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61.
Aphids ingest from the sieve tubes and by doing so they are confronted with sieve-tube occlusion mechanisms, which are part of the plant defense system. Because aphids are able to feed over longer periods, they must be able to prevent occlusion of the sieve plates induced by stylet penetration. Occlusion probably depends upon Ca2+-influx into the sieve element (SE) lumen. Aphid behavior, biochemical tests and in vitro experiments demonstrated that aphid''s watery saliva, injected during initial phase of a stylet penetration into the SE lumen, contains proteins that are able to bind calcium and prevent calcium-induced SE occlusion. In this addendum, we speculate on the consequences of saliva secretion for plant resistance. (a) The release of elicitors (e.g., oligogalacturonides) due to cell wall digestion by gel saliva enzymes may increase the resistance of cortex, phloem parenchyma cells and companion cells (CC) around the puncture site. (b) Ca2+-binding by aphid watery saliva may suppress the local defense responses in the SEs. (c) Signaling cascades triggered in CCs may lead to systemic resistance.Key words: aphid saliva, calcium binding, elicitor, oligogalacturonides, local plant defense, systemic plant defense, phloem translocation, aphid/plant-interactionAfter having penetrated the sieve-element (SE) plasma membrane, aphids encounter unspecific wound-induced occlusion reactions to prevent sap leakage.1–4 Occlusion mechanisms by callose, structural P-proteins and forisomes are likely induced by a sudden calcium influx into the sieve-tube lumen.5 Calcium possibly enters the sieve-tube lumen through the stylet wounding-site in the plasma membrane and/or stretch-activated calcium-channels.6–8 After SE penetration, aphids secrete watery saliva that contains calcium-binding proteins presumed to sabotage sieve-plate occlusion.9,10We demonstrated that Megoura viciae (Buckton) is most likely able to prevent or reduce sieve-tube occlusion in Vicia faba by secretion of watery saliva. By in vitro confrontation of isolated forisomes, protein bodies responsible for sieve-tube occlusion in Fabaceaen,5 and watery saliva concentrate, we were able to show that salivary proteins convey forisomes from a dispersed (+Ca2+) into a condensed (−Ca2+) state.10 The dispersed forisome functions in vivo as a plug, leading to stoppage of mass flow.5This in vitro evidence was corroborated by aphid behavior in response to leaf tip burning, which triggers an electrical potential wave (EPW) along the sieve tubes. Such an EPW induces Ca2+-influx and corresponding SE occlusion along the pathway.11 The passage of the EPW is associated with a prolonged secretion of watery saliva of aphids. This is interpreted as an attempt to unplug the SEs by calcium binding.10 Similar behavioral changes in response to leaf-tip burning were observed in an extended set of aphid/plant species combinations, indicating that attempted sabotage of sieve-tube occlusion by aphid saliva is a widespread phenomenon (unpublished).Aphid feeding was reported to induce local (on the same leaf) and systemic (in distant leaves) reactions of the host plant. The local response led to enhanced feeding,12–14 while the systemic response showed reduced ingestion and extended periods of watery saliva secretion in sieve tubes distant from previous feeding sites.12–14 These contrasting observations were described to be independent of the aphid species.13 The question arises how aphids induce these seemingly opposite plant responses?The aphid stylet pushing forward through cortical and vascular tissue is surrounded by a sheath of gel saliva, secreted into the apoplast.15,16 Gel saliva contains cellulase and pectinase that amongst others produce oligogalacturonides (OGs) along the stylet sheath by digestion of cell wall material.17,18 Usually, OGs act as elicitors, triggering a variety of plant responses against pathogens and insects in which the activation of calcium channels is involved.19,20 This seems to conflict with a suppression of resistance as observed for the impact of watery saliva in SEs.10 We will make an attempt to explain this paradoxon.OG induced defense responses may be triggered in all cell types adjacent to the salivary sheath (Fig. 1). Because watery saliva is only secreted briefly into these cells, which are punctured for orientation purposes (Hewer et al., unpublished), it seems unlikely that OG induced defense is suppressed here by saliva-mediated calcium binding.15 The diffusion range of OGs may be restricted to the close vicinity of the stylet sheath leading to an enhanced regional defense with a limited sphere of action (Fig. 1). Because the settling distance of aphids is restricted by their body size (1–10 mm),21 aphids feeding on the same leaf are probably hardly confronted with the regional defense induced by another aphid (Fig. 1). Otherwise, they would show an increased number of test probes before first phloem activity, as described for volatile mediated plant defense in cortex cells.13 Circumstantial support in favor of our hypothesis is provided by production of hydrogen peroxide in the apoplast,22 which is most likely associated with the action of OGs.22 Observations of hydrogen peroxide production during aphid (Macrosiphum euphorbiae) infestation of tomato in a limited area along the leaf veins, the preferred feeding sites of this species, indicate a locally restricted defense response (Fig. 1 and and22).4 The question arises why the cell signals are not spread via plasmodesmata to adjacent cells to induce resistance in a more extended leaf area? Dissemination of the signals may be prevented by closure of plasmodesmata (Fig. 1) through callose deposition,23,24 which is most likely directly coupled with calcium influx induced by OGs,25 by apoplastic hydrogen peroxide and to a minor extent by stylet puncture (Fig. 2).7,26Open in a separate windowFigure 1Hypothetical model on how stylet penetration induces and suppresses plant defense. Sheath saliva (light blue) that envelopes the stylet during propagation through the apoplast contains cellulase and pectinase,17,18 enzymes producing elicitors (e.g., oligogalacturonides (oGs)) by local cell wall digestion.19 Parenchyma cells adjacent to the sheath may develop a defense response owing to signaling cascades triggered by oG-mediated Ca2+-influx.19 Together with a Ca2+-dependent transient closure of plasmodesmata by callose (black crosses),23,24 the focused production of oGs may cause a defense response with a limited sphere of action (red—strong, brown—light, green—none). This restricted domain of defense may not be perceived by other aphids, since the settling distance is limited by the aphid body size. Nearby aphids do not show any sign of defense perception in their probing and feeding behavior.14 Signaling cascade compounds may be channeled from parenchyma cells to CCs (dashed yellow arrows), where they are subsequently released into the SEs. There they may act as long-distance systemic defense components (grey arrows). In contrast to the parenchyma domain (where only minor amounts of watery saliva are secreted), Ca2+-mediated reactions such as defense cascades and sieve-plate (SP) occlusion are suppressed in SEs by large amounts of watery saliva. The left aphid penetrates an SE and injects watery saliva (red cloud; ws) that inhibits local sieve-plate occlusion and,10 most likely, is transported by mass flow (black arrow) to adjacent SEs,27 where occlusion is impeded as well. A short-distance systemic spread over a few centimeters may explain local suppression of plant defense resulting in a higher rate of colonization. Salivary proteins or their degradation products may serve as systemic defense signals as well (grey arrows), but may also diffuse via the PPUs into CCs where additional systemic signals are induced (yellow arrows).Open in a separate windowFigure 2Hypothetical involvement of Ca2+-channels in aphid-induced cell defense (detail). During probing with its stylet the aphid secretes gel saliva as a lubrication substance (light blue) into the apoplast.15 on the way to the sieve tubes, aphids briefly puncture most non-phloem cells (red) after which the puncturing sites are sealed with gel saliva.7,16 Gel saliva also most likely prevents the influx of apoplastic calcium into pierced sieve elements (green) by sealing the penetration site.7 Watery saliva (red cloud), injected into the SE lumen,9 contains proteins which bind calcium ions (marked by X) that enter the SE via e.g., mechano sensitive Ca2+-channels activated by stylet penetration (blue tons).10 In this way, aphids suppress SE occlusion and activation of local defense cascades. In the parenchyma cells around the gel saliva sheath, a small cylindrical zone of defense may be induced by oligogalacturonides (oGs; brown triangles) produced by cell wall (grey) digestion.17–19 Perceived by unknown receptor proteins (R; e.g., a receptor like protein kinase)34 and kinase mediation (black dotted and dashed arrows), oGs lead to a Ca2+-influx through kinase activated calcium channels (orange tons).25 Around the probing site, aphids apparently induce the production of superoxide by Ca2+-induced activation of the NADPH oxidase (violet box) and its following conversion to hydrogen peroxide (red spots) is mediated by superoxide dismutase (SoD).4 Hydrogen peroxide activates Ca2+-channels (violet tons) and diffuses through plasma membrane (curled arrows) therefore potentially acting as a intracellular signal.26By contrast, Ca2+-influx into SEs, induced by presence of OGs or stylet insertion (Fig. 2), is not expected to trigger local defense given the abundant excretion of Ca2+-binding watery saliva.7,10,25 Watery saliva may spread to down-stream and adjacent SEs through transverse and lateral sieve plates (Fig. 1).7,27 Aphids puncturing nearby SEs may therefore encounter less severe sieve-plate occlusion which results in facilitated settling and thus in increased population growth. Aggregation of feeding aphids would self-amplify population growth until a certain density is attained. Farther from the colonization site, this effect may be lost due to dilution. Stimulation of aphid feeding by aphid infestation was observed locally on potato by Myzus persicae and M. euphorbiae, respectively, 96 h after infestation.13 However, a similar effect was not observed for M. persicae on Arabidopsis thaliana where aphids induced premature leaf senescence and resistance 12 h after infestation,28 possibly induced by OGs.19As a speculation, OG induced Ca2+-influx into parenchyma cells adjacent to the salivary sheath activate Ca2+-induced signaling cascades via CaM,26,29 CDPKs,30,31 MAPKinases and reactive oxygen species (Fig. 2).32 Systemic resistance, induced by aphid infestation,12–14 is mediated by unknown compounds such as, e.g., salivary proteins, their degradation products, signal cascade products or volatiles.13 Compounds produced in CCs first have to pass the PPUs, while SE signaling elements can be directly transported via mass flow (Fig. 1).The question arises if aphids profit from induced resistance on local (cortex and parenchyma cells) and systemic (distant plant organs) levels as holds for suppression of defense in SEs. Possibly settling and subsequent spread of competing pathogens/herbivores (e.g., fungi or other piercing-sucking insects) are suppressed by induced defense. In this context it is intriguing to understand how aphids cope with the self-induced systemic resistance, which probably lasts over weeks.33 相似文献
62.
A 345-bp gene that encodes human bone morphogenetic protein-2 (hBMP-2) has been synthesized. The codon usage of the resulting gene was modified to include those triplets that are utilized in highly expressed Escherichia coli genes. The hBMP-2 gene was efficiently expressed in E. coli as a soluble and active protein. Since the recombinant hBMP-2 was readily solublized, no further solublization steps were required throughout purification. No additional tagging residues were introduced into the synthetic hBMP-2 gene product. The developed synthetic gene is a promising approach for scaling-up the soluble expression of hBMP-2. 相似文献
63.
The effect of surgery (insertion of an ileo-caecal cannula) and a subsequent parenteral treatment with oxytetracycline on the ileo-caecal and rectal coliform populations in 7 Swedish Yorkshire castrates were studied. Samples were collected during surgery as well as 3, 7, 14 and 20 days post surgery. The diversity of the enteric coliform flora was initially high both in the ileo-caecal ostium and in rectum. No alteration in the diversity of the enteric coliform flora was observed following surgery and treatment with oxytetracycline. As the insertion of ileo-caecal cannulas did not affect the intestinal coliform flora this study gives support to the use of this technique to mirror processes in the small intestine of pigs. Further, the diversity of the enteric coliform flora was unaffected by the parenteral treatment with oxytetracycline. 相似文献
64.
Structural maintenance of chromosomes (SMC) proteins, a family of conserved ATPases 总被引:3,自引:1,他引:2
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The structural maintenance of chromosomes (SMC) proteins are essential for successful chromosome transmission during replication and segregation of the genome in all organisms. SMCs are generally present as single proteins in bacteria, and as at least six distinct proteins in eukaryotes. The proteins range in size from approximately 110 to 170 kDa, and each has five distinct domains: amino- and carboxy-terminal globular domains, which contain sequences characteristic of ATPases, two coiled-coil regions separating the terminal domains and a central flexible hinge. SMC proteins function together with other proteins in a range of chromosomal transactions, including chromosome condensation, sister-chromatid cohesion, recombination, DNA repair and epigenetic silencing of gene expression. Recent studies are beginning to decipher molecular details of how these processes are carried out. 相似文献
65.
Jeom-Sook Lee Byung-Sun Ihm Du Sung Cho Dong-Yeob Son Jong-Wook Kim 《Journal of Plant Biology》2007,50(4):475-479
To identify and assess the distribution patterns of coastal dune vegetation along the eastern, southern, and western coasts
of South Korea, we investigated the plant communities and soil factors at 30 sites. In all, 12 communities on CCA (canonical
correspondence analysis) Axis 1 and 2 could be arranged into 3 groups: 1 ) 2 communities ofElymus mollis andIschaemum anthephoroides, with medium sand contents; 2) 6 communities ofCarex pumila, Carex kobomugi, Ixeris repens, Zoysia mac-rostachya, Calystegia soldanella, andVitex rotundifolia, with coarse sand contents; and 3) 4 communities ofLathyrus japonicus,Glehnia littoralis, Messerschmidia sibirica, andRosa rugosa, with very coarse sand contents. As identified via CCA ordination, the distribution of these groups was positively correlated
with soil particle sizes. 相似文献
66.
We studied how plant species distribution was regulated by the relationships between vegetation and soil factors on the southwestern
coast of South Korea. Vegetation was classified using two-way indicator species analysis (TWINSPAN), thereby producing four
vegetation groups that were linked to three habitat types. Two ordination techniques —; detrended correspondence analysis
(DCA) and canonical correspondence analysis (CCA) —; were applied to examine the relationships between vegetation and 12 edaphic
factors, including soil pH, water and osmotic potentials, moisture content, electrical conductivity, Cl and Na+ contents, total Kjeldahl nitrogen, and contents of organic matter, sand, silt, and clay. Results were similar for both types
of evaluations. According to DCA and CCA, the 23 communities tended to cluster into three types: salt swamp, salt marsh, and
sand dune. The first two canonical axes accounted for 14.9% of the community-soil factor relationship among communities. As
identified via CCA, the main gradients were soil-water relations and soil texture. 相似文献
67.
Hormonally regulated programmed cell death in barley aleurone cells 总被引:13,自引:0,他引:13
Cell death was studied in barley (cv Himalaya) aleurone cells treated with abscisic acid and gibberellin. Aleurone protoplasts incubated in abscisic acid remained viable in culture for at least 3 weeks, but exposure to gibberellin initiated a series of events that resulted in death. Between 4 and 8 days after incubation in gibberellin, >70% of all protoplasts died. Death, which occurred after cells became highly vacuolated, was manifest by an abrupt loss of plasma membrane integrity followed by rapid shrinkage of the cell corpse. Hydrolysis of DNA began before death and occurred as protoplasts ceased production of alpha-amylase. DNA degradation did not result in the accumulation of discrete low molecular weight fragments. DNA degradation and cell death were prevented by LY83583, an inhibitor of gibberellin signaling in barley aleurone. We conclude that cell death in aleurone cells is hormonally regulated and is the final step of a developmental program that promotes successful seedling establishment. 相似文献
68.
A multiple-regression model is presented for estimating the effect of major air pollutants on net photosynthetic rate (Pn)
ofQuercus mongolica leaves, of which visible injury is not shown. Photosynthetic capacity was found to be primarily a function of PPFD, air temperature
(T) and ambient ozone (O3) concentration. The negative direction of photosynthetic capacity response to O3 concentration indicates a potential growth reduction ofQ. mongolica due to ambient O3 concentration in the urban areas of Korea. The model was compared with a non-linear regression model including the same variables.
We assessed the contribution of variables to two two models of ambient O3 affecting Pn ofQ. mongolica leaves. The mean Pn difference between the models with and without ambient O3 in the multiple-regression was smaller than that in the non-linear regression. The relative contributions of ambient O3 to multiple-regression and non-linear regression were 12.6% and 5.6%, respectively. The results indicate that multiple-regression
models can be applicable for qualitative or quantitative assessment of the effect of air pollutants on Pn response of plant
leaves, of which visible injury may not be shown in situ. Also, the assessment of ecophysiological effects using numerical
models will have a degree of uncertainty associated with the measuring time/period of the field data used in the modelling,
as well as the numerical structure of the models. 相似文献
69.
Electrical and adaptive properties of rod photoreceptors in bufo marinus. I. Effects of altered extracellular Ca(2+) levels
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The effects of altering extracellular Ca(2+) levels on the electrical and adaptive properties of toad rods have been examined. The retina was continually superfused in control (1.6 mM Ca(2+)) or test ringer’s solutions, and rod electrical activity was recorded intracellularly. Low-calcium ringer’s (10(-9)M Ca(2+)) superfused for up to 6 min caused a substantial depolarization of the resting membrane potential, an increase in light-evoked response amplitudes, and a change in the waveform of the light-evoked responses. High Ca(2+) ringer’s (3.2 mM) hyperpolarized the cell membrane and decreased response amplitudes. However, under conditions of either low or high Ca(2+) superfusion for up to 6 min, in both dark-adapted and partially light-adapted states, receptor sensitivity was virtually unaffected; i.e., the V-log I curve for the receptor potential was always located on the intensity scale at a position predicted by the prevailing light level, not by Ca(2+) concentration. Thus, we speculate that cytosol Ca(2+) concentration is capable of regulating membrane potential levels and light-evoked response amplitudes, but not the major component of rod sensitivity. Low Ca(2+) ringer’s also shortened the period of receptor response saturation after a bright but nonbleaching light flash, hence accelerating the onset of both membrane potential and sensitivity recovery during dark adaptation.
Exposure of the retina to low Ca(2+) (10(-9)M) ringer’s for long periods (7-15 min) caused dark-adapted rods to lose responsiveness. Response amplitudes gradually decreased, and the rods became desensitized. These severe conditions of low Ca(2+) caused changes in the dark-adapted rod that mimic those observed in rods during light adaptation. We suggest that loss of receptor sensitivity during prolonged exposure to low Ca(2+) ringer’s results from a decrease of intracellular (intradisk) stores of Ca(2+); i.e., less Ca(2+) is thereby released per quantum catch.
相似文献70.