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71.
72.
Allozyme electrophoresis of four sibling parthenogenetic Caucasian rock lizards Darevskia unisexualis, D.uzzelli, D.sapphirina, and D.bendimahiensis found seven clones and five variable loci. The data supported the hypothesis that D.raddei and D.valentini are the parental species of all four parthenogens. Variation patterns in Darevskia were summarized. Species that originated from a single F1 typically consisted of one widespread clone with a few rare clones. Species with multiple origins displayed variation only
slightly higher than species with a single origin. This is contrary to other genera of parthenogenetic lizards, in which cases
massive clonal variations were observed.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
73.
A M Komarov 《Cellular and molecular biology, including cyto-enzymology》2000,46(8):1329-1336
The report describes a method for tracing nitric oxide (NO) distribution in endotoxin-treated mice using in vivo low-frequency L-band (1.1 GHz) electron spin resonance spectroscopy (ESR) in combination with extracellular nitric oxide trapping complex consisting of N-methyl-D-glucamine dithiocarbamate and iron (MGD-Fe). An ESR signal characteristic of the MGD-Fe-NO complex was found in the upper abdomen (liver region), lower abdomen and head region of ICR mice. The origin of NO from the L-arginine-NO synthase (NOS) pathway was confirmed using the NOS inhibitor N(G)-monomethyl-L-arginine (NMMA) and isotopic tracing experiments with 15N-labelled L-arginine. Experiments with mice lacking inducible NOS (iNOS) and matched wild type animals were performed using the NO trapping agent diethyldithiocarbamate (DETC). These experiments demonstrated that endotoxin-induced NO generation in the liver tissue of mice occurs via the iNOS isoform of NOS. The described in vivo ESR technique using a "whole body" resonator allows in vivo on-line detection of endogenous NO in mice. 相似文献
74.
Chelation by histidine inhibits the vacuolar sequestration of nickel in roots of the hyperaccumulator Thlaspi caerulescens 总被引:2,自引:0,他引:2
75.
Daniel I. Rubenstein Siva R. Sundaresan Ilya R. Fischhoff Chayant Tantipathananandh Tanya Y. Berger-Wolf 《PloS one》2015,10(10)
Understanding why animal societies take on the form that they do has benefited from insights gained by applying social network analysis to patterns of individual associations. Such analyses typically aggregate data over long time periods even though most selective forces that shape sociality have strong temporal elements. By explicitly incorporating the temporal signal in social interaction data we re-examine the network dynamics of the social systems of the evolutionarily closely-related Grevy’s zebras and wild asses that show broadly similar social organizations. By identifying dynamic communities, previously hidden differences emerge: Grevy’s zebras show more modularity than wild asses and in wild asses most communities consist of solitary individuals; and in Grevy’s zebras, lactating females show a greater propensity to switch communities than non-lactating females and males. Both patterns were missed by static network analyses and in general, adding a temporal dimension provides insights into differences associated with the size and persistence of communities as well as the frequency and synchrony of their formation. Dynamic network analysis provides insights into the functional significance of these social differences and highlights the way dynamic community analysis can be applied to other species. 相似文献
76.
Ivan N. Bolotov Yulia V. Bespalaya Ilya V. Vikhrev Olga V. Aksenova Paul E. Aspholm Mikhail Y. Gofarov Olga K. Klishko Yulia S. Kolosova Alexander V. Kondakov Artyom A. Lyubas Inga S. Paltser Ekaterina S. Konopleva Sakboworn Tumpeesuwan Nikita I. Bolotov Irina S. Voroshilova 《PloS one》2015,10(5)
The freshwater pearl mussel family Margaritiferidae includes 13 extant species, which are all listed by IUCN as endangered or vulnerable taxa. In this study, an extensive spatial sampling of Margaritifera spp. across the Russian Far East (Amur Basin, Kamchatka Peninsula, Kurile Archipelago and Sakhalin Island) was conducted for a revision of their taxonomy and distribution ranges. Based on their DNA sequences, shell and soft tissue morphology, three valid species were identified: Margaritifera dahurica (Middendorff, 1850), M. laevis (Haas, 1910) and M. middendorffi (Rosén, 1926). M. dahurica ranges across the Amur basin and some of the nearest river systems. M. laevis is distributed in Japan, Sakhalin Island and the Kurile Archipelago. M. middendorffi was previously considered an endemic species of the Kamchatka. However, it is widespread in the rivers of Kamchatka, Sakhalin Island, the Kurile Islands (across the Bussol Strait, which is the most significant biogeographical boundary within the archipelago), and, likely, in Japan. The Japanese species M. togakushiensis Kondo & Kobayashi, 2005 seems to be conspecific with M. middendorffi because of similar morphological patterns, small shell size (<100 mm long) and overlapped ranges, but it is in need of a separate revision. Phylogenetic analysis reveals that two NW Pacific margaritiferid species, M. laevis and M. middendorffi, formed a monophyletic 18S rDNA clade together with the North American species M. marrianae and M. falcata. The patterns that were found in these Margaritifera spp. are similar to those of freshwater fishes, indicating multiple colonizations of Eastern Asia by different mitochondrial lineages, including an ancient Beringian exchange between freshwater faunas across the Pacific. 相似文献
77.
Leonid A. Parunov Natalia P. Soshitova Mikhail V. Ovanesov Mikhail A. Panteleev Ilya I. Serebriyskiy 《Birth defects research. Part C, Embryo today : reviews》2015,105(3):167-184
This review is focused on the epidemiology of venous thromboembolism (VTE), including deep vein thrombosis (DVT) and pulmonary embolism (PE), associated with pregnancy. Superficial vein thrombosis, a less hazardous and less studied type of thrombosis in pregnant women, is beyond the scope of this review. This study discusses the VTE incidence rate in women from developed countries for both antepartum and postpartum periods and for subpopulations of women affected by additional risk factors, such as thrombophilias, circulatory diseases, preeclampsia of varying degrees of severity, and Caesarean section. To minimize bias due to historical changes in medical and obstetric practices, lifestyle, diet, etc., this review is generally limited to relatively recent studies, i.e., those that cover the last 35 years. The absolute risk or incidence rate was used to ascertain risk of VTE associated with pregnancy. For the studies where the direct incidence rates of VTE were not reported, we calculated an estimate of the observed but not reported absolute incidence rates using the data presented in respective articles. Birth Defects Research (Part C) 105:167–184, 2015. © 2015 Wiley Periodicals, Inc. 相似文献
78.
The cultivation of grapevines in the UK and many other cool climate regions is expected to benefit from the higher growing season temperatures predicted under future climate scenarios. Yet the effects of climate change on the risk of adverse weather conditions or events at key stages of crop development are not always captured by aggregated measures of seasonal or yearly climates, or by downscaling techniques that assume climate variability will remain unchanged under future scenarios. Using fine resolution projections of future climate scenarios for south-west England and grapevine phenology models we explore how risks to cool-climate vineyard harvests vary under future climate conditions. Results indicate that the risk of adverse conditions during flowering declines under all future climate scenarios. In contrast, the risk of late spring frosts increases under many future climate projections due to advancement in the timing of budbreak. Estimates of frost risk, however, were highly sensitive to the choice of phenology model, and future frost exposure declined when budbreak was calculated using models that included a winter chill requirement for dormancy break. The lack of robust phenological models is a major source of uncertainty concerning the impacts of future climate change on the development of cool-climate viticulture in historically marginal climatic regions. 相似文献
79.
In-Kyu Yoon Maria Theresa Alera Catherine B. Lago Ilya A. Tac-An Daisy Villa Stefan Fernandez Butsaya Thaisomboonsuk Chonticha Klungthong Jens W. Levy John Mark Velasco Vito G. Roque Jr. Henrik Salje Louis R. Macareo Laura L. Hermann Ananda Nisalak Anon Srikiatkhachorn 《PLoS neglected tropical diseases》2015,9(5)
Background
Chikungunya virus (CHIKV) is a globally re-emerging arbovirus for which previous studies have indicated the majority of infections result in symptomatic febrile illness. We sought to characterize the proportion of subclinical and symptomatic CHIKV infections in a prospective cohort study in a country with known CHIKV circulation.Methods/Findings
A prospective longitudinal cohort of subjects ≥6 months old underwent community-based active surveillance for acute febrile illness in Cebu City, Philippines from 2012-13. Subjects with fever history were clinically evaluated at acute, 2, 5, and 8 day visits, and at a 3-week convalescent visit. Blood was collected at the acute and 3-week convalescent visits. Symptomatic CHIKV infections were identified by positive CHIKV PCR in acute blood samples and/or CHIKV IgM/IgG ELISA seroconversion in paired acute/convalescent samples. Enrollment and 12-month blood samples underwent plaque reduction neutralization test (PRNT) using CHIKV attenuated strain 181/clone25. Subclinical CHIKV infections were identified by ≥8-fold rise from a baseline enrollment PRNT titer <10 without symptomatic infection detected during the intervening surveillance period. Selected CHIKV PCR-positive samples underwent viral isolation and envelope protein-1 gene sequencing. Of 853 subjects who completed all study procedures at 12 months, 19 symptomatic infections (2.19 per 100 person-years) and 87 subclinical infections (10.03 per 100 person-years) occurred. The ratio of subclinical-to-symptomatic infections was 4.6:1 varying with age from 2:1 in 6 month-5 year olds to 12:1 in those >50 years old. Baseline CHIKV PRNT titer ≥10 was associated with 100% (95%CI: 46.1, 100.0) protection from symptomatic CHIKV infection. Phylogenetic analysis demonstrated Asian genotype closely related to strains from Asia and the Caribbean.Conclusions
Subclinical infections accounted for a majority of total CHIKV infections. A positive baseline CHIKV PRNT titer was associated with protection from symptomatic CHIKV infection. These findings have implications for assessing disease burden, understanding virus transmission, and supporting vaccine development. 相似文献80.
Christi M. Terry Ilya Zhuplatov Yuxia He Tze-Chein Wun Seong-Eun Kim Alfred K. Cheung 《PloS one》2015,10(9)