Effect of the charge of the interface surface on the structure and activity of trypsin in reversed mycelles

The effect of the sign and value of the charge of the interface surface on the catalytic activity of trypsin in systems of reversed mycelles was investigated. n-Butanol was used for the modification of the phase interface in dispersions of reversed mycelles based on anionic sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and cationic cetyltrimethylammonium bromide (CTAB). A direct correlation between changes in the state of reversed mycelles and the structure of solubilized enzyme under the action of butanol was obtained. It was shown that the enzyme activity is determined by the quantity of butanol solubilized by the reversed mycelles.