土曲霉金色变种AT8951菊粉酶的纯化和性质的研究

土典霉金色变种AT8951菊粉酶粗酶液经硫酸铵分段沉淀、DEAE Cellulose DE32离子交换、超滤、Sephadex G-150凝胶过滤和FPLC,获得两个菊粉酶组分EⅠ和EⅡ,经分析型FPLG和PAGE鉴定为单一纯和分析纯。EⅠ分子量为66KD,最适作用温度和pH分别55℃和5.8;EⅡ分子量为56KD,最适作用温度为57℃,最适pH为6.0。EⅠ和EⅡ皆为糖蛋白,多糖含量分别为24.7％和22％,都属于内切酶。本文还对EⅠ和EⅡ的Km值和I/s值,温度、pH、离子对酶活作用的影响等进行了研究。     

Apoptosis in breast cancer: Nigerian vs. Finnish material

OBJECTIVE: To evaluate apoptotic activity in breast cancer from Nigerian (n = 300) and Finnish (n=285) women. STUDY DESIGN: Apoptotic bodies were expressed as square millimeters of neoplastic tissue (apoptotic index [AI]). The standardized mitotic index (SMI) and mitotic activity index (MAI) estimated proliferation. RESULTS: The mean (+/- SD) AI was higher in Nigeria (9.6+/-14.8/mm2) than in Finland (5.2+/-6.1/ mm2). In both populations, AI values were higher in premenopausal than postmenopausal women, in lymph node positive than lymph node negative tumors and in larger than smaller tumors. However, the differences were not statistically significant. Increasing histologic grade was associated with increasing AI values (Nigeria, P =.012; Finland, P= .0001). AI in infiltrating ductal carcinomas were higher than in special types of breast cancer (Nigeria, P = .0700; Finland, P = .0168). As a continuous variable, AI was a significant prognosticator (Nigeria, P = .0125, Finland, P = .0466). Increasing AI appeared to be associated with tumor progression and dedifferentiation. The higher SMI/AI in Nigeria (9.2) than in Finland (4.5) reflects higher proliferative activity in the Nigerian material. In multivariate analysis of AI, SMI, MAI and tumor size, the proliferative indices (SMI and MAI) and tumor size only were significant independent prognosticators. CONCLUSION: In Nigerian and Finnish material, AI has limited prognostic value as a tool in grading breast cancer. The higher mean SMI/AI in Nigerian cancer suggests a shift in the proliferation/cell death balance, which may be associated with a later phase of the cancer progression cascade.     

昆虫病原细菌“010”的鉴定

1970年初,我们发现从蜜蜂幼虫中分离的芽孢杆菌“010”在生产性能和对昆虫的致病方面,均显示了良好的特性。该年我组曾与南苑公社化工厂共同对此菌的生产及毒效进行了初步摸索。继之,考察了它的营养要求及某些生产工艺条件,并对不同害虫进行了室内及大田的毒力测定。1971—1972年,通过培养基试验和上罐考察,选出两组综合利用较为理想的培养基。其中一组配比是:豆饼粉2.4％、玉米浆2％、麦芽粉4％、碳酸钙0.1％。菌数曾达48亿/毫升。“010”杀虫范     

Comparing global models of terrestrial net primary productivity (NPP): analysis of differences in light absorption and light-use efficiency

Twelve global net primary productivity (NPP) models were compared: BIOME3, CASA, CARAIB, FBM, GLO-PEM, HYBRID, KGBM, PLAI, SDBM, SIB2, SILVAN and TURC. These models all use solar radiation as an input, and compute either absorbed solar radiation directly, or the amount of leaves used to absorb solar radiation, represented by the leaf area index (LAI). For all models, we obtained or estimated photosynthetically active radiation absorbed by the canopy (APAR). We then computed the light use efficiency for NPP (LUE) on an annual basis as the ratio of NPP to APAR. We analysed the relative importance for NPP of APAR and LUE. The analyses consider the global values of these factors, their spatial patterns represented by latitudinal variations, and the overall grid cell by grid cell variability. Spatial variability in NPP within a model proved to be determined by APAR, and differences among models by LUE. There was a compensation between APAR and LUE, so that global NPP values fell within the range of ‘generally accepted values’. Overall, APAR was lower for satellite driven models than for the other models. Most computed values of LUE were within the range of published values, except for one model.     

我国几种土壤甲螨包括一新种及二新亚种记述（蜱螨亚纲：甲螨亚目）

本文报道了新近采得的我国土壤甲螨六种,其中新种和新亚种定名为:中华弯步甲螨Gibbicepheus chinensis sp. nov.,长新领甲螨帽尔山亚种Caenosamerus spatiosus maoershanensis subsp. nov.,克氏蕾甲螨帽尔山亚种Gemmazetes crosby maoershanensis subsp. nov.,并记述了我国新纪录甲螨三种:大全罗甲螨Perlohm(?) gigantea(Aoki,1960)、水沢,德之甲螨乳Tolamocecheus mizusouai Aoki,1966及沼沢梁甲螨Lamellobates palustris Hammer,1958。     

安徽宁国毛竹山发现的旧石器早期遗存

1997年在安徽宁国毛竹山发掘的旧石器时代早期遗存 ,轮廓略呈半圆形 ,长 10m、宽 6m ,由 110 0多件砾石和石制品构成 ,中间部分是面积 4 7× 4m2 的空白区。遗存埋藏在网纹红土的底部 ,地质时代为中更新世中期 ,距今约 6 0万年。此类现象在中国旧石器时代早期尚属首次发现 ,可能属于储料场和石器制造场 ,也可能还有其他用途。     

Comparing global models of terrestrial net primary productivity (NPP): analysis of the seasonal atmospheric CO2 signal

Eight terrestrial biospheric models (TBMs) calculating the monthly distributions of both net primary productivity (NPP) and soil heterotrophic respiration (R_H) in the Potsdam NPP Model Intercomparison workshop are used to simulate seasonal patterns of atmospheric CO₂ concentration. For each model, we used net ecosystem productivity (NEP = NPP – R_H) as the source function in the TM2 atmospheric transport model from the Max-Planck Institute for Meteorology. Comparing the simulated concentration fields with detrended measurements from 25 monitoring stations spread over the world, we found that the decreasing seasonal amplitude from north to south is rather well reproduced by all the models, though the amplitudes are slightly too low in the north. The agreement between the simulated and observed seasonality is good in the northern hemisphere, but poor in the southern hemisphere, even when the ocean is accounted for. Based on a Fourier analysis of the calculated zonal atmospheric signals, tropical NEP plays a key role in the seasonal cycle of the atmospheric CO₂ in the whole southern hemisphere. The relatively poor match between measured and predicted atmospheric CO₂ in this hemisphere suggests problems with all the models. The simulation of water relations, a dominant regulator of NEP in the tropics, is a leading candidate for the source of these problems.     

中国长管蚜亚科19种新纪录

我们在东北农林蚜虫区系考察中发现下述长管蚜亚科19种为我国首次纪录。 1.茜草叶无网蚜Acyrthosiphon rubifoliae(Shinji)此种名为一疑难名称(nomen dubium),我们采得此种与原记述特征相符。寄主:茜草*。分布:吉林(长白山);日本。 2.日本忍冬圆尾蚜Amphicercidus japonicus(Hori)寄主:莫罗氏忍冬、忍冬属植物*。分布:辽宁(沈阳);日本。 3.飞帘短尾蚜Brachycadus(Acaudus)cardui(L.)寄主:第一寄主李、榆叶梅*、樱桃李、乌荆子、杏、小樱桃、甜樱桃;第二寄主菊科、紫草科。