全文获取类型
收费全文 | 407篇 |
免费 | 25篇 |
出版年
2023年 | 4篇 |
2022年 | 1篇 |
2021年 | 10篇 |
2020年 | 7篇 |
2019年 | 3篇 |
2018年 | 11篇 |
2017年 | 9篇 |
2016年 | 13篇 |
2015年 | 15篇 |
2014年 | 26篇 |
2013年 | 27篇 |
2012年 | 35篇 |
2011年 | 38篇 |
2010年 | 25篇 |
2009年 | 12篇 |
2008年 | 41篇 |
2007年 | 27篇 |
2006年 | 24篇 |
2005年 | 22篇 |
2004年 | 13篇 |
2003年 | 22篇 |
2002年 | 20篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1987年 | 2篇 |
排序方式: 共有432条查询结果,搜索用时 15 毫秒
371.
Ilka Schnfelder Jrg Gelbrecht Jrg Schnfelder Christian E. W. Steinberg 《Journal of phycology》2002,38(1):66-89
We explored statistical relationships between the composition of littoral diatom assemblages and 21 chemical and physical environmental variables in 69 lakes and 15 river sites in the lowland of northeastern Germany. Canonical correspondence analysis with single treatment and with forward selection of environmental variables was used to detect 11 important ecological variables (dissolved inorganic carbon [DIC], Na + , total phosphorus [TP], dissolved organic carbon [DOC], total nitrogen [TN], pH, oxygen saturation, dissolved iron, SO42 ? , NH4 + , soluble reactive silicium) and maximum water depth or Ca2 + or soluble reactive phosphorus that most independently explain major proportions of the total diatom variance among the habitats. Monte Carlo permutation tests showed that each contributed a significant additional proportion (P < 0.05) of the variance in species composition. Together, these 11 most important environmental variables explained 34% of the total variance in species composition among the sites and captured 73% of the explained variance from the full 21 parameters model. Weighted‐averaging regression and calibration of 304 indicator taxa with tolerance down‐weighting and classic deshrinking was used to develop transfer functions between littoral diatoms and DIC, pH, TP, TN, and Cl ? . The DOC:TP ratio was introduced and a weighted‐averaging model was developed to infer allochthonous DOC effects in freshwater ecosystems. This diatom‐DOC/TP model was significant (P < 0.001) and explained 7.6% of the total diatom variance among the sites, surpassing the inferential power of the diatom‐TP‐transfer function (7.3% explained variance). The root‐mean‐square errors of prediction of the models were estimated by jack‐knifing and were comparable with published data sets from surface sediment diatom samples. The data set of littoral diatoms and environmental variables allows use of the diatom‐environmental transfer functions in biomonitoring and paleolimnological approaches across a broad array of natural water resources (such as floodplains, flushed lakes, estuaries, shallow lakes) in the central European lowland ecoregion. 相似文献
372.
Peter Escher Monika Eiblmeier Ilka Hetzger Heinz Rennenberg 《Physiologia plantarum》2003,117(1):72-78
In the present field study with adult trees inhabited by Viscum album , the question was addressed as to whether European mistletoes are able to remove reduced sulphur from the xylem sap of its hosts. For this purpose the reduced sulphur composition and content of the xylem sap of Viscum album and the corresponding hosts Populus × euramericana and Abies alba were analysed. The xylem sap of Viscum was enriched in reduced sulphur compared to the hosts but still reflected the higher reduced sulphur content of Populus compared to Abies . Despite similar xylem sap composition of the hosts with glutathione as the dominating thiol, Viscum on Populus contained predominantly cysteine, Viscum on Abies predominantly glutathione in its xylem sap. These findings suggest selective and different removal of reduced sulphur from these hosts. Still the amount of reduced sulphur removed was too small to result in changes of the concentration of thiols in the xylem sap of the hosts that are statistically significant, probably due to the high variability encountered under field conditions. Despite the differences in the reduced sulphur composition and contents of the xylem sap between Viscum on Populus and Viscum on Abies , total thiol content as well as thiol composition of Viscum leaves on the two hosts were similar throughout the seasons. The seasonal pattern in the thiol composition and contents of Viscum leaves showed high levels in spring and autumn and low levels in summer. The significance of these seasonal changes is discussed. 相似文献
373.
374.
Feller Ilka C. Berger Uta Chapman Samantha K. Dangremond Emily M. Dix Nicole G. Langley J. Adam Lovelock Catherine E. Osborne Todd Z. Shor Audrey C. Simpson Loraé T. 《Ecosystems》2023,26(4):800-814
Ecosystems - Low temperature stress is the primary factor determining the latitudinal limits of tropical plants. As the climate warms, tropical species are migrating poleward, displacing native... 相似文献
375.
Ilka Duldhardt Julia Gaebel Lukasz Chrzanowski Ivonne Nijenhuis Claus Härtig Frieder Schauer Hermann J. Heipieper 《Microbial biotechnology》2010,3(2):201-209
The effect of different solvents and pollutants on the cellular fatty acid composition of three bacterial strains: Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans, representatives of diverse predominant anaerobic metabolisms was investigated. As the prevailing adaptive mechanism in cells of T. aromatica and G. sulfurreducens whose cellular fatty acids patterns were dominated by palmitic acid (C16:0) and palmitoleic acid (C16:1cis), the cells reacted by an increase in the degree of saturation of their membrane fatty acids when grown in the presence of sublethal concentrations of the chemicals. Next to palmitic acid C16:0, the fatty acid pattern of D. multivorans was dominated by anteiso-branched fatty acids which are characteristic for several sulfate-reducing bacteria. The cells responded to the solvents with an increase in the ratio of straight-chain saturated (C14:0, C16:0, C18:0) to anteiso-branched fatty acids (C15:0anteiso, C17:0anteiso, C17:1anteisoΔ9cis). The results show that anaerobic bacteria react with similar mechanisms like aerobic bacteria in order to adapt their membrane to toxic organic solvents. The observed adaptive modifications on the level of membrane fatty acid composition can only be carried out with de novo synthesis of the fatty acids which is strictly related to cell growth. As the growth rates of anaerobic bacteria are generally much lower than in the so far investigated aerobic bacteria, this adaptive response needs more time in anaerobic bacteria. This might be one explanation for the previously observed higher sensitivity of anaerobic bacteria when compared with aerobic ones. 相似文献
376.
Winter M Sombroek D Dauth I Moehlenbrink J Scheuermann K Crone J Hofmann TG 《Nature cell biology》2008,10(7):812-824
The tumour suppressor HIPK2 is an important regulator of cell death induced by DNA damage, but how its activity is regulated remains largely unclear. Here we demonstrate that HIPK2 is an unstable protein that colocalizes and interacts with the E3 ubiquitin ligase Siah-1 in unstressed cells. Siah-1 knockdown increases HIPK2 stability and steady-state levels, whereas Siah-1 expression facilitates HIPK2 polyubiquitination, degradation and thereby inactivation. During recovery from sublethal DNA damage, HIPK2, which is stabilized on DNA damage, is degraded through a Siah-1-dependent, p53-controlled pathway. Downregulation of Siah-1 inhibits HIPK2 degradation and recovery from damage, driving the cells into apoptosis. We have also demonstrated that DNA damage triggers disruption of the HIPK2-Siah-1 complex, resulting in HIPK2 stabilization and activation. Disruption of the HIPK2-Siah-1 complex is mediated by the ATM/ATR pathway and involves ATM/ATR-dependent phosphorylation of Siah-1 at Ser 19. Our results provide a molecular framework for HIPK2 regulation in unstressed and damaged cells. 相似文献
377.
378.
Francis RC Reyle-Hahn MS Höhne C Klein A Theruvath I Donaubauer B Busch T Boemke W 《Laboratory animals》2008,42(3):338-349
The noble gas xenon seems to have minimal cardiovascular side-effects and so may be an ideal anaesthetic agent when investigating cardiovascular physiology. In comparison with standard modern anaesthetics, we investigated the haemodynamic and hormonal effects of xenon in Beagle dogs. After a 30 min baseline period, anaesthesia was induced with propofol and maintained with either (1) 1.2% isoflurane/70% nitrous oxide (N(2)O), (2) 0.8% isoflurane/0.5 microg/kg/min remifentanil or (3) 63% xenon/0.5 microg/kg/min remifentanil (n = 6 per group). Haemodynamics were recorded and blood samples taken before and 60 min after induction. Mean arterial blood pressure (MAP) was higher in conscious dogs than during isoflurane/N(2)O (86 +/- 2 vs. 65 +/- 2 mmHg, mean +/- SEM) and isoflurane/remifentanil anaesthesia (95 +/- 2 vs. 67 +/- 3 mmHg), whereas MAP did not decrease significantly in response to xenon/remifentanil anaesthesia (96 +/- 4 vs. 85 +/- 6 mmHg). Bradycardia was present during isoflurane/remifentanil (54 +/- 2/min) and xenon/remifentanil (40 +/- 3/min), but not during isoflurane/N(2)O anaesthesia (98 +/- 3/min, P < 0.05). Xenon/remifentanil anaesthesia induced the highest reduction in cardiac output (CO) (-61%), and the highest increase in systemic vascular resistance (+120%) among all treatment groups (P < 0.05). A simultaneous increase in endogenous adrenaline and noradrenaline concentrations could only be observed in the xenon/remifentanil group, whereas angiotensin II and vasopressin concentrations increased in all groups. In conclusion, xenon/remifentanil anaesthesia maintains MAP but reduces heart rate and CO and is associated with a considerable stimulation of vasopressor hormones in Beagle dogs. Therefore, xenon/remifentanil exerts a new quality of adverse haemodynamic effects different from volatile anaesthetics and may not perform better during studies of cardiovascular physiology. 相似文献
379.
An ADP/ATP-specific mitochondrial carrier protein in the microsporidian Antonospora locustae 总被引:1,自引:0,他引:1
The mitochondrion is one of the defining characteristics of eukaryotic cells, and to date, no eukaryotic lineage has been shown to have lost mitochondria entirely. In certain anaerobic or microaerophilic lineages, however, the mitochondrion has become severely reduced that it lacks a genome and no longer synthesizes ATP. One example of such a reduced organelle, called the mitosome, is found in microsporidian parasites. Only a handful of potential mitosomal proteins were found to be encoded in the complete genome of the microsporidian Encephalitozoon cuniculi, and significantly no proteins of the mitochondrial carrier family were identified. These carriers facilitate the transport of solutes across the inner mitochondrial membrane, are a means of communication between the mitochondrion and cytosol, and are abundant in organisms with aerobic mitochondria. Here, we report the characterization of a mitochondrial carrier protein in the microsporidian Antonospora locustae and demonstrate that the protein is heterologously targeted to mitochondria in Saccharomyces cerevisiae. The protein is phylogenetically allied to the NAD+ transporter of S. cerevisiae, but we show that it has high specificity for ATP and ADP when expressed in Escherichia coli. An ADP/ATP carrier may provide ATP for essential ATP-dependent mitosomal processes such as Hsp70-dependent protein import and export of iron-sulfur clusters to the cytosol. 相似文献
380.
Thieringer F Maass T Czochra P Klopcic B Conrad I Friebe D Schirmacher P Lohse AW Blessing M Galle PR Teufel A Kanzler S 《Gene》2008,423(1):23-28
Platelet derived growth factor (PDGF) plays a central role in repair mechanisms after acute and chronic tissue damage. To further evaluate the role of PDGF-A in liver fibrogenesis in vivo, we generated transgenic mice with hepatocyte-specific overexpression of PDGF-A using the CRP-gene promoter. Transgenic but not wildtype mice showed expression of PDGF-A mRNA in the liver. Hepatic PDGF-A overexpression was accompanied by a significant increase in hepatic procollagen III mRNA expression as well as TGF-beta1 expression. Liver histology showed increased deposition of extracellular matrix in transgenic but not in wildtype mice. PDGF-A-transgenic mice showed positive sinusoidal staining for alpha-SMA indicating an activation of hepatic stellate cells. Since the profibrogenic effect of PDGF-A was accompanied by increased TGF-beta1 protein concentration in the liver of transgenic mice, it can be postulated that PDGF-A upregulates expression of TGF-beta1 which is a strong activator of hepatic stellate cells. Thus, these results point towards a fibrosis induction by PDGF-A via the TGF-beta1 signalling pathway. In conclusion, expression and functional analysis of PDGF-A in the liver of transgenic mice suggest a relevant profibrogenic role of PDGF-A via TGF-beta1 induction. Counteracting PDGF-A may therefore be one of the effects of tyrosine kinase inhibitors which showed protective effects in animal models of liver fibrosis. 相似文献