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91.
A. J. McMichael Jennifer M. Phillips A. R. Williamson Thereza Imanishi O. Mäkelä 《Immunogenetics》1975,2(1):161-173
The primary anti-NP response in C57BL/6 and CBA mice was analyzed by isoelectric focusing. Antibody responses in C57 mice were restricted in heterogeneity and over 80 % of this strain shared an isoelectric focusing spectrum characteristic of an homogeneous antibody (spectrotype). This spectrotype N-l was inherited as a dominant characteristic in (CBA×C57)F1 mice, and backcross analysis revealed that it was genetically linked to the heavy chain allotypeIg-1
b
. It thus behaved as a marker similar to the fine specificity characteristic of heteroclitic antibody. Elution studies showed that antibody with the spectrotype N-1 was heteroclitic, with a higher affinity for NIP and NNP than for the immunogen NP. It is argued that a) a single germ-lineV gene (designated N-1) codes for the VH region of this antibody and b) in C57BL/6 mice this geneV
H
N-1 is closely linked toIg-1
b
.Abbreviations BSA
bovine serum albumin
- CG
Chicken globulin
- NP
(4-Hydroxy-3-nitrophenyl)acetyl
- NIP
(4-Hydroxy-5-iodo-3-nitrophenyl)acetyl
- NBrP
(4 Hydroxy-5-bromo-3-nitrophenyl)acetyl
- NNP
(4-Hydroxy-3,5-dinitrophenyl)acetyl
- NIP-Cap
NIP-aminocaproate
- I50
Hapten concentration causing 50% inhibition of antigen binding
- SRC
Sheep red cells
- IEF
Isoelectric focusing
- PBS
Phosphate buffered saline, pH 7.4 相似文献
92.
Cyclic peptides containing sarcosine, cyclo-(Pro-Sar-Gly)2, cyclo-(Sar-Sar-Gly)2, cyclo-(Sar4), and cyclo-(Sar6) have been synthesized by the cyclization of the p-nitrophenyl ester of linear peptides. The tert-butoxycarbonyl group was used as the Nα-protecting group, which was removed by acid. Benzyl ester was used to protect the C-terminal. tert-butoxycarbonylpeptide was obtained by the stepwise elongation of the peptide bond by the carbodiimide method. Deblocking and cyclization of the linear peptides gave the cyclic peptides. 相似文献
93.
94.
Y F Sasaki H Imanishi M Watanabe A Sekiguchi M Moriya Y Shirasu K Tutikawa 《Mutation research》1986,174(2):145-147
The spot test with 1,2-dibromo-3-chloropropane (DBCP) was carried out using male PW and female C57BL/6 mice. DBCP induced recessive colour spots in offspring with a significantly high frequency of 2.9%, showing that this chemical is mutagenic for somatic cells of mice in vivo. 相似文献
95.
Interferon: a mediator of indoleamine 2,3-dioxygenase induction by lipopolysaccharide, poly(I) X poly(C), and pokeweed mitogen in mouse lung 总被引:2,自引:0,他引:2
R Yoshida T Oku J Imanishi T Kishida O Hayaishi 《Archives of biochemistry and biophysics》1986,249(2):596-604
When C3H/He mice were treated with lipopolysaccharide, poly(I) X poly(C), or pokeweed mitogen, the serum interferon titer increased almost instantaneously (100-2000 units/ml), and then the pulmonary indoleamine 2,3-dioxygenase was induced 50- to 140-fold. The peaks corresponding to interferon induction always preceded (approximately 24 h) those corresponding to dioxygenase induction. In C3H/HeJ (lipopolysaccharide-nonresponder) mice, however, lipopolysaccharide was totally inert in induction of both interferon and dioxygenase, although treatment with poly(I) X poly(C) and pokeweed mitogen led to a remarkable increase in the serum interferon titer and the enzyme activity. When lymphocytes of C3H/HeJ mice were inactivated by X irradiation and then reconstituted by the transfer of spleen cells from C3H/He mice, both enzyme and interferon from C3H/HeJ mice thus treated were induced almost normally after the lipopolysaccharide treatment. In addition, murine interferon alpha/beta, which was injected intravenously in C3H/He or C3H/HeJ mice, almost instantaneously and dose-dependently induced the pulmonary enzyme, and at a dose of 10(5) units per mouse the enzyme activity was enhanced 20- to 26-fold in these two strains of mice. These results suggest that interferon, which is generated by the interaction of lymphocytes with lipopolysaccharide, poly(I) X poly(C), or pokeweed mitogen, is a mediator of indoleamine 2,3-dioxygenase induction in the mouse lung by these agents. 相似文献
96.
J Imanishi M Kita T Kishida A Matsuo 《Comptes rendus des séances de la Société de biologie et de ses filiales》1980,173(6):1050-1054
Human leukocyte interferon (HL-IF) enhanced the growth inhibition of tumor cells by the human peripheral leukocytes. There was a dose relation between the enhancement of the growth inhibition of tumor cells and the antiviral activity of interferon. When the ratio of lymphocyte to tumor cell was 10:1 or 50:1, it was recognized that HL-IF enhanced the growth inhibition of tumor cells by lymphocyte. The heterologous IFs--mouse and rabbit IFs--or heat-inactivated or trypsinized IF did not enhance the growth inhibition of tumor cells by lymphocytes. RNase treatment did not reduce the antiviral activity and the growth inhibition. 相似文献
97.
Monte Carlo conformational calculations for oligosarcosines having terminal electron-donor (D) and -acceptor (A) chromophores [A-(Sar)n-D, n = 2–8] were carried out. The bulkiness of the terminal chromophores, the conformational energies, and the charge-transfer (CT) interaction energy were taken into account in the calculation. The equilibrium constant for the intramolecular end-to-end CT interaction was evaluated, and the result was compared with the experimental data. The alternating chain-length dependence of the equilibrium constant observed experimentally was reproduced by the simulation and the solvent dependence of the equilibrium constant was also reasonably explained. 相似文献
98.
99.
100.