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501.
Pretreatment effects of different gibberellins, helminthosporicacid, cyclic AMP and Kinetin on subsequent IAA-induced elongationwere tested in cucumber hypocotyl sections. Gibberellin A7 wasmore active than GA3, while gibberellin A3 was almost inactive.Both helminthosporic acid and cyclic AMP mimicked GA3-action,though the degree of their activity was less. Kinetin pretreatmentresulted in marked inhibition of IAA-induced elongation. Thepretreatment effect of GA3 was also reflected in a greater responceof the sections to synthetic auxins. (Received October 6, 1973; ) 相似文献
502.
503.
Hiroshi Imagawa Haruko Yamano Kazuhiko Inoue Yoshinori Takino 《Bioscience, biotechnology, and biochemistry》2013,77(11):2337-2342
Three adenosine nucleosidases (adenosine ribohydrolase, EC 3.2.2.7) with high substrate specificity were isolated from the extracts of tea leaves by a procedure including fractionation with ammonium sulfate, column chromatography on DEAE- and CM-cellulose, and gel filtration on Sephadex G-100. They were designated adenosine nucleosidase I, II and III, respectively, and their properties were characterized.Among the naturally occurring nucleosides only adenosine and 2′-deoxyadenosine were hydrolyzed by these three enzymes and cleavage rate of the N-glycosidic bond in 2′-deoxyadenosine was three or four times greater than that in adenosine. 相似文献
504.
X.-L. Zhang Y. Komada Yan-Wen Zhou Tong-Xin Chen Haruko Sakai Eiichi Azuma Masaru Ido Minoru Sakurai 《Cancer immunology, immunotherapy : CII》1997,44(1):41-47
Peripheral blood lymphocytes obtained from children with acute lymphoblastic leukemia (ALL) at onset were studied for the
expression of interleukin-2 (IL-2) receptor α-chain (CD25) by two-color flow-cytometric analysis. Stimulated with anti-CD3
monoclonal antibody (mAb) alone, CD25 expression was significantly suppressed in CD4+ T cells from 27 of 48 (56.3%) cases and in CD8+ T cells from 29 of 48 (60.4%) cases. When stimulated with anti-CD3 mAb plus phorbol 12-myristate 13-acetate (PMA), CD25 expression
was clearly restored in certain cases of ALL. When PMA plus ionomycin were used for stimulation of T cells, CD25 was inducible
in a majority of cases. Interestingly CD25 expression upon anti-CD3 mAb stimulation was recovered after complete remission
had been achieved. These observations suggest the presence in ALL children at onset of an in vitro defect in the signal transduction
pathway of the T-cell-receptor/CD3 complex, resulting in inefficient CD25 expression. However, immune-staining analysis indicated
that protein kinase C was normally translocated from the cytosol fraction to the cell membrane fraction. The mobilization
of cytoplasmic free calcium is also normal.
Received: 27 March 1996 / Accepted: 23 December 1996 相似文献