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121.
Iman M. Ahmad Ebtihal H. Mustafa Noor H. Mustafa Lubna H. Tahtamouni Maher Y. Abdalla 《Central European Journal of Biology》2010,5(6):739-748
2DG causes cytotoxicity in cancer cells by disrupting thiol metabolism while Doxorubicin (DOX) induces cytotoxicity in tumor
cells by generating reactive oxygen species (ROS). Here we examined the combined cytotoxic action of 2DG and DOX in rapidly
dividing T47D breast cancer cells vs. slowly growing MCF-7 breast cancer cells. T47D cells exposed to the combination of 2DG/DOX
significantly decreased cell survival compared to controls, while 2DG/DOX had no effect on MCF-7 cells. 2DG/DOX also disrupted
the oxidant status of T47D treated cells, decreased intracellular total glutathione and increased glutathione disulfide (%GSSG)
compared to MCF-7 cells. Lipid peroxidation increased in T47D cells treated with 2DG and/or DOX, but not in MCF-7 cells. T47D
cells were significantly protected by NAC, indicating that the combined treatment exerts its action by increasing ROS production
and disrupting antioxidant stores. When we inhibited glutathione synthesis with BSO, T47D cells became more sensitive to 2DG/DOX-induced
cytotoxicity, but NAC significantly reversed this cytotoxic effect. Finally, 2DG/DOX, and BSO significantly increased the
%GSSG in T47D cells, an effect which was also reversed by NAC. Our results suggest that exposure of rapidly dividing breast
cancer cells to 2DG/DOX enhances cytotoxicity via oxidative stress and via disruptions to thiol metabolism. 相似文献
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Chouikha I Bree A Moulin-Schouleur M Gilot P Germon P 《Microbes and infection / Institut Pasteur》2008,10(4):432-438
Extraintestinal pathogenic Escherichia coli strains are responsible for a number of infections in humans and animals. Several ExPEC virulence genes have already been described such as iutA involved in iron acquisition and ibeA required for invasion of eukaryotic cells. In this study we used the chicken model to study the expression of iutA and ibeA by two ExPEC strains during growth of bacteria in LB medium and during the infection. Expression of iutA and ibeA were shown to be higher in stationary phase than in exponential phase in vitro. During infection, iutA expression was increased at least 50-fold in the airsac and in the lung 3, 6 and 24h. p.i. compared to in vitro grown bacteria. Expression of ibeA was increased 2.5-9-fold in the airsac in the early stages of the infection only. This is the first report analyzing quantitatively the expression of ExPEC virulence genes during the course of the infection. The model described could be useful to study the expression of other ExPEC virulence genes. 相似文献
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Shawky Samir A. El-Borai Mohamed H. Khaled Hussein M. Guda Iman Mohanad Marwa Abdellateif Mona S. Zekri Abdel-Rahman N. Bahanasy Abeer A. 《Molecular biology reports》2019,46(4):4063-4076
Molecular Biology Reports - Diffuse Large B-cell lymphoma (DLBCL) is an aggressive disease with heterogeneous outcome and marked variable response to chemotherapy. We assessed promoter... 相似文献
128.
Towards multidimensional genome annotation 总被引:1,自引:0,他引:1
Our information about the gene content of organisms continues to grow as more genomes are sequenced and gene products are characterized. Sequence-based annotation efforts have led to a list of cellular components, which can be thought of as a one-dimensional annotation. With growing information about component interactions, facilitated by the advancement of various high-throughput technologies, systemic, or two-dimensional, annotations can be generated. Knowledge about the physical arrangement of chromosomes will lead to a three-dimensional spatial annotation of the genome and a fourth dimension of annotation will arise from the study of changes in genome sequences that occur during adaptive evolution. Here we discuss all four levels of genome annotation, with specific emphasis on two-dimensional annotation methods. 相似文献
129.
Taiki Umezawa Nurcahyo Iman Prakoso Miho Kannaka Yasuyuki Nogata Erina Yoshimura Tatsufumi Okino Fuyuhiko Matsuda 《化学与生物多样性》2019,16(1)
Omaezallene derivatives (nor‐bromoallene, nor‐bromodiene, and bromoenynes) were successfully synthesized. Their antifouling activity and toxicity to the cypris larvae of the barnacle Amphibalanus amphitrite and ecotoxicity to the marine crustacean Tigriopus japonicus were studied. It was revealed that the two side chains of omaezallene were essential to its antifouling activity because the activities of nor‐bromoallene and nor‐bromodiene were significantly diminished. The bromoenyne was found to exhibit potent antifouling activities comparable to omaezallene with low toxicity and ecotoxicity. Preparation of bromoenyne framework is much easier than that of bromodiene moiety in omaezallene. Based on the antifouling activities of the bromoenynes, the synthesis of fluorescent probes and evaluation of their biological activities were also carried out. 相似文献
130.
C. Raina MacIntyre Iman Ridda Zhanhai Gao Aye M. Moa Peter B. McIntyre John S. Sullivan Thomas R. Jones Andrew Hayen Richard I. Lindley 《PloS one》2014,9(4)