全文获取类型
收费全文 | 2617篇 |
免费 | 176篇 |
国内免费 | 1篇 |
专业分类
2794篇 |
出版年
2022年 | 14篇 |
2021年 | 36篇 |
2020年 | 17篇 |
2019年 | 16篇 |
2018年 | 24篇 |
2017年 | 27篇 |
2016年 | 46篇 |
2015年 | 62篇 |
2014年 | 85篇 |
2013年 | 125篇 |
2012年 | 156篇 |
2011年 | 142篇 |
2010年 | 108篇 |
2009年 | 95篇 |
2008年 | 136篇 |
2007年 | 162篇 |
2006年 | 133篇 |
2005年 | 134篇 |
2004年 | 132篇 |
2003年 | 131篇 |
2002年 | 120篇 |
2001年 | 62篇 |
2000年 | 53篇 |
1999年 | 72篇 |
1998年 | 29篇 |
1997年 | 35篇 |
1996年 | 35篇 |
1995年 | 28篇 |
1994年 | 25篇 |
1993年 | 17篇 |
1992年 | 50篇 |
1991年 | 41篇 |
1990年 | 53篇 |
1989年 | 48篇 |
1988年 | 32篇 |
1987年 | 33篇 |
1986年 | 33篇 |
1985年 | 28篇 |
1984年 | 22篇 |
1983年 | 20篇 |
1982年 | 32篇 |
1981年 | 23篇 |
1980年 | 17篇 |
1979年 | 17篇 |
1978年 | 8篇 |
1977年 | 11篇 |
1975年 | 12篇 |
1973年 | 10篇 |
1969年 | 8篇 |
1968年 | 9篇 |
排序方式: 共有2794条查询结果,搜索用时 15 毫秒
61.
E Benedikt A Gossauer H P K?st W Miki K Yamaguchi 《European journal of biochemistry》1988,175(3):643-648
The ovaries of the marine snail Turbo cornutus contain a number of pigments. So far, the presence of carotenoids and a chromoprotein with a bile pigment, called turboverdin (= 3(2)-hydroxy-mesobiliverdin IX alpha), as its prosthetic group are known. The present work describes the isolation and structure elucidation of two further bile pigments, biliverdin IX delta and neobiliverdin IX delta. This is the first report of naturally occurring bile pigments with IX delta structure. 相似文献
62.
Feedbacks between nutrient cycling and vegetation predict plant species coexistence and invasion 总被引:5,自引:0,他引:5
To investigate the role of species‐specific litter decomposability in determining plant community structure, we constructed a theoretical model of the codevelopmental dynamics of soil and vegetation. This model incorporates feedback between vegetation and soil. Vegetation changes the nutrient conditions of soil by affecting mineralization processes; soil, in turn, has an impact on plant community structure. The model shows that species‐level traits (decomposability, reproductive and competitive abilities) determine whether litter feedback effects are positive or negative. The feedback determines community‐level properties, such as species composition and community stability against invasion. The model predicts that positive feedback may generate multiple alternative steady states of the plant community, which differ in species richness or community composition. In such cases, the realized state is determined by initial abundance of co‐occurring species. Further, the model shows that the importance of species‐level traits depends on environmental conditions such as system fertility. 相似文献
63.
Electron-microscopic studies were made on the appearance of synapses in the intramural ganglion (Auerbach) and findings were correlated with the onset and development of intestinal peristalsis in 6- to 30-week-old human and rabbit fetuses from the 12th day after conception until birth. At stage I, in which the small intestine shows no indication of a muscle layer or spontaneous peristalsis, primitive synapses containing several clear vesicles and a few cored vesicles are seen on neuroblasts and their processes (dendrites). At stage II, in which the circular muscle is developed and bidirectional peristalsis occurs, synaptic profiles can be classified into 3 types. Type 1 is the most numerous but seldom shows membrane specificity on the synaptic portion. Types 2 and 3 have small flattened vesicles and small round vesicles, respectively. They are further characterized by thickening of snyaptic membranes and aggregation of small clear vesicles associated with the presynaptic membrane. At stage III, the longitudinal muscle layer develops in the small intestine. At this stage, nerve terminals containing mainly cored vesicles have been observed and classified into types 4 and 5, according to their morphology. At stage IV, antiperistalsis no longer occurs and type 6 nerve terminals in the intramural ganglia can be recognized by their densely packed, large-cored vesicles. The possible physiological significance of the nerve terminals has been discussed. 相似文献
64.
Dubouzet JG Maeda S Sugano S Ohtake M Hayashi N Ichikawa T Kondou Y Kuroda H Horii Y Matsui M Oda K Hirochika H Takatsuji H Mori M 《Plant biotechnology journal》2011,9(4):466-485
Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determined in 72 lines that showed consistent resistance after three independent screens. Pst DC3000 resistance was verified for 19 genes by characterizing other independent Arabidopsis lines for the same genes in the original rice-FOX hunting population or obtained by reintroducing the genes into ecotype Columbia by floral dip transformation. Thirteen lines of these 72 selections were also resistant to the fungal pathogen Colletotrichum higginsianum. Eight genes that conferred resistance to Pst DC3000 in Arabidopsis have been introduced into rice for overexpression, and transformants were evaluated for resistance to the rice bacterial pathogen, Xanthomonas oryzae pv. oryzae. One of the transgenic rice lines was highly resistant to Xanthomonas oryzae pv. oryzae. Interestingly, this line also showed remarkably high resistance to Magnaporthe grisea, the fungal pathogen causing rice blast, which is the most devastating rice disease in many countries. The causal rice gene, encoding a putative receptor-like cytoplasmic kinase, was therefore designated as BROAD-SPECTRUM RESISTANCE 1. Our results demonstrate the utility of the rice-FOX Arabidopsis lines as a tool for the identification of genes involved in plant defence and suggest the presence of a defence mechanism common between monocots and dicots. 相似文献
65.
Electron transport chain from glycerol 3-phosphate to nitrate in Escherichia coli. 总被引:4,自引:1,他引:4 下载免费PDF全文
It is known that in Escherichia coli two dehydrogenases of the flavoprotein kind can participate in the transfer of hydrogens from sn-glycerol 3-phosphate (G3P) to nitrate and that possession of either enzyme is sufficient to permit anaerobic growth on glycerol as carbon source and nitrate as hydrogen acceptor. Results from this study show that under such a growth condition a protein with light-absorption characteristics of cytochrome b1 is induced. If G3P, nitrate, and adenosine diphosphate are added anaerobically to a particulate fraction prepared from these cells, four reactions can be detected: (i) the reduction of the cytochrome b1-like protein, (ii) the formation of dihydroxyacetone phosphate (DHAP), (iii) the formation of nitrite, and (iv) the generation of adenosine 5'-triphosphate (ATP). The anaerobic G3P dehydrogenase system can yield an ATP-DHAP (or ATP-nitrite) molar ratio of about 0.2, whereas the aerobic G3P dehydrogenase system can yield a corresponding ratio of about 0.3. The hydrogen transfer activity is sensitive to respiratory inhibitors such as cyanide, Rotenone, and 2-heptyl-4-hydroxyquinoline-N-oxide. 相似文献
66.
The interconversion of chlorophyll a and chlorophyll b, referred to as the chlorophyll cycle, plays a crucial role in the processes of greening, acclimation to light intensity, and senescence. The chlorophyll cycle consists of three reactions: the conversions of chlorophyll a to chlorophyll b by chlorophyllide a oxygenase, chlorophyll b to 7-hydroxymethyl chlorophyll a by chlorophyll b reductase, and 7-hydroxymethyl chlorophyll a to chlorophyll a by 7-hydroxymethyl chlorophyll a reductase. We identified 7-hydroxymethyl chlorophyll a reductase, which is the last remaining unidentified enzyme of the chlorophyll cycle, from Arabidopsis thaliana by genetic and biochemical methods. Recombinant 7-hydroxymethyl chlorophyll a reductase converted 7-hydroxymethyl chlorophyll a to chlorophyll a using ferredoxin. Both sequence and biochemical analyses showed that 7-hydroxymethyl chlorophyll a reductase contains flavin adenine dinucleotide and an iron-sulfur center. In addition, a phylogenetic analysis elucidated the evolution of 7-hydroxymethyl chlorophyll a reductase from divinyl chlorophyllide vinyl reductase. A mutant lacking 7-hydroxymethyl chlorophyll a reductase was found to accumulate 7-hydroxymethyl chlorophyll a and pheophorbide a. Furthermore, this accumulation of pheophorbide a in the mutant was rescued by the inactivation of the chlorophyll b reductase gene. The downregulation of pheophorbide a oxygenase activity is discussed in relation to 7-hydroxymethyl chlorophyll a accumulation. 相似文献
67.
Riki Kawaguchi Ming Zhong Miki Kassai Mariam Ter-Stepanian Hui Sun 《The Journal of membrane biology》2013,246(8):647-660
Retinoids are vitamin A derivatives with diverse biological functions. Both natural and artificial retinoids have been used as therapeutic reagents to treat human diseases, but not all retinoid actions are understood mechanistically. Plasma retinol binding protein (RBP) is the principal and specific carrier of vitamin A in the blood. STRA6 is the membrane receptor for RBP that mediates cellular vitamin A uptake. The effects of retinoids or related compounds on the receptor’s vitamin A uptake activity and its catalytic activities are not well understood. In this study, we dissected the membrane receptor-mediated vitamin A uptake mechanism using various retinoids. We show that a subset of retinoids strongly stimulates STRA6-mediated vitamin A release from holo-RBP. STRA6 also catalyzes the exchange of retinol in RBP with certain retinoids. The effect of retinoids on STRA6 is highly isomer-specific. This study provides unique insights into the RBP receptor’s mechanism and reveals that the vitamin A transport machinery can be a target of retinoid-based drugs. 相似文献
68.
T Suzuki M Murakami N Onai E Fukuda Y Hashimoto M H Sonobe T Kameda M Ichinose K Miki H Iba 《Journal of virology》1994,68(6):3527-3535
69.
Expression of the BnmNAP subfamily of napin genes coincides with the induction of Brassica microspore embryogenesis 总被引:3,自引:0,他引:3
Kim A. Boutilier María-Jesús Ginés Janice M. DeMoor Bin Huang Chris L. Baszczynski V. N. Iyer Brian L. Miki 《Plant molecular biology》1994,26(6):1711-1723
Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. We show that this switch in developmental pathways is accompanied by the induction of high levels of napin seed storage protein gene expression. Changes in the plant growth or microspore culture conditions were not by themselves sufficient to induce napin gene expression. Specific members of the napin multigene family were cloned from a cDNA library prepared from microspores that had been induced to undergo embryogenesis. The majority of napin clones represented three members (BnmNAP2, BnmNAP3 and BnmNAP4) that, along with a previously isolated napin genomic clone (BngNAP1), constitute the highly conserved BnmNAP subfamily of napin genes. Both RNA gel blot analysis, using a subfamily-specific probe, and histochemical analysis of transgenic plants expressing a BngNAP1 promoter--glucuronidase gene fusion demonstrated that the BnmNAP subfamily is expressed in embryogenic microspores as well as during subsequent stages of microsporic embryo development. 相似文献
70.