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91.
Methenamine-Silver Staining: a Simple and Sensitive Staining Method for Senile Plaques and Neurofibrillary Tangles 总被引:1,自引:0,他引:1
Chie Haga Kenji Ikeda Kiyoshi Iwabuchi Haruhiko Akiyama Hiromi Kondoh Kenji Kosaka 《Biotechnic & histochemistry》1994,69(5):295-300
An improved methenamine-silver impregnation method is presented which exhibits sensitivity for amyloid substances comparable to that of anti-β protein immunostaining. In optimally treated sections, this technique stained both β-amyloid deposits and neurofibrillary tangles, which are known to have a β-pleated structure. This simple procedure allows a large number of sections to be stained for routine examination. 相似文献
92.
93.
Tsuyoshi Kihara Masato Ikeda Hiroshi Miyazaki Akira Matsushita 《Journal of neurochemistry》1993,61(5):1859-1864
Abstract: The in vivo microdialysis method was used to study the effect of the cholecystokinin-related peptide, ceruletide, on extracellular levels of dopamine (DA) in the striatum following perfusion with various K+ concentrations. Increasing the K+ concentration in the perfusate from 4 to 15 or 17.5 m M did not change basal DA release or release evoked by electrical stimulation of the medial forebrain bundle (MFB). However, when the perfusing solution contained 20 or 30 m M K+ , dose-dependent reductions of both basal and MFB-stimulated DA release occurred. Subcutaneous administration of ceruletide at 160 μg/kg had no influence on the basal or MFB-stimulated DA release with 4 or 15 m M K+ in the perfusate. However, after perfusion with 17.5 m M K+ , ceruletide significantly attenuated the basal and MFB-stimulated DA release. Carbachol (10 μ M ) locally applied via the dialysis probe also attenuated MFB-stimulated DA release after perfusion with 17.5 m M K+ . From these results, we conclude that under appropriate depolarization of striatal DA terminals, ceruletide induces further depolarization and inactivation of nigrostriatal DA terminals. The present data suggest that this effect may be mediated via intrinsic cholinergic neurons in the striatum. 相似文献
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We consider the problem of comparing several nucleic acid sequencesto identify words occurring imperfectly (patterns with no gap)with unusual frequency. Methods for computing, representing,and inspecting interactively the structure of such repeatingmotifs in nucleic acids and more generally any text are described.Multiple sequences are treated as one large concatenate. Ina preprocessing step, a lexical index is created to providerapid string matching for the enumeration of the words matchinga pattern. For given word features (word length, minimal frequency),a sequence profile is displayed. The profile can be inspectedinteractively with on-line algorithms. Applications to the identificationof regulatory elements in DNA regions involved in the controlof gene expression are presented. Our program (DNA-Lexemics)runs on the Macintosh. 相似文献
96.
Dispersal propensity of green leafhoppers was compared between a tropical species, Nephotettix virescens, and a temperate species, N. cincticeps. The flight ability was measured with tethered flight technique under laboratory conditions (25°C, 16L-8D). The pre-flight period was shorter and the flight duration was longer in N. virescens than in N. cincticeps in both sexes. No significant correlations were found between the flight activity and morphometric characters for either of the two species. The results suggested that dispersal propensity of N. virescens is higher than that of N. cincticeps. 相似文献
97.
Sakaba Tomoka Soejima Akiko Fujii Shinji Ikeda Hajime Iwasaki Takaya Saito Hiroaki Suyama Yoshihisa Matsuo Ayumi Kozhevnikov Andrey E. Kozhevnikova Zoya V. Wang Hongfeng Wang Siqi Pak Jae-Hong Fujii Noriyuki 《Journal of plant research》2023,136(4):437-452
Journal of Plant Research - A group of temperate grassland plant species termed the “Mansen elements” occurs in Japan and is widely distributed in the grasslands of continental East... 相似文献
98.
Summary The hybrid produced between a Carbondale haploid strain (-methyl-glucoside rapid fermenter) and a haploid strain (non-fermenter), derived from a hybrid between a homothallic and a heterothallicSaccharomyces, showed an irregular segregation pattern with regard to the fermentation of this sugar.To explain this irregularity, three pairs of alleles,MG
1/mg
1,MG
2/mg
2 andMG
3/mg
3, were assumed to be in quantitative control of the fermetation. Haploid cultures carrying the genotypes (1)mg
1
mg
2
mg
3, (2)MG
1
mg
2
mg
3, (3)mg
1
MG
2
mg
3, (4)mg
1
mg
2
MG
3, (5)MG
1
MG
2
mg
3, (6)MG
1
mg
2
MG
3, (7)mg
1
MG
2
MG
3, and (8)MG
1
MG
2
MG
3, were actually recovered. Strains equipped with: either (1) or (2); either (4) or (6); (3); (5); (7); or (8) are non-fermenters, extremely-slow-fermenters, slow-fermenters, medium-fermenters, semi-rapid-fermenters and rapid-fermenters respectively.The role of these genes in sugar fermentation and the identity or nonidentity of some of these genes with maltose and sucrose genes was discussed.With 2 Figures in the Text 相似文献
99.
100.