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331.
Changes in neuronal activity modify the structure of dendritic spines and alter the function and protein composition of synapses. Regulated degradation of postsynaptic density (PSD) proteins by the ubiquitin-proteasome system is believed to play an important role in activity-dependent synaptic remodeling. Stimulating neuronal activity in vitro and in vivo induces the ubiquitination and degradation of GKAP/SAPAP and Shank, major scaffold proteins of the PSD. However, the specific ubiquitin ligases that regulate postsynaptic protein composition have not been identified. Here we identify the RING finger-containing protein TRIM3 as a specific E3 ubiquitin ligase for the PSD scaffold GKAP/SAPAP1. Present in PSD fractions from rat brain, TRIM3 stimulates ubiquitination and proteasome-dependent degradation of GKAP, and induces the loss of GKAP and associated scaffold Shank1 from postsynaptic sites. Suppression of endogenous TRIM3 by RNA interference (RNAi) results in increased accumulation of GKAP and Shank1 at synapses, as well as enlargement of dendritic spine heads. RNAi of TRIM3 also prevented the loss of GKAP induced by synaptic activity. Thus, TRIM3 is a novel E3 ligase that mediates activity-dependent turnover of PSD scaffold proteins and is a negative regulator of dendritic spine morphology. 相似文献
332.
The cytotoxic activity of human blood lymphocytes toward Raji cells was strongly elevated when human serum (HS) was included in the cytotoxicity assay. This phenomenon also occurred when the effector cells were activated by interferon (IFN). Hypogammaglobulinemic serum (HyS) and heat-inactivated serum could also augment cytotoxicity, but C3-depleted serum was inefficient. IFN treatment of Raji cells decreased their sensitivity to lysis and this effect was counteracted by addition of HS to the system. It is likely that C3 activation by, and deposition on, Raji cells when used as targets for cytotoxicity facilitate their recognition and lysis by lymphocytes. These events may represent one mechanism operating in the natural killing phenomenon. 相似文献
333.
1. The activity of leucyl-tRNA synthetase obtained from tobacco leaves declined by 50% over a period of 4 days senescence induced by detachment. In addition tRNA(Leu) from senescing leaves was charged to a lesser extent than tRNA(Leu) extracted from mature leaves immediately after detachment. 2. tRNA(Leu) was charged with a synthetase preparation from either mature or senescent leaves and chromatographed on an RPC 3 column. The elution profile showed that the marked decline in specific activity of leucyl-tRNA synthetase in senescent leaves was not associated with a loss of acylation of any isoacceptor of tRNA(Leu). 相似文献
334.
335.
Heike Bartels William S. Bennett Harly A. S. Hansen Miriam Eisenstein Shulamith Weinstein Jutta Müssig Niels Volkmann Frank Schlünzen Ilana Agmon Francois Franceschi Ada Yonath 《Biopolymers》1995,37(6):411-419
An electron density map of the large ribosomal subunit from Bacillus stearothermophilus was obtained at 26 Å resolution by single isomorphous replacement (SIR) from a derivative formed by specific quantitative labeling with a dense undecagold cluster. For derivalization, a mono-functional reagent of this cluster was bound to a sulfhydryl group of a purified ribosomal protein. which was in turn reconstituted with core particles of a mutant lacking this protein. The native, mutated, and derivatzed 50S ribosomal subunits crystallize under the same conditions in the same space group. Under favorable conditions, crystals of the derivatized subunit proved to be isomorphous with the native ones, whereas the crystals of the mutant may have somewhat different packing. After resolving the SIR phase ambiguity by solvent flattening, the electron density shows a packing that is consistent with the noncrystallographic symmetry found by Patterson searches as well as with the motif observed in electron micrographs of thin sections of the crystals. These studies established that phase information can be obtained from heavy metal clusters, even when the crystals under investigation are unstable and weakly diffracting. These results encouraged further effort at the construction of specifically derivatized crystals from other ribosomal particles that diffract to higher resolution. © 1994 John Wiley & Sons, Inc. 相似文献
336.
The expression of intermediate filaments is developmentally regulated. In the mammalian embryo keratins are the first to appear, followed by vimentin, while the principal intermediate filament of the adult brain is glial fibrillary acidic protein. The intermediate filaments expressed by a cell thus reflect its state of differentiation. The differentiation state of cells, and especially of glial cells, in turn determines their ability to support axonal growth. In this study we used three new antibodies directed against three fish intermediate filaments (glial fibrillary acidic protein, keratin 8 and vimentin), in order to determine the identity and level of expression of intermediate filaments present in fish glial cells in culture. We found that fish astrocytes and oligodendrocytes are both able to express keratin 8 and vimentin. We further demonstrate that under proliferative conditions astrocytes express high keratin 8 levels and most oligodendrocytes also express keratin 8, whereas under nonproliferative conditions the astrocytes express only low keratin 8 levels and most oligodendrocytes do not express keratin 8 at all. These results suggest that the fish glial cells retain characteristics of immature cells. The findings are also discussed in relation to the fish glial lineage. 相似文献
337.
Expression and reconstitution of biologically active human acetylcholinesterase fromEscherichia coli
Meir Fischer Avner Ittah Ilana Liefer Marian Gorecki 《Cellular and molecular neurobiology》1993,13(1):25-38
1. | Authentic human acetylcholinesterase (AChE) was expressed inEscherichia coli under regulation of the constitutivedeo promoter or the thermoinduciblePL promoter. |
2. | To facilitate expression in the prokaryotic system, recombinant human AChE (rhAChE) cDNA was modified at the N terminus by oligonucleotide substitutions in order to replace some of the GC-rich regions by AT. These modifications did not alter the amino acid sequence but resulted in ample production of the protein. |
3. | rhAChE accumulated in the cells and reached a level of 10% of total bacterial proteins. A partially purified inactive recombinant protein was recovered from inclusion bodies. |
4. | Active rhAChE was obtained after solubilization, folding, and oxidation, although the recovery of the active enzyme was low. A 20- to 40-fold increase in enzymatically active rhAChE was achieved by replacing Cys580 by serine. |
5. | The recombinant enzyme analogue was indistinguishable from native AChE isolated from erythrocytes in terms of substrate specificity and inhibitor selectivity. |
338.
D. Prusky I. Kobiler Y. Fishman J. J. Sims S. L. Midland N. T. Keen 《Journal of Phytopathology》1991,132(4):319-327
A new antifungal compound was isolated from peel and flesh of unripe avocado fruits and identified as 1-acetoxy-2,4-dihydroxy-n-heptadeca-16-ene. The maximal concentration of the anti-fungal monoene in unripe fruits was about 800 μg. g?1 fr.wt. During ripening the monoene decreased to 40 μg. g?1 fr.wt. concomitantly with the appearance of disease symptoms. The concentration of the previously described antifungal diene, 1-acetoxy-2-hydroxy-4-oxo-heneicosa-12,15-diene (Prusky et al. 1982), in avocado peel was 1,600 μg. g?1 fr.wt. in unripe fruits, decreasing during ripening to 120 μg. g?1 fr.wt. At 750 μg. ml?1 the inhibition of germ tube elongation of germinated conidia by the antifungal monoene and the antifungal diene was 15 % and 44 %, respectively. A 1: 1 mixture of both antifungal compounds in concentrations ranging from 50 to 750 μg. ml?1, showed synergistic activity and increased the percent of inhibited germ tubes of germinated conidia up to 15 % over the sum of activities of the separate compounds. The results are discussed in relation to the hypothesis that the antifungal diene and the antifungal monoene are involved in the quiescence of the germinated appressoria of Colletotrichum gloeosporioides in unripe avocado fruits. 相似文献
339.
Hanhineva K Rogachev I Kokko H Mintz-Oron S Venger I Kärenlampi S Aharoni A 《Phytochemistry》2008,69(13):2463-2481
Formation of flower organs and the subsequent pollination process require a coordinated spatial and temporal regulation of particular metabolic pathways. In this study a comparison has been made between the metabolite composition of individual flower organs of strawberry (Fragaria × ananassa) including the petal, sepal, stamen, pistil and the receptacle that gives rise to the strawberry fruit. Non-targeted metabolomics analysis of the semi-polar secondary metabolites by the use of UPLC-qTOF-MS was utilized in order to localize metabolites belonging to various chemical classes (e.g. ellagitannins, proanthocyanidins, flavonols, terpenoids, and spermidine derivatives) to the different flower organs. The vast majority of the tentatively identified metabolites were ellagitannins that accumulated in all five parts of the flower. Several metabolite classes were detected predominantly in certain flower organs, as for example spermidine derivatives were present uniquely in the stamen and pistil, and the proanthocyanidins were almost exclusively detected in the receptacle and sepals. The latter organ was also rich in terpenoids (i.e. triterpenoid and sesquiterpenoid derivatives) whereas phenolic acids and flavonols were the predominant classes of compounds detected in the petals. Furthermore, we observed extensive variation in the accumulation of metabolites from the same class in a single organ, particularly in the case of ellagitannins, and the flavonols quercetin, kaempferol and isorhamnetin. These results allude to spatially-restricted production of secondary metabolite classes and specialized derivatives in flowers that take part in implementing the unique program of individual organs in the floral life cycle. 相似文献
340.
Leikin-Frenkel A Parini P Konikoff FM Benthin L Leikin-Gobbi D Goldiner I Einarsson C Gilat T 《Archives of biochemistry and biophysics》2008,471(1):63-71
Fatty acid bile acid conjugates (FABACs) prevent and dissolve cholesterol gallstones and prevent diet induced fatty liver, in mice. The present studies aimed to test their hypocholesterolemic effects in mice. Gallstone susceptible (C57L/J) mice, on high fat (HFD) or regular diet (RD), were treated with the conjugate of cholic acid with arachidic acid (FABAC; Aramchol). FABAC reduced the elevated plasma cholesterol levels induced by the HFD. In C57L/J mice, FABAC reduced plasma cholesterol by 50% (p < 0.001). In mice fed HFD, hepatic cholesterol synthesis was reduced, whereas CYP7A1 activity and expression were increased by FABAC. The ratio of fecal bile acids/neutral sterols was increased, as was the total fecal sterol excretion. In conclusion, FABACs markedly reduce elevated plasma cholesterol in mice by reducing the hepatic synthesis of cholesterol, in conjunction with an increase of its catabolism and excretion from the body. 相似文献