全文获取类型
收费全文 | 1112篇 |
免费 | 80篇 |
出版年
2023年 | 6篇 |
2022年 | 16篇 |
2021年 | 27篇 |
2020年 | 20篇 |
2019年 | 27篇 |
2018年 | 45篇 |
2017年 | 34篇 |
2016年 | 57篇 |
2015年 | 72篇 |
2014年 | 89篇 |
2013年 | 81篇 |
2012年 | 121篇 |
2011年 | 89篇 |
2010年 | 64篇 |
2009年 | 49篇 |
2008年 | 79篇 |
2007年 | 52篇 |
2006年 | 49篇 |
2005年 | 44篇 |
2004年 | 53篇 |
2003年 | 45篇 |
2002年 | 31篇 |
2001年 | 6篇 |
2000年 | 7篇 |
1999年 | 9篇 |
1998年 | 6篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有1192条查询结果,搜索用时 812 毫秒
71.
Hagai Tavori Yan Ru Su Patricia G. Yancey Ilaria Giunzioni Ashley J. Wilhelm John L. Blakemore Manal Zabalawi MacRae F. Linton Mary G. Sorci-Thomas Sergio Fazio 《Journal of lipid research》2015,56(3):635-643
Tissue cholesterol accumulation, macrophage infiltration, and inflammation are features of atherosclerosis and some forms of dermatitis. HDL and its main protein, apoAI, are acceptors of excess cholesterol from macrophages; this process inhibits tissue inflammation. Recent epidemiologic and clinical trial evidence questions the role of HDL and its manipulation in cardiovascular disease. We investigated the effect of ectopic macrophage apoAI expression on atherosclerosis and dermatitis induced by the combination of hypercholesterolemia and absence of HDL in mice. Hematopoietic progenitor cells were transduced to express human apoAI and transplanted into lethally irradiated LDL receptor−/−/apoAI−/− mice, which were then placed on a high-fat diet for 16 weeks. Macrophage apoAI expression reduced aortic CD4+ T-cell levels (−39.8%), lesion size (−25%), and necrotic core area (−31.6%), without affecting serum HDL or aortic macrophage levels. Macrophage apoAI reduced skin cholesterol by 39.8%, restored skin morphology, and reduced skin CD4+ T-cell levels. Macrophage apoAI also reduced CD4+ T-cell levels (−32.9%) in skin-draining lymph nodes but had no effect on other T cells, B cells, dendritic cells, or macrophages compared with control transplanted mice. Thus, macrophage apoAI expression protects against atherosclerosis and dermatitis by reducing cholesterol accumulation and regulating CD4+ T-cell levels, without affecting serum HDL or tissue macrophage levels. 相似文献
72.
73.
Streptococcus agalactiae Capsule Polymer Length and Attachment Is Determined by the Proteins CpsABCD
74.
75.
Methyl jasmonate affects phenolic metabolism and gene expression in blueberry (Vaccinium corymbosum) 下载免费PDF全文
Giacomo Cocetta Mara Rossoni Claudio Gardana Ilaria Mignani Antonio Ferrante Anna Spinardi 《Physiologia plantarum》2015,153(2):269-283
Blueberry (Vaccinium corymbosum) is a fruit very much appreciated by consumers for its antioxidant potential and health‐promoting traits. Its beneficial potential properties are mainly due to a high content of anthocyanins and their amount can change after elicitation with methyl jasmonate. The aim of this work is to evaluate the changes in expression of several genes, accumulation of phenolic compounds and alterations in antioxidant potential in two different blueberry cultivars (‘Duke’ and ‘Blueray’) in response to methyl jasmonate (0.1 mM). Results showed that 9 h after treatment, the expression of phenylalanine ammonium lyase, chalcone synthase and anthocyanidin synthase genes was stimulated more in the ‘Blueray’ variety. Among the phenols measured an increase was recorded also for epicatechin and anthocyanin concentrations. ‘Duke’ is a richer sourche of anthocyanins compared to ‘Blueray’, treatment with methyl jasmonate promoted in ‘Blueray’ an increase in pigments as well as in the antioxidant potential, especially in fully ripe berries, but treated ‘Duke’ berries had greater levels, which were not induced by methyl jasmonate treatment. In conclusion, methyl jasmonate was, in some cases, an effective elicitor of phenolic metabolism and gene expression in blueberry, though with different intensity between cultivars. 相似文献
76.
Finore Ilaria Kasavi Ceyda Poli Annarita Romano Ida Oner Ebru Toksoy Kirdar Betul Dipasquale Laura Nicolaus Barbara Lama Licia 《World journal of microbiology & biotechnology》2011,27(10):2425-2433
This study reports the purification and biochemical characterization of a raw starch-digesting α-amylase from Geobacillus thermoleovorans subsp. stromboliensis subsp. nov. (strain PizzoT). The molecular weight was estimated to be 58 kDa by SDS–PAGE. The enzyme was highly active over a wide range of pH from
4.0–10.0. The optimum temperature of the enzyme was 70°C. It showed extreme thermostability in the presence of Ca2+, retaining 50% of its initial activity after 90 h at 70°C. The enzyme efficiently hydrolyzed 20% (w/v) of raw starches, concentration
normally used in starch industries. The α-amylase showed an high stability in presence of many organic solvents. In particular
the residual activity was of 73% in presence of 15% (v/v) ethyl alcohol, which corresponds to ethanol yield in yeast fermentation
process. By analyzing its complete amyA gene sequence (1,542 bp), the enzyme was proposed to be a new α-amylase. 相似文献
77.
Miles TJ Barfoot C Brooks G Brown P Chen D Dabbs S Davies DT Downie DL Eyrisch S Giordano I Gwynn MN Hennessy A Hoover J Huang J Jones G Markwell R Rittenhouse S Xiang H Pearson N 《Bioorganic & medicinal chemistry letters》2011,21(24):7483-7488
As part of our wider efforts to exploit novel mode of action antibacterials, we have discovered a series of cyclohexyl-amide compounds that has good Gram positive and Gram negative potency. The mechanism of action is via inhibition of bacterial topoisomerases II and IV. We have investigated various subunits in this series and report advanced studies on compound 7 which demonstrates good PK and in vivo efficacy properties. 相似文献
78.
Miles TJ Axten JM Barfoot C Brooks G Brown P Chen D Dabbs S Davies DT Downie DL Eyrisch S Gallagher T Giordano I Gwynn MN Hennessy A Hoover J Huang J Jones G Markwell R Miller WH Minthorn EA Rittenhouse S Seefeld M Pearson N 《Bioorganic & medicinal chemistry letters》2011,21(24):7489-7495
We have identified a series of amino-piperidine antibacterials with a good broad spectrum potency. We report the investigation of various subunits in this series and advanced studies on compound 8. Compound 8 possesses good pharmacokinetics, broad spectrum antibacterial activity and demonstrates oral efficacy in a rat lung infection model. 相似文献
79.
Microautophagy of cytosolic proteins by late endosomes 总被引:2,自引:0,他引:2
Sahu R Kaushik S Clement CC Cannizzo ES Scharf B Follenzi A Potolicchio I Nieves E Cuervo AM Santambrogio L 《Developmental cell》2011,20(1):131-139
Highlights? Late endosomes take up cytosolic proteins through membrane invaginations ? Endosomal microautophagy (eMI) requires multivesicular body formation ? hsc70 mediates selective targeting of cytosolic proteins during eMI ? hsc70 binds to the endosomal membrane through its polybasic cluster 相似文献
80.
Kongkamnerd J Milani A Cattoli G Terregino C Capua I Beneduce L Gallotta A Pengo P Fassina G Monthakantirat O Umehara K De-Eknamkul W Miertus S 《Journal of biomolecular screening》2011,16(7):755-764
Many assays aimed to test the inhibitory effects of synthetic molecules, and naturally occurring products on the neuraminidase activity exploit the hydrolysis of 2'-O-(4-methylumbelliferyl)-N-acetylneuraminic acid (4-MUNANA). The amount of the released product, 4-methylumbelliferone (4-MU), is then measured fluorimetrically. The authors attempted an analysis of the inhibitory properties of 35 naturally occurring flavonoids on neuraminidase N3, where only 29 of them were sufficiently soluble in the assay medium. During the analysis, the authors noticed a strong quenching effect due to the test compounds on the fluorescence of 4-MU. The quenching constants for the flavonoids were determined according to the Stern-Volmer approach. The extent of fluorescence reduction due to quenching and the magnitude of the fluorescence reduction measured in the inhibition assays were comparable: for 11 of 29 compounds, the two values were found to be coincident within the experimental uncertainty. These data were statistically analyzed for correlation by calculating the pertinent Pearson correlation coefficient. Inhibition and quenching were found to be positively correlated (r = 0.71, p(uncorr) = 1.5 × 10(-5)), and the correlation was maintained for the whole set of tested compounds. Altogether, the collected data imply that all of the tested flavonoids could produce false-positive results in the neuraminidase inhibition assay using 4-MUNANA as a substrate. 相似文献