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31.
Moreira JS Almeida RG Tavares LS Santos MO Viccini LF Vasconcelos IM Oliveira JT Raposo NR Dias SC Franco OL 《The protein journal》2011,30(1):32-38
Heavy agricultural losses are closely related to attacks by insect-pests and phytopathogens such as bacteria and fungi. Among
them, the fungus Botrytis cinerea can cause gray mold in more than 200 different species of plants, and is considered a challenging problem for agribusiness.
Fungicides are commonly used to control this pathogen because they are fast-working and easy to apply. However, the continuous
use of fungicides may promote the selection of resistant fungi and can also cause profound contamination in ecosystems. Aiming
to find alternative strategies to solve these problems, several studies have focused on searching for plant proteins and peptides
with antifungal activities (AFPs). With this in mind, this report shows the isolation and characterization of two novels antifungal
proteins from flowers of rosemary pepper (Lippia sidoides Cham.) with 10 and 15 kDa. Isolation was performed by using an Octyl-Sepharose hydrophobic column. In vitro bioassays indicated
that isolated proteins were able to inhibit B. cinerea development, but were not effective against all bacteria tested. Moreover, N-termini sequences indicate that both proteins
showed sequence homology with NBS–LRR R proteins with a lower molecular mass, suggesting possible protein fragmentation. Data
reported here could help in the development of biotechnological products for crop protection against phytopathogenic fungi
in the near future. 相似文献
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Nemere I 《Steroids》2005,70(5-7):455-457
There are currently two main candidates for the membrane receptor for 1,25(OH)2D3: the 1,25D3-MARRS protein/ERp57; and the classical VDR. The 1,25D3-MARRS protein is essential for hormone-stimulated phosphate and calcium uptake in chick intestinal cells, whereas the VDR is not. The 1,25D3-MARRS protein also shows a high degree of correlation with growth periods in which bone is rapidly formed, whereas the VDR does not. However, in rat enterocytes, both the 1,25D3-MARRS protein and the VDR play a role in the rapid, steroid-mediated uptake of phosphate or calcium. Therefore, the theory that alternate binding sites on the VDR for various analogs account for all membrane-initiated phenomena, is incorrect. 相似文献
34.
Gailus-Durner V Fuchs H Becker L Bolle I Brielmeier M Calzada-Wack J Elvert R Ehrhardt N Dalke C Franz TJ Grundner-Culemann E Hammelbacher S Hölter SM Hölzlwimmer G Horsch M Javaheri A Kalaydjiev SV Klempt M Kling E Kunder S Lengger C Lisse T Mijalski T Naton B Pedersen V Prehn C Przemeck G Racz I Reinhard C Reitmeir P Schneider I Schrewe A Steinkamp R Zybill C Adamski J Beckers J Behrendt H Favor J Graw J Heldmaier G Höfler H Ivandic B Katus H Kirchhof P Klingenspor M Klopstock T Lengeling A 《Nature methods》2005,2(6):403-404
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The identity of the histidine specific transfer RNA (tRNAHis) is largely determined by a unique guanosine residue at position −1. In eukaryotes and archaea, the tRNAHis guanylyltransferase (Thg1) catalyzes 3′-5′ addition of G to the 5′-terminus of tRNAHis. Here, we show that Thg1 also occurs in bacteria. We demonstrate in vitro Thg1 activity for recombinant enzymes from the two bacteria Bacillus thuringiensis and Myxococcus xanthus and provide a closer investigation of several archaeal Thg1. The reaction mechanism of prokaryotic Thg1 differs from eukaryotic enzymes, as it does not require ATP. Complementation of a yeast thg1 knockout strain with bacterial Thg1 verified in vivo activity and suggests a relaxed recognition of the discriminator base in bacteria. 相似文献
38.
Heme biosynthesis in Methanosarcina barkeri via a pathway involving two methylation reactions
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The methanogenic archaeon Methanosarcina barkeri synthesizes protoheme via precorrin-2, which is formed from uroporphyrinogen III in two consecutive methylation reactions utilizing S-adenosyl-L-methionine. The existence of this pathway, previously exclusively found in the sulfate-reducing delta-proteobacterium Desulfovibrio vulgaris, was demonstrated for M. barkeri via the incorporation of two methyl groups from methionine into protoheme. 相似文献
39.
Visualizing cytoskeleton dynamics in mammalian cells using a humanized variant of monomeric red fluorescent protein 总被引:1,自引:0,他引:1
Fluorescent proteins are versatile tools for live cell imaging studies. In particular, recent progress was achieved in the development of monomeric red fluorescent proteins (mRFPs) that show improved properties in respect to maturation and intracellular fluorescence. mRFPmars, a red fluorescent protein designed especially for the use in Dictyostelium, proved to be a brilliant label for different cytoskeletal elements. Here we report on the synthesis of a humanized version of a monomeric RFP, mRFPruby, which differs in sequence from mRFPmars in four amino acids and has a codon usage that is optimized for the application in mammalian cells. In order to demonstrate the usefulness of this new mRFP variant, mRFPruby fused to beta-actin was expressed in different mouse cell lines and used to visualize actin cytoskeleton dynamics by live cell microscopy. 相似文献
40.
Fredy D.A. Silva Ilka M. Vasconcelos Marina D.P. Lobo Patrícia G. de Castro Vladimir G. Magalhães Cléverson D.T. de Freitas Célia R.R.S. Carlini Paulo M. Pinto Leila M. Beltramini José H.A. Filho Eduardo B. Barros Luciana M.R. Alencar Thalles B. Grangeiro José T.A. Oliveira 《Biochimica et Biophysica Acta (BBA)/General Subjects》2012