全文获取类型
收费全文 | 1436篇 |
免费 | 74篇 |
出版年
2022年 | 17篇 |
2021年 | 32篇 |
2020年 | 15篇 |
2019年 | 12篇 |
2018年 | 29篇 |
2017年 | 20篇 |
2016年 | 44篇 |
2015年 | 73篇 |
2014年 | 81篇 |
2013年 | 107篇 |
2012年 | 118篇 |
2011年 | 114篇 |
2010年 | 62篇 |
2009年 | 45篇 |
2008年 | 89篇 |
2007年 | 89篇 |
2006年 | 68篇 |
2005年 | 68篇 |
2004年 | 67篇 |
2003年 | 45篇 |
2002年 | 53篇 |
2001年 | 17篇 |
2000年 | 19篇 |
1999年 | 9篇 |
1998年 | 16篇 |
1997年 | 10篇 |
1996年 | 14篇 |
1995年 | 4篇 |
1994年 | 7篇 |
1993年 | 9篇 |
1992年 | 7篇 |
1991年 | 17篇 |
1990年 | 8篇 |
1989年 | 13篇 |
1988年 | 7篇 |
1987年 | 10篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 14篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1974年 | 5篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1949年 | 3篇 |
1934年 | 2篇 |
1919年 | 2篇 |
排序方式: 共有1510条查询结果,搜索用时 78 毫秒
121.
Bobrowska-Hägerstrand M Wróbel A Rychlik B Ohman I Hägerstrand H 《Molecular membrane biology》2007,24(5-6):485-495
The presence of human multidrug resistance protein 1 (MRP1/ABCC1) in the human erythrocyte membrane is well established. In the present study, flow cytometric monitoring is introduced to identify MRP1 as the main transporter of 2',7'-bis-(3-carboxypropyl)-5-(and-6)-carboxyfluorescein (BCPCF) in the erythrocyte membrane and to facilitate inhibition and kinetic studies of MRP1-mediated transport. The ATP-dependent transport of BCPCF into human erythrocyte inside-out vesicles and, for comparison, into MRP1-expressing Sf9 cell membrane inside-out vesicles were studied. The MRP1-specific monoclonal antibody, QCRL-3 and the MRP1 inhibitor, MK-571 strongly decreased the uptake of BCPCF into both erythrocyte and MRP1-expressing Sf9 cell membrane inside-out vesicles. The inhibition profiles of cyclosporin A, verapamil, benzbromarone, and probenecid in erythrocyte membrane vesicles were typical for MRP1-mediated transport. Furthermore, kinetic constants K(m) and V(max) of BCPCF transport into erythrocyte membrane inside-out vesicles were determined in the absence and in the presence of selected inhibitors (MK-571, cyclosporin A, benzbromarone and verapamil). The presented results identified MRP1 as the major transporter of BCPCF in the human erythrocyte membrane and showed for the first time that the active transport of fluorescent substrate into inside-out vesicles can be monitored by flow cytometry. 相似文献
122.
123.
124.
A new epigonid fish, Epigonus cavaticus, is described on the basis of eight specimens (59.2–69.5 in standard length: SL) collected from a cave at depth 20 m, southern
fringing reef of Ngemelis Island, Palau. The species differs from other congeners by having minute teeth on both jaws, no
opercular spine, pyloric caeca 7–8, gill rakers 25–27, total pored lateral line scales 48–50, dorsal fin rays VII-I, 10–11
(mode VII-I, 10), pectoral fin rays 16, vertebrae 10 + 15, body depth 21.4–25.0% SL, pectoral fin length 22.7–24.6% SL, eye
diameter 44.4–47.5% head length: HL, upper jaw length 40.2–42.5% HL. Four paratypes (63.7–66.8 mm SL) of the new species are
female with mature gonads, it is the smallest in size at sexual maturity among the congeners. 相似文献
125.
Alpi A Amrhein N Bertl A Blatt MR Blumwald E Cervone F Dainty J De Michelis MI Epstein E Galston AW Goldsmith MH Hawes C Hell R Hetherington A Hofte H Juergens G Leaver CJ Moroni A Murphy A Oparka K Perata P Quader H Rausch T Ritzenthaler C Rivetta A Robinson DG Sanders D Scheres B Schumacher K Sentenac H Slayman CL Soave C Somerville C Taiz L Thiel G Wagner R 《Trends in plant science》2007,12(4):135-136
126.
Carlo Alviggi Alberto Revelli Paola Anserini Antonio Ranieri Luigi Fedele Ida Strina Marco Massobrio Nicola Ragni Giuseppe De Placido 《Reproductive biology and endocrinology : RB&E》2007,5(1):45-7
Background
Multifollicular ovarian stimulation (MOS) is widely used in IVF and the compliance to treatment is deeply influenced by the tolerability of the medication(s) used and by the ease of self-administration. This prospective, controlled, randomised, parallel group open label, multicenter, phase III, equivalence study has been aimed to compare the clinical effectiveness (in terms of oocytes obtained) and tolerability of subcutaneous (s.c.) self-administered versus classical intramuscular (i.m.) injections of Merional, a new highly-purified hMG preparation. 相似文献127.
Said Aoufouchi Annie De Smet Frédéric Delbos Camille Gelot Ida Chiara Guerrera Jean-Claude Weill Claude-Agnès Reynaud 《Molecular and cellular biology》2015,35(17):3059-3070
Mice derived from the 129 strain have a nonsense codon mutation in exon 2 of the polymerase iota (Polι) gene and are therefore considered Polι deficient. When we amplified Polι mRNA from 129/SvJ or 129/Ola testes, only a small fraction of the full-length cDNA contained the nonsense mutation; the major fraction corresponded to a variant Polι isoform lacking exon 2. Polι mRNA lacking exon 2 contains an open reading frame, and the corresponding protein was detected using a polyclonal antibody raised against the C terminus of the murine Polι protein. The identity of the corresponding protein was further confirmed by mass spectrometry. Although the variant protein was expressed at only 5 to 10% of the level of wild-type Polι, it retained de novo DNA synthesis activity, the capacity to form replication foci following UV irradiation, and the ability to rescue UV light sensitivity in Polι−/− embryonic fibroblasts derived from a new, fully deficient Polι knockout (KO) mouse line. Furthermore, in vivo treatment of 129-derived male mice with Velcade, a drug that inhibits proteasome function, stabilized and restored a substantial amount of the variant Polι in these animals, indicating that its turnover is controlled by the proteasome. An analysis of two xeroderma pigmentosum-variant (XPV) cases corresponding to missense mutants of Polη, a related translesion synthesis (TLS) polymerase in the same family, similarly showed a destabilization of the catalytically active mutant protein by the proteasome. Collectively, these data challenge the prevailing hypothesis that 129-derived strains of mice are completely deficient in Polι activity. The data also document, both for 129-derived mouse strains and for some XPV patients, new cases of genetic defects corresponding to the destabilization of an otherwise functional protein, the phenotype of which is reversible by proteasome inhibition. 相似文献
128.
Komlavi Anani Afanou Anne Straumfors Asbj?rn Skogstad Ajay P. Nayak Ida Skaar Linda Hjeljord Arne Tronsmo Wijnand Eduard Brett James Green 《Applied and environmental microbiology》2015,81(17):5794-5803
Submicronic fungal fragments have been observed in in vitro aerosolization experiments. The occurrence of these particles has therefore been suggested to contribute to respiratory health problems observed in mold-contaminated indoor environments. However, the role of submicronic fragments in exacerbating adverse health effects has remained unclear due to limitations associated with detection methods. In the present study, we report the development of an indirect immunodetection assay that utilizes chicken polyclonal antibodies developed against spores from Aspergillus versicolor and high-resolution field emission scanning electron microscopy (FESEM). Immunolabeling was performed with A. versicolor fragments immobilized and fixed onto poly-l-lysine-coated polycarbonate filters. Ninety percent of submicronic fragments and 1- to 2-μm fragments, compared to 100% of >2-μm fragments generated from pure freeze-dried mycelial fragments of A. versicolor, were positively labeled. In proof-of-concept experiments, air samples collected from moldy indoor environments were evaluated using the immunolabeling technique. Our results indicated that 13% of the total collected particles were derived from fungi. This fraction comprises 79% of the fragments that were detected by immunolabeling and 21% of the spore particles that were morphologically identified. The methods reported in this study enable the enumeration of fungal particles, including submicronic fragments, in a complex heterogeneous environmental sample. 相似文献
129.
Pressure pain threshold of the cervico‐facial muscles in healthy elderly people: the role of gender,age and dominance
下载免费PDF全文
![点击此处可从《Gerodontology》网站下载免费的PDF全文](/ch/ext_images/free.gif)