全文获取类型
收费全文 | 886篇 |
免费 | 107篇 |
国内免费 | 4篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 13篇 |
2021年 | 35篇 |
2020年 | 16篇 |
2019年 | 22篇 |
2018年 | 22篇 |
2017年 | 17篇 |
2016年 | 35篇 |
2015年 | 53篇 |
2014年 | 47篇 |
2013年 | 62篇 |
2012年 | 73篇 |
2011年 | 82篇 |
2010年 | 44篇 |
2009年 | 43篇 |
2008年 | 60篇 |
2007年 | 75篇 |
2006年 | 54篇 |
2005年 | 50篇 |
2004年 | 57篇 |
2003年 | 28篇 |
2002年 | 33篇 |
2001年 | 5篇 |
2000年 | 1篇 |
1999年 | 6篇 |
1998年 | 13篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1994年 | 6篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 5篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1969年 | 2篇 |
排序方式: 共有997条查询结果,搜索用时 15 毫秒
991.
992.
Cranberry flavonoids prevent toxic rat liver mitochondrial damage in vivo and scavenge free radicals in vitro
下载免费PDF全文
![点击此处可从《Cell biochemistry and function》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Elena A. Lapshina Maria Zamaraeva Vitali T. Cheshchevik Ewa Olchowik‐Grabarek Szymon Sekowski Izabela Zukowska Nina G. Golovach Vasili N. Burd Ilya B. Zavodnik 《Cell biochemistry and function》2015,33(4):202-210
The present study was undertaken for further elucidation of the mechanisms of flavonoid biological activity, focusing on the antioxidative and protective effects of cranberry flavonoids in free radical‐generating systems and those on mitochondrial ultrastructure during carbon tetrachloride‐induced rat intoxication. Treatment of rats with cranberry flavonoids (7 mg/kg) during chronic carbon tetrachloride‐induced intoxication led to prevention of mitochondrial damage, including fragmentation, rupture and local loss of the outer mitochondrial membrane. In radical‐generating systems, cranberry flavonoids effectively scavenged nitric oxide (IC50 = 4.4 ± 0.4 µg/ml), superoxide anion radicals (IC50 = 2.8 ± 0.3 µg/ml) and hydroxyl radicals (IC50 = 53 ± 4 µg/ml). The IC50 for reduction of 1,1‐diphenyl‐2‐picrylhydrazyl radicals (DPPH) was 2.2 ± 0.3 µg/ml. Flavonoids prevented to some extent lipid peroxidation in liposomal membranes and glutathione oxidation in erythrocytes treated with UV irradiation or organic hydroperoxides as well as decreased the rigidity of the outer leaflet of the liposomal membranes. The hepatoprotective potential of cranberry flavonoids could be due to specific prevention of rat liver mitochondrial damage. The mitochondria‐addressed effects of flavonoids might be related both to radical‐scavenging properties and modulation of various mitochondrial events. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
993.
994.
995.
André Gomes-dos-Santos Elsa Froufe John M. Pfeiffer Nathan A. Johnson Chase H. Smith André M. Machado L. Filipe C. Castro Van Tu Do Akimasa Hattori Nicole Garrison Nathan V. Whelan Ivan N. Bolotov Ilya V. Vikhrev Alexander V. Kondakov Mohamed Ghamizi Vincent Prié Arthur E. Bogan Manuel Lopes Lima 《Molecular ecology resources》2023,23(6):1403-1422
The proliferation of genomic sequencing approaches has significantly impacted the field of phylogenetics. Target capture approaches provide a cost-effective, fast and easily applied strategy for phylogenetic inference of non-model organisms. However, several existing target capture processing pipelines are incapable of incorporating whole genome sequencing (WGS). Here, we develop a new pipeline for capture and de novo assembly of the targeted regions using whole genome re-sequencing reads. This new pipeline captured targeted loci accurately, and given its unbiased nature, can be used with any target capture probe set. Moreover, due to its low computational demand, this new pipeline may be ideal for users with limited resources and when high-coverage sequencing outputs are required. We demonstrate the utility of our approach by incorporating WGS data into the first comprehensive phylogenomic reconstruction of the freshwater mussel family Margaritiferidae. We also provide a catalogue of well-curated functional annotations of these previously uncharacterized freshwater mussel-specific target regions, representing a complementary tool for scrutinizing phylogenetic inferences while expanding future applications of the probe set. 相似文献
996.
997.
Artem S. Chebotarev Matvei S. Pochechuev Aleksandr A. Lanin Ilya V. Kelmanson Daria A. Kotova Elena S. Fetisova Anastasiya S. Panova Dmitry S. Bilan Andrei B. Fedotov Vsevolod V. Belousov Aleksei M. Zheltikov 《Journal of biophotonics》2021,14(3):e202000301
We present experiments on cell cultures and brain slices that demonstrate two-photon optogenetic pH sensing and pH-resolved brain imaging using a laser driver whose spectrum is carefully tailored to provide the maximum contrast of a ratiometric two-photon fluorescence readout from a high-brightness genetically encoded yellow-fluorescent-protein-based sensor, SypHer3s. Two spectrally isolated components of this laser field are set to induce two-photon-excited fluorescence (2PEF) by driving SypHer3s through one of two excitation pathways—via either the protonated or deprotonated states of its chromophore. With the spectrum of the laser field accurately adjusted for a maximum contrast of these two 2PEF signals, the ratio of their intensities is shown to provide a remarkably broad dynamic range for pH measurements, enabling high-contrast optogenetic deep-brain pH sensing and pH-resolved 2PEF imaging within a vast class of biological systems, ranging from cell cultures to the living brain. 相似文献