排序方式: 共有88条查询结果,搜索用时 390 毫秒
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Corrigan DK Schulze H Henihan G Ciani I Giraud G Terry JG Walton AJ Pethig R Ghazal P Crain J Campbell CJ Mount AR Bachmann TT 《Biosensors & bioelectronics》2012,34(1):178-184
Using electrochemical impedance spectroscopy (EIS) the sensitive and specific detection of the antibiotic resistance gene mecA has been demonstrated. The gene sequence was obtained from clinical Staphylococcus aureus isolates. Initially a mecA specific probe was selected from hybridisation tests with a 3' and 5' version of a previously published probe sequence. When immobilised on a gold electrode in PNA form it was possible to detect hybridisation of mecA PCR product electrochemically at concentrations as low as 10nM. By incorporating an undecane-thiol and 1.8 nm glycol spacer into the PNA probe it was possible to extend the limit of detection for mecA to 10 pM. Most published studies on EIS and nucleic acid detection report the use of short artificial DNA sequences or novel signal amplification schemes which improve sensitivity whereas this study reports the successful detection of long DNA fragments produced by PCR following extraction from clinical isolates. Finally, using screen printed electrodes the paper demonstrates hybridisation monitoring of mecA in an "on-line" assay format under ambient conditions which paves the way for rapid mecA detection in point of care scenarios. 相似文献
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Martin Sikora Meredith L. Carpenter Andres Moreno-Estrada Brenna M. Henn Peter A. Underhill Federico Sánchez-Quinto Ilenia Zara Maristella Pitzalis Carlo Sidore Fabio Busonero Andrea Maschio Andrea Angius Chris Jones Javier Mendoza-Revilla Georgi Nekhrizov Diana Dimitrova Nikola Theodossiev Timothy T. Harkins Andreas Keller Frank Maixner Albert Zink Goncalo Abecasis Serena Sanna Francesco Cucca Carlos D. Bustamante 《PLoS genetics》2014,10(5)
Genome sequencing of the 5,300-year-old mummy of the Tyrolean Iceman, found in 1991 on a glacier near the border of Italy and Austria, has yielded new insights into his origin and relationship to modern European populations. A key finding of that study was an apparent recent common ancestry with individuals from Sardinia, based largely on the Y chromosome haplogroup and common autosomal SNP variation. Here, we compiled and analyzed genomic datasets from both modern and ancient Europeans, including genome sequence data from over 400 Sardinians and two ancient Thracians from Bulgaria, to investigate this result in greater detail and determine its implications for the genetic structure of Neolithic Europe. Using whole-genome sequencing data, we confirm that the Iceman is, indeed, most closely related to Sardinians. Furthermore, we show that this relationship extends to other individuals from cultural contexts associated with the spread of agriculture during the Neolithic transition, in contrast to individuals from a hunter-gatherer context. We hypothesize that this genetic affinity of ancient samples from different parts of Europe with Sardinians represents a common genetic component that was geographically widespread across Europe during the Neolithic, likely related to migrations and population expansions associated with the spread of agriculture. 相似文献
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Interfacial Engineering of P3HT/ZnO Hybrid Solar Cells Using Phthalocyanines: A Joint Theoretical and Experimental Investigation 下载免费PDF全文
Giuseppe Mattioli Sadok Ben Dkhil Maria Ilenia Saba Giuliano Malloci Claudio Melis Paola Alippi Francesco Filippone Paolo Giannozzi Anil Kumar Thakur Meriem Gaceur Olivier Margeat Abdou Karim Diallo Christine Videlot‐Ackermann Jörg Ackermann Aldo Amore Bonapasta Alessandro Mattoni 《Liver Transplantation》2014,4(12)
Atomistic simulations and experimental investigations are combined to study heterojunction interfaces of hybrid polymer solar cells, with the aim to better understand and precisely predict their photovoltaic properties. The focus is on a hybrid ternary model system based on a poly(3‐hexylthiophene) (P3HT)/zinc phthalocyanine (ZnPc)/ZnO interface, in which a ZnPc interlayer is applied to improve the performance of the hybrid interface. Theoretical predictions of the ternary system are validated against the properties of a concrete P3HT/ZnPc/ZnO planar heterojunction device. The theoretical predictions closely agree with the photovoltaic properties obtained in P3HT/ZnPc/ZnO solar cells, indicating the strength of the method for modeling hybrid heterojunction interfaces. The theoretical and experimental results reveal that: i) ZnPc molecules in direct contact with a ZnO surface insert new energy levels due to a strong ZnPc/ZnO coupling, ii) electron injection from these new energy levels of ZnPc into ZnO is highly efficient, iii) the ZnPc/ZnO coupling strongly influences the energy levels of the ZnO and P3HT leading to a reduction of the open circuit voltage, and iv) charge carrier recombination at the P3HT/ZnO interface is reduced by the ZnPc interlayer. The intercalation of ZnPc leads to an increase in photocurrent as well as to an overall increase in power conversion. 相似文献
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Silvina Ippi Wouter F. D. van Dongen Ilenia Lazzoni Cristóbal I. Venegas Rodrigo A. Vásquez 《Ethology : formerly Zeitschrift fur Tierpsychologie》2013,119(12):1107-1117
The high impact of predators on breeding success has favored the evolution of diverse behavioral strategies that enhance both individual survivorship and reproductive success. Self‐ and nest‐defense against predators are inter‐related behaviors that vary according to life history traits and the risks involved in defense. In addition, interpopulation differences in the composition of predator guilds, and hence predation pressures, may result in geographic variation in antipredator defense strategies. It is generally accepted that the absence of predators could drive the loss of antipredator behavior. However, it is unclear how species respond if one type of predator (e.g., a specialized nest predator) is absent but others are present. The multipredator hypothesis suggests that the absence of only a few predators is not sufficient to drive a change or loss of antipredator behavior in a species. We examined interpopulation variation in defense behavior in the thorn‐tailed rayadito (Aphrastura spinicauda), a suboscine bird inhabiting a wide latitudinal range in southern South America. In populations where nest predators were either present or absent, we examined the responses of rayaditos to an adult predator (an owl) and to a nest predator (a marsupial). Owl‐defense behavior involved more intense and longer responses than marsupial‐defense. This suggests that adult rayaditos may allocate more energy to defend themselves than their young. However, because the presence of adult predators near the nest also prevents parents from feeding the nestlings, owl‐defense may simultaneously represent a form of nest‐defense. No differences in marsupial‐defense were found among the three sampled populations, despite the absence of native mammalian nest predators in one population. The lack of variation in nest‐defense behavior provides some support for the multipredator hypothesis, although the high interpopulation gene flow in this species could also contribute to this behavior's maintenance. 相似文献
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Sbriccoli P Bazzucchi I Di Mario A Marzattinocci G Felici F 《Journal of strength and conditioning research / National Strength & Conditioning Association》2007,21(3):738-744
The main purposes of this study were to describe the cardiorespiratory fitness and lower limbs maximal muscle power of a selected group of Olympic Italian male (M) and female (F) judokas. Eleven subjects (6 M, 5 F) underwent 3 different tests. The VO(2)max and ventilatory threshold (VT; V-slope method) were assessed during a graded maximal treadmill test. Lower limbs muscle peak power (PP) and mean power (MP) were determined during a 30-second Wingate test (WIN). Post-WIN blood lactate peak was also measured. Subjects were tested also during a 5-minute combat test (CT), during which blood lactate and heart rate (HR) were monitored. VO(2)max (mean +/- SD) was 47.3 +/- 10.9 and 52.9 +/- 4.4 ml x kg(-1) x min(-1) for M and F judokas, respectively. The VT corresponded to 80.8% (M) and 86.5% (F) of VO(2)max. Both PP and MP, measured during the WIN, were significantly higher (p < 0.05) in M than in F judokas (PP: 12.1 +/- 2.4 vs. 9.5 +/- 1.1 W x kg(-1); MP: 5.4 +/- 1.1 W x kg(-1); F: 4.3 +/- 0.5 W x kg(-1)). Post WIN blood lactate peak was 6.9 +/- 2.8 mmol x l(-1) and 6.1 +/- 1.8 mmol x l(-1) for M and F judokas, respectively (not significant). During the CT blood lactate peak was 9.9 +/- 3.0 mmol x l(-1) (M) and 9.2 +/- 2.0 mmol x l(-1) (F); these values being significantly higher than those obtained after the WIN (p < 0.05). In conclusion, Italian Olympic judokas showed high levels of muscle power but accompanied by a moderate engagement of the aerobic metabolic pathway, which is well in accordance with the characteristics of judo. Having these results in top-level athletes may represent a useful contribution to the work of coaches and trainers in optimizing training programs for the achievement of the best performance of the judoka. 相似文献
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Anna Aspesi Elisa Pavesi Elisa Robotti Rossella Crescitelli Ilenia Boria Federica Avondo Hélène Moniz Lydie Da Costa Narla Mohandas Paola Roncaglia Ugo Ramenghi Antonella Ronchi Stefano Gustincich Simone Merlin Emilio Marengo Steven R. Ellis Antonia Follenzi Claudio Santoro Irma Dianzani 《Gene》2014
Defects in genes encoding ribosomal proteins cause Diamond Blackfan Anemia (DBA), a red cell aplasia often associated with physical abnormalities. Other bone marrow failure syndromes have been attributed to defects in ribosomal components but the link between erythropoiesis and the ribosome remains to be fully defined. Several lines of evidence suggest that defects in ribosome synthesis lead to “ribosomal stress” with p53 activation and either cell cycle arrest or induction of apoptosis. Pathways independent of p53 have also been proposed to play a role in DBA pathogenesis. 相似文献
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Paola Cipriani Paola Di Benedetto Piero Ruscitti Antonio Francesco Campese Vasiliki Liakouli Francesco Carubbi Ilenia Pantano Onorina Berardicurt Isabella Screpanti Roberto Giacomelli 《Arthritis research & therapy》2014,16(5)