排序方式: 共有98条查询结果,搜索用时 15 毫秒
31.
Padilla O Calvo J Vilà JM Arman M Gimferrer I Places L Arias MT Pujana MA Vives J Lozano F 《Immunogenetics》2000,51(12):993-1001
CD5 is a member of the family of receptors which contain extracellular domains homologous to the type I macrophage scavenger receptor cysteine-rich (SRCR) domain. Here, we compare the exon/intron organization of the human CD5 gene with its mouse homologue, as well as with the human CD6 gene, the closest related member of the SRCR superfamily. The human CD5 gene spans about 24.5 kb and consists of at least 11 exons. These exons are conserved in size, number, and structure in the mouse CD5 homologue. No evidence for the biallelic polymorphism reported in the mouse could be found among a population of 100 individuals of different ethnic origins. The human CD5 gene maps to the Chromosome (Chr) 11q12.2 region, 82 kb downstream from the human CD6 gene, in a head-to-tail orientation, a situation which recalls that reported at mouse Chr 19. The exon/intron organization of the human CD5 and CD6 genes was very similar, differing in the size of intron 1 and the number of exons coding for their cytoplasmic regions. While several isoforms, resulting from alternative splicing of the cytoplasmic exons, have been reported for CD6, we only found evidence of a cytoplasmic tailless CD5 isoform. The conserved structure of the CD5 and CD6 loci, both in mouse and human genomes, supports the notion that the two genes may have evolved from duplication of a primordial gene. The existence of a gene complex for the SRCR superfamily on human Chr 11q (and mouse Chr 19) still remains to be disclosed. 相似文献
32.
Ekhiñe Artola Saioa Cruchaga Idoia Ariz Jose Fernando Moran María Garnica Fabrice Houdusse José María Garcia Mina Ignacio Irigoyen Berta Lasa Pedro María Aparicio-Tejo 《Plant Growth Regulation》2011,63(1):73-79
The use of urea as an N fertilizer has increased to such an extent that it is now the most widely used fertilizer in the world.
However, N losses as a result of ammonia volatilization lead to a decrease in its efficiency, therefore different methods
have been developed over the years to reduce these losses. One of the most recent involves the use of urea combined with urease
inhibitors, such as N-(n-butyl) thiophosphoric triamide (NBPT), in an attempt to delay the hydrolysis of urea in the soil. The aim of this study was
to perform an in-depth analysis of the effect that NBPT use has on plant growth and N metabolism. Wheat plants were cultivated
in a greenhouse experiment lasting 4 weeks and fertilized with urea and NBPT at different concentrations (0, 0.012, 0.062,
0.125%). Each treatment was replicated six times. A non-fertilized control was also cultivated. Several parameters related
with N metabolism were analysed at the end of growth period. NBPT use was found to have visible effects, such as a transitory
yellowing of the leaf tips, at the end of the first week of treatment. At a metabolic level, plants treated with the inhibitor
were found to have more urea in their tissues and a lower amino acid content, lower glutamine synthetase activity, and lower
urease and glutamine synthetase content at the end of the study period, whereas their urease activity seemed to have recovered
by this stage. 相似文献
33.
34.
Maria Sporbert Petr Keil Gunnar Seidler Helge Bruelheide Ute Jandt Svetlana Aćić Idoia Biurrun Juan Antonio Campos Andraž Čarni Milan Chytrý Renata Ćušterevska Jürgen Dengler Valentin Golub Florian Jansen Anna Kuzemko Jonathan Lenoir Corrado Marcenò Jesper Erenskjold Moeslund Aaron Pérez-Haase Solvita Rūsiņa Urban Šilc Ioannis Tsiripidris Vigdis Vandvik Kiril Vasilev Risto Virtanen Erik Welk 《Journal of Biogeography》2020,47(10):2210-2222
35.
Tomáš Peterka Michal Hájek Martin Jiroušek Borja Jiménez‐Alfaro Liene Aunina Ariel Bergamini Daniel Dítě Ljuba Felbaba‐Klushyna Ulrich Graf Petra Hájková Eva Hettenbergerová Tatiana G. Ivchenko Florian Jansen Natalia E. Koroleva Elena D. Lapshina Predrag M. Lazarević Asbjørn Moen Maxim G. Napreenko Paweł Pawlikowski Zuzana Plesková Lucia Sekulová Viktor A. Smagin Teemu Tahvanainen Annett Thiele Claudia Biţǎ‐Nicolae Idoia Biurrun Henry Brisse Renata Ćušterevska Els De Bie Jörg Ewald Úna FitzPatrick Xavier Font Ute Jandt Zygmunt Kącki Anna Kuzemko Flavia Landucci Jesper E. Moeslund Aaron Pérez‐Haase Valerijus Rašomavičius John S. Rodwell Joop H.J. Schaminée Urban Šilc Zvjezdana Stančić Milan Chytrý 《应用植被学》2017,20(1):124-142
36.
Sandra Acosta Cinzia Lavarino Raquel Paris Idoia Garcia Carmen de Torres Eva Rodríguez Helena Beleta Jaume Mora 《BMC developmental biology》2009,9(1):12-14
Background
Neuroblastic tumors (NBT) derive from neural crest stem cells (NCSC). Histologically, NBT are composed by neuroblasts and Schwannian cells. In culture, neuroblastic (N-), substrate-adherent (S-) and intermediate phenotype (I-) cell subtypes arise spontaneously. 相似文献37.
Martín-Pagola A Pérez-Nanclares G Ortiz L Vitoria JC Hualde I Zaballa R Preciado E Castaño L Bilbao JR 《Immunogenetics》2004,56(8):549-554
MHC class I chain-related gene A (MICA), a putative independent susceptibility gene in autoimmune diseases, encodes a surface protein present in epithelial cells that binds to NKG2D, an activating receptor of NK, and T cells, and could function as a stress-inducible activator of the innate immune response. There is no evidence of a long-term implication of MICA in the celiac autoimmune process. However, it could be that gliadin activation of MICA occurs only during the initial stages of the disease. In order to determine whether MICA is activated in response to gliadin in patients with celiac disease (CD), small intestinal mucosa biopsy samples from ten long-standing celiac patients on a gluten-free diet and from five non-celiac individuals were incubated with and without gliadin for 4 h. Total RNA was purified and MICA, IFNG and NKG2D mRNA were quantified by fluorescent real-time RT-PCR. Expression levels were calculated relative to GAPDH. MICA expression was detected in both patients and controls, but incubation with gliadin induced a strong increase in samples from the treated CD group compared with the non-CD controls (P=0.028), while no differences were observed for IFNG or NKG2D mRNA levels. The gliadin-provoked over-expression of MICA in normalized tissues from CD patients suggests a role for this stress-induced activator of the immune response in the early stages of organ-specific autoimmune destruction, probably preceding the onset of inflammation. 相似文献
38.
Alicia Muela Maite Orruño M. Luz Alonso Marta Pazos Inés Arana Rosa M. Alonso Rosa M. Jiménez Idoia Garaizabal M. Itxaso Maguregui Isabel Barcina 《Ecological Indicators》2011,11(2):431-437
The operation of an activated sludge wastewater treatment plant (WWTP) was assessed by determining the removal efficiency of treatment processes. For that purpose, different physicochemical and microbiological parameters, including total, active and culturable bacteria, were measured along a year. Values of both physicochemical and microbiological parameters were significantly reduced by biological treatment but no relation between these groups of parameters was obtained. Besides, results suggest that microbiological parameters were more sensitive than physicochemical ones to changes in WWTP conditions (i.e. influent quality). Additionally, high percentages of noncultured (>88%) and noncultured remaining active (until 50%) cells were detected before and after treatment. Therefore, ISO standards adopted to enumerate indicator bacteria by Spanish legislation, and based on culturability, underestimate the bacterial population present in wastewater. We have compared two culture-independent methods for bacterial enumeration, microscopy and flow cytometry (FCM) and results indicate that FCM provides a rapid and automated quantification in WWTP. We conclude that microbiological parameters are essential to monitor the correct WWTP operation and we propose quantification of active bacteria as indicator of wastewater microbiological quality. 相似文献
39.
Alonso-Fauste I Andrés M Iturralde M Lampreave F Gallart J Alava MA 《Journal of Proteomics》2012,75(10):3015-3030
Acute phase proteins (APP) have been identified in whey and sera from healthy and mastitis cows through the proteomic analysis using two-dimensional electrophoresis (2-DE) coupled with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). Although normal and mastitis serum samples show relatively similar protein composition, marked differences in expression levels and patterns can be observed. Conversely, normal and mastitis whey showed a very different composition, likely due to extravasation of blood proteins to the mammary gland. Different isoforms from the most abundant protein in milk, casein, were detected in both normal and mastitis whey. Other proteins, such as lactotransferrin, were only detected in the inflamed animal samples. Immunoglobulins showed different patterns but not increased levels in the inflamed whey. Also, many cellular proteins in mastitis cow's whey, that were absent from healthy cow's milk. They are responsible for the great change in composition between normal and mastitis whey, especially those which exert a biological function related to immune defense. Data collected in this work are of interest for gaining information about physiological changes in protein patterns in different fluids and, the correspondent modifications as result of an acute phase process in farm. This article is part of a Special Issue entitled: Proteomics: The clinical link. 相似文献
40.
Conjugation of genetically engineered protein phosphatases to magnetic particles for okadaic acid detection 总被引:1,自引:0,他引:1
Garibo D Devic E Marty JL Diogène J Unzueta I Blázquez M Campàs M 《Journal of biotechnology》2012,157(1):89-95
This work presents the functional characterisation of a protein phosphatase 2A (PP2A) catalytic subunit obtained by genetic engineering and its conjugation to magnetic particles (MPs) via metal coordination chemistry for the subsequent development of assays for diarrheic lipophilic marine toxins. Colorimetric assays with free enzyme have allowed the determination of the best enzyme activity stabiliser, which is glycerol at 10%. They have also demonstrated that the recombinant enzyme can be as sensitive towards okadaic acid (OA) (LOD = 2.3 μg/L) and dinophysistoxin-1 (DTX-1) (LOD = 15.2 μg/L) as a commercial PP2A and, moreover, it has a higher operational stability, which makes possible to perform the protein phosphatase inhibition assay (PPIA) with a lower enzyme amount. Once conjugated to MPs, the PP2A catalytic subunit still retains its enzyme activity and it can also be inhibited by OA (LOD = 30.1 μg/L). 相似文献