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111.
Kenji Maekaji Mitsuo Sunagawa Hiroshi Imai 《Bioscience, biotechnology, and biochemistry》2013,77(3):165-169
Two kinds of anthocyanin were isolated from the petals of white peach and examined by paper chromatographic and spectral analyses. The results showed that one of them is crysanthemin and the other is a derivative of chrysanthemin which has not been isolated from peaches previously. 相似文献
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Purified malate-vitamin K reductase (MKR) from , which requires phospholipid and FAD for the enzymatic reduction of dye electron acceptors, was used as an electron transfer system in combination with synthetic phospholipid vesicles. Ferricyanide entrapped inside the sonically prepared vesicles was enzymatically reduced by MKR. The rate of reduction of ferricyanide by MKR was dependent upon a lipid soluble mediator. The rate of enzymatic reduction of ferricyanide by malate-vitamin K reductase was increased by the addition of phenazine methosulfate (PMS) or benzoquinone. 相似文献
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Tsuchiya Kenji Tomioka Noriko Sano Tomoharu Kohzu Ayato Komatsu Kazuhiro Imai Akio Hayakawa Kazuhide Nagata Takamaru Okamoto Takahiro Hirose Yoshinori 《Limnology》2020,21(1):87-96
Limnology - In Lake Biwa, the largest lake in Japan, external pollutant loads have decreased since the 1980s, leading to improved water quality, such as reduction in biochemical oxygen demand (BOD)... 相似文献
118.
Toshihiro Kimura Satoshi Fukushima Etsuko Okada Haruka Kuriyama Hisashi Kanemaru Mina Kadohisa‐Tsuruta Yosuke Kubo Satoshi Nakahara Aki Tokuzumi Ikko Kajihara Katsunari Makino Azusa Miyashita Jun Aoi Takamitsu Makino Hirotake Tsukamoto Yasuharu Nishimura Takashi Inozume Rong Zhang Yasushi Uemura Satoru Senju Hironobu Ihn 《Pigment cell & melanoma research》2020,33(5):744-755
Immune checkpoint inhibitors improved the survival rate of patients with unresectable melanoma. However, some patients do not respond, and variable immune‐related adverse events have been reported. Therefore, more effective and antigen‐specific immune therapies are urgently needed. We previously reported the efficacy of an immune cell therapy with immortalized myeloid cells derived from induced pluripotent stem cells (iPS‐ML). In this study, we generated OX40L‐overexpressing iPS‐ML (iPS‐ML‐Zsgreen‐OX40L) and investigated their characteristics and in vivo efficacy against mouse melanoma. We found that iPS‐ML‐Zsgreen‐OX40L suppressed the progression of B16‐BL6 melanoma, and prolonged survival of mice with ovalbumin (OVA)‐expressing B16 melanoma (MO4). The number of antigen‐specific CD8+ T cells was higher in spleen cells treated with OVA peptide‐pulsed iPS‐ML‐Zsgreen‐OX40L than in those without OX40L. The OVA peptide‐pulsed iPS‐ML‐Zsgreen‐OX40L significantly increased the number of tumor‐infiltrating T lymphocytes (TILs) in MO4 tumor. Flow cytometry showed decreased regulatory T cells but increased effector and effector memory T cells among the TILs. Although we plan to use allogeneic iPS‐ML in the clinical applications, iPS‐ML showed the tumorgenicity in the syngeneic mice model. Incorporating the suicide gene is necessary to ensure the safety in the future study. Collectively, these results indicate that iPS‐ML‐Zsgreen‐OX40L therapy might be a new method for antigen‐specific cancer immunotherapy. 相似文献
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Yohei Kurio Yosuke Koike Yu Kanesaki Satoru Watanabe Shigeki Ehira 《Molecular microbiology》2020,114(4):553-562
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Kanno Yosuke Shu En Niwa Hirofumi Seishima Mariko Ozaki Kei-ichi 《Molecular biology reports》2021,48(4):3431-3437
Molecular Biology Reports - Systemic sclerosis (SSc) is characterized by peripheral circulatory disturbance and fibrosis in skin and visceral organs. We recently demonstrated that... 相似文献