical messages at the OBP level more accessible, providing for few specific targets. Identification of such proteins in Anopheles gambiae might facilitate future studies on host finding behavior in this important disease vector. 相似文献
154.
Daniela I. Abbrescia Lucia Catucci Lucia D'Accolti Nunzio Denora Immacolata Fanizza Caterina Fusco Gianluigi La Piana 《Journal of peptide science》2013,19(12):751-757
Valinomycin (VLM, 1) is a K+ ionophore cyclodepsipeptide capable of depolarizing mitochondria and inducing apoptosis to several mammalian cell types, including a number of tumor cell lines. With the aim of creating VLM‐based ligand‐targeted anticancer drugs that may selectively convey VLM to pathological cells, we have previously introduced derivatizable hydroxyl handles into the VLM structure, allowing to access a three‐entity library of monohydroxyl VLMs (HyVLMs) bearing the OH group at the isopropyl side chain of a d ‐Hyi, d ‐Val, or l ‐Val residue (analogs 2–4, respectively). Herein, the levels of bioactivity retained by the conjugable HyVLMs have been assessed on the basis of their ability to alter the functionality of isolated rat‐liver mitochondria. Experiments run with HyVLMs in the range 1–10 nM and in 20 or 125 mM KCl medium show that the hydroxyl group reduces the potency of HyVLMs relative to VLM to an extent that depends upon the molecular site involved in the hydroxylation. On the other hand, estimation of the stability constants of complexes (in methanol at 25 °C) of each analog with Na+, K+, and Cs+ reveals that HyVLMs nicely retain the VLM binding features, except for a moderate increase in the stability of Na+ complexes. These findings, along with pertinent structural considerations, suggest that the incorporation of OH into the VLM structure might actually have altered its K+ transporting ability across mitochondrial membranes. Besides facing new aspects of VLM structure–activity relationship, these studies set the basis for the rational design of ligand‐HyVLMs conjugates through derivatization of hanging OH group. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
155.
Prof. Francesco Galdiero Caterina Romano Carratelli Concetta Bentivoglio Immacolata Nuzzo Marilena Galdiero 《Current microbiology》1991,22(3):195-197
This study has assessed the changes in the polarization fluorescence of 1,6-diphenyl-1,3,5-hexatriene in the splenic membrane of mice treated with clindamycin, penicillin, cefoxitin, cefamandole, cephaloridine, tetracycline, and erythromycin. The data show that most of the antibiotics tested modified membrane activity, as was shown by the changes in membrane microviscosity parameters. 相似文献
156.
Davide A Cecchini Immacolata Serra Daniela Ubiali Marco Terreni Alessandra M Albertini 《BMC biotechnology》2007,7(1):54