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排序方式: 共有662条查询结果,搜索用时 15 毫秒
641.
Shimada Takashi L. Yamaguchi Katsushi Shigenobu Shuji Takahashi Hiro Murase Masataka Fukuyoshi Shuichi Hara-Nishimura Ikuko 《Journal of plant research》2020,133(3):383-392
Journal of Plant Research - Sterols are important lipid constituents of cellular membranes in plants and other organisms. Sterol homeostasis is under strict regulation in plants because excess... 相似文献
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Kinoshita Tetsu; Nishimura Mikio; Hara-Nishimura Ikuko 《Plant & cell physiology》1995,36(8):1555-1562
Vacuolar processing enzymes (VPEs) are responsible for the maturationof seed proteins. Southern blot analysis showed that a familyof genes for VPEs in Arabidopsis thaliana was composed of threegenes, for 相似文献
645.
Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae 总被引:1,自引:0,他引:1
Nagako Hiraiwa Mikio Nishimura Ikuko Hara-Nishimura 《The Plant journal : for cell and molecular biology》1997,12(4):819-829
Vacuolar processing enzymes (VPEs) are cysteine proteinases responsible for maturation of various vacuolar proteins in plants. A larger precursor to VPE synthesized on rough endoplasmic reticulum is converted to an active enzyme in the vacuoles. In this study, a precursor to castor bean VPE was expressed in a pep4 strain of the yeast Saccharomyces cerevisiae to examine the mechanism of activation of VPE. Two VPE proteins of 59 and 46 kDa were detected in the vacuoles of the transformant. They were glycosylated in the yeast cells, although VPE is not glycosylated in plant cells in spite of the presence of two N-linked glycosylation sites. During the growth of the transformant, the level of the 59 kDa VPE increased slightly until a rapid decrease occurred after 9 h. By contrast, the 46 kDa VPE appeared simultaneously with the disappearance of the 59 kDa VPE. Vacuolar processing activity increased with the accumulation of the 46 kDa VPE, but not of the 59 kDa VPE. The specific activity of the 46 kDa VPE was at a similar level to that of VPE in plant cells. The 46 kDa VPE instead of proteinase A mediated the conversion of procarboxypeptidase Y to the mature form. This indicates that proteinase A responsible for maturation of yeast vacuolar proteins can be replaced functionally by plant VPE. These findings suggest that an inactive VPE precursor synthesized on the endoplasmic reticulum is transported to the vacuoles in the yeast cells and then processed to make an active VPE by self-catalytic proteolysis within the vacuoles. 相似文献
646.
Takuo Nishida Hiroaki Matsumae Ikuko Machida Takeji Shibatani 《Biocatalysis and Biotransformation》1995,12(3):205-214
As a result of screening of microorganisms, Mucor ambiguus IFO 6742 was found to reduce methyl 2-chloro-3-(4-methoxyphenyl)-3-oxopropionate (2) to give methyl (2S,3R)-2-chloro-3-hydroxy-3-(4-methoxyphenyl)propionate [(2S,3R)-3] in good yield with high enantioselectivity. The resulting (2S, 3R)-3 was converted into methyl (2S,3R)-3-(4-methoxyphenyl)glycidate [(2S,3R)-4] by treatment with sodium methoxide. On the other hand, its enantiomer, (2R,3S)-4 was obtained by the Mitsunobu esterification of (2S,3R)-3 and subsequent treatment with sodium methoxide. Also (2R,3S)-4 was obtained by the treatment of (2RS,3S)-3, which was obtained from 2 by Trichoderma viride OUT 4642, with sodium methoxide. 相似文献
647.
Ikuko Yazaki 《Development, growth & differentiation》1993,35(6):671-682
A new substance (ES-1) which localizes on the ectodermal and espophageal epithelia of sea urchin embryos was identified by a monoclonal antibody, McA ES-1. McA ES-1 recognized a 175 KDa protein of fertilized and 200 KDa in proteins of unfertilized egg-cortices. By indirect fluorescent antibody staining, ES-1 was found on the plasma membrane of fertilized eggs and in the cortical region of unfertilized eggs. ES-1 was not contained in the cortical granules and remained fixed in the cortex after centrifugation of unfertilized eggs for 30 min at 20,000 g. The polarized localization of ES-1 on the apical surface of ectodermal epithelial cells continued to the metamorphosis. It disappeared from mesenchyme cells and other migrating cells of the gastrula, while ES-1 was reexpressed in the presumptive esophagus to be connected with ectodermal epithelium. This may suggest a functional significance of ES-1 in establishment of cell polarity in the epithelium of larvae. In metamorphosing larvae and adults, the apical localization of ES-1 could no longer be found, and it was found in coelomocytes. From these findings, it is concluded that ES-1 was a novel surface substance of embryos and is probably phagocytosed at metamorphosis. 相似文献
648.
Oil bodies are localized in the seed cells and leaf cells of many land plants. They have a passive function as storage organelles for lipids. We recently reported that the leaf oil body has an active function as a subcellular factory that produces an antifungal oxylipin during fungal infection in Arabidopsis thaliana. Here, we propose a model for oil body-mediated plant defense. Remarkably, senescent leaves develop oil bodies and accumulate α-dioxygenase1 (α-DOX1) and caleosin3 (CLO3) on the oil-body membrane, which catalyze the conversion of α-linolenic acid to the phytoalexin 2-hydroxy-octadecatrienoic acid (2-HOT). The model proposes that senescent leaves actively produce antifungal oxylipins and phytoalexins, and abscised leaves contain a mixture of antifungal compounds. In natural settings, the abscised leaves with antifungal compounds accumulate in leaf litter and function to protect healthy tissues and young plants from fungal infection. Plants might have evolved this ecological function for dead leaves. 相似文献
649.
Takashi Kobayashi Ikuko Kakizaki Hiroyuki Nozaka Toshiya Nakamura 《Biochemistry and Biophysics Reports》2017
Because cartilage lacks nerves, blood vessels, and lymphatic vessels, it is thought to contain factors that inhibit the growth and development of those tissues. Chondroitin sulfate proteoglycans (CSPGs) are a major extracellular component in cartilage. CSPGs contribute to joint flexibility and regulate extracellular signaling via their attached glycosaminoglycan, chondroitin sulfate (CS). CS and CSPG inhibit axonal regeneration; however, their role in blood vessel formation is largely unknown. To clarify the function of CSPG in blood vessel formation, we tested salmon nasal cartilage proteoglycan (PG), a member of the aggrecan family of CSPG, for endothelial capillary-like tube formation. Treatment with salmon PG inhibited endothelial cell adhesion and in vitro tube formation. The anti-angiogenic activity was derived from CS in the salmon PG but not the core protein. Salmon PG also reduced matrix metalloproteinase expression and inhibited angiogenesis in the chick chorioallantoic membrane. All of these data support an anti-angiogenic role for CSPG in cartilage. 相似文献
650.
Makoto Shirakawa Haruko Ueda Tomoo Shimada Takayuki Kohchi Ikuko Hara-Nishimura 《The Plant cell》2014,26(11):4448-4461
Myrosin cells, which accumulate myrosinase to produce toxic compounds when they are ruptured by herbivores, form specifically along leaf veins in Arabidopsis thaliana. However, the mechanism underlying this pattern formation is unknown. Here, we show that myrosin cell development requires the endocytosis-mediated polar localization of the auxin-efflux carrier PIN1 in leaf primordia. Defects in the endocytic/vacuolar SNAREs (syp22 and syp22 vti11) enhanced myrosin cell development. The syp22 phenotype was rescued by expressing SYP22 under the control of the PIN1 promoter. Additionally, myrosin cell development was enhanced either by lacking the activator of endocytic/vacuolar RAB5 GTPase (VPS9A) or by PIN1 promoter-driven expression of a dominant-negative form of RAB5 GTPase (ARA7). By contrast, myrosin cell development was not affected by deficiencies of vacuolar trafficking factors, including the vacuolar sorting receptor VSR1 and the retromer components VPS29 and VPS35, suggesting that endocytic pathway rather than vacuolar trafficking pathway is important for myrosin cell development. The phosphomimic PIN1 variant (PIN1-Asp), which is unable to be polarized, caused myrosin cells to form not only along leaf vein but also in the intervein leaf area. We propose that Brassicales plants might arrange myrosin cells near vascular cells in order to protect the flux of nutrients and water via polar PIN1 localization. 相似文献