IGF-1 Reduces BACE-1 Expression in PC12 Cells via Activation of PI3-K/Akt and MAPK/ERK1/2 Signaling Pathways

Insulin-like growth factor 1 (IGF-1) stimulates α-secretase processing of amyloid precursor protein (APP) and decreases Aβ production. Little is known about the relationship between IGF-1 and β-site amyloid precursor protein cleaving enzyme 1 (BACE-1), the protease essential for the production of β-amyloid peptides (Aβ). Here, we investigated the effect of IGF-1 on BACE-1 in PC12 cells. Quantitative polymerase chain reaction analysis and western blot showed that treatment of cells with IGF-1 significantly decreased the levels of BACE-1 mRNA and protein. Furthermore, IGF-1 increased the phosphorylation of Akt and ERK1/2. The presence of the phosphatidylinositol 3-kinase (PI3-K) inhibitor LY294002 and the mitogen-activated protein kinase kinases (MEK) inhibitor PD98059 blocked the effect of IGF-1 on BACE-1. Our data indicated that IGF-1-induced reduction of BACE-1 might involve the PI3-K/Akt and MAPK/ERK1/2 signaling pathways.     

Production,characteristics and applications of the cell-bound phytase of <Emphasis Type=Italic>Pichia anomala</Emphasis>

Among several yeasts isolated from dried flowers of Woodfordia fruticosa, Pichia anomala produced a high titre of cell-bound phytase. The optimization of fermentation variables led to formulation of media and selection of cultural variables that supported enhanced phytase production. The enzyme productivity was very high in fed batch fermentation in air-lift fermentor as compared to that in stirred tank fermentor. Amelioration in the cell-bound phytase activity was observed when yeast cells were permeabilized with Triton-X-100. The enzyme is thermostable and acid stable with broad substrate specificity, the characteristics that are desirable for enzymes to be used in the animal feed industry. The phytase-encoding gene was cloned and sequenced. The 3D structure of the enzyme was proposed by comparative modeling using phytase of Debaryomyces occidentalis (50% sequence identity) as template. When broiler chicks, and fresh water and marine fishes were fed with the feed supplemented with yeast biomass containing phytase, improvement in growth and phosphorus retention, and decrease in the excretion of phosphorus in the faeces were recorded. The cell-bound phytase of P. anomala could effectively dephytinize wheat flour and soymilk.     

Comparison of dynamic responses of cellular metabolites in <Emphasis Type=Italic>Escherichia coli</Emphasis> to pulse addition of substrates

We conducted an integrated study of cell growth parameters, product formation, and the dynamics of intracellular metabolite concentrations using Escherichia coli with genes knocked out in the glycolytic and oxidative pentose phosphate pathway (PPP) for glucose catabolism. We investigated the same characteristics in the wild-type strain, using acetate or pyruvate as the sole carbon source. Dramatic effects on growth parameters and extracellular and intracellular metabolite concentrations were observed after blocking either glycolytic breakdown of glucose by inactivation of phosphoglucose isomerase (disruption of pgi gene) or pentose phosphate breakdown of glucose by inactivation of glucose-6-phosphate dehydrogenase (disruption of zwf gene). Reducing power (NADPH) was mainly produced through PPP when the pgi gene was knocked out, while NADPH was produced through the tricarboxylic acid (TCA) cycle by isocitrate dehydrogenase or NADP-linked malic enzyme when the zwf gene was knocked out. As expected, when the pgi gene was knocked out, intracellular concentrations of PPP metabolites were high and glycolytic and concentrations of TCA cycle pathway metabolites were low. In the zwf gene knockout, concentrations of PPP metabolites were low and concentrations of intracellular glycolytic and TCA cycle metabolites were high.     

New,rare and noteworthy lichens in the Giant Mountains

A commented list of 19 particularly interesting and noteworthy lichen species collected recently in the Giant Mts (Karkonosze, Krkonoše) is given. Most of them were found on the basalt outcrop in the western wall of the Mały Śnieżny Kocioł cirque (germ. Kleine Schneegrube), which is the most valuable and most conserved area in this mountain range. Two species are new to Poland: Lecanora gangaleoides and Lecidea praenubila. Umbilicaria cinereorufescens is reported for the first time from the Sudety Mts as a whole. Three species are new for the Giant Mts: Buellia ocellata, Diplotomma lutosum and Fuscidea praeruptorum. Eight species: Aspicilia microlepis, Belonia russula, Caloplaca crenularia, C. nivalis, Gyalecta biformis, Koerberiella wimmeriana, Miriquidica complanata and Polyblastia cruenta were re-discovered after over 100 years on the historical locality in the Mały Śnieżny Kocioł cirque.     

Technology transfer in biotechnology in Central Europe

A research commercialisation programme mapped the Slovak self-governing region of Bratislava for existing and potential spin-off companies. Identification and evaluation analyses of the technology transfer in biotechnology-oriented research were executed. Results of the study confirmed the quality of present level of science but a lack of commercialisation of professional services on the other hand. Financial plans for suggested spin-off business models were predicted by licensed BioBiz™ software programme.     

Comparative analyses of <Emphasis Type=Italic>Astacus leptodactylus</Emphasis> morphological characteristics from Croatia and Armenia

Narrow-clawed crayfish, Astacus leptodactylus is a native European freshwater crayfish species, also distributed in Croatian freshwater systems belonging to the Black Sea drainage. Its taxonomical status is still in the process of change and discussion, and the data on morphological, molecular, ecological and zoogeographical characteristics of this species are scarce. Therefore, comparative analyses of morphological characteristics were applied with the aim to contribute to the knowledge on the morphometrical and meristical characteristics of A. leptodactylus. Recent research proved that measurements of a large number of morphometrical characteristics, in combination with multivariate statistical analysis, could provide a good instrument for identification and differentiation between populations. In this research altogether 143 animals were analysed (121 from two Croatian populations and 22 from Armenia). 22 morphometrical characteristics and 4 meristical characteristics, per crayfish, were measured. It was found that males and females differ between populations in measured meristical and morphometrical characteristics. None of recorded meristical characteristics proved itself to be reliable characteristic for distinguishing populations. From measured morphometrical characteristics the most discriminant characteristics for separating males from different populations were those describing carapace shape and for females those that describe shape of the claws.     

Seed production of cut-leaved teasel (<Emphasis Type=Italic>Dipsacus laciniatus</Emphasis>) in central Missouri

Cut-leaved teasel is an invasive weed in Missouri that reduces the diversification of native species along roadsides and impairs traffic visibility. Teasel is a biennial and grows as a rosette in the first year and flowers the second year. Reproduction is only by seed. Field studies were conducted in 2004 and 2005 at two locations to assess the seed production of cut-leaved teasel. From a natural stand, fifteen plants were tagged at the onset of flowering. Selected plants included those considered growing in a group and those growing alone; a plant was considered alone when no other plant was adjacent for at least 60 cm. Whenever a seedhead completed flowering, it was covered with a cellophane bag and harvested one month later. Linear regression was used to correlate the weight of seeds from a single seedhead and number of seeds to estimate the total seed production per seedhead. The number of seedheads per plant varied from 3 to 56. On average, plants growing alone had 64% more seedheads per plant than plants occurring in a group. Seed numbers in the primary seedhead ranged from 511 to 1,487. Total seed production per plant ranged from 1,309 to 33,527. Seed production was 61% greater for plants growing alone versus those growing in a group and was more prolific in 2005 than in 2004. In addition, seed production per plant varied between locations for plants growing alone, but seed yield per plant was similar for plants growing in groups. Colonization of teasel in new areas is facilitated by higher seedhead numbers per plant and total seed production compared to reproduction of plants in areas of intraspecific competition.     

Tropical Vine Growth and the Effects on Forest Succession: A Review of the Ecology and Management of Tropical Climbing Plants

The climbing habit has evolved independently in many plant taxa, offering vines the ability to compete with non-climbing vegetation for resources such as light, nutrients, and water. This review examines the structural and functional characteristics that allow climbing plants to (1) achieve widespread dispersal, (2) transport large amounts of water throughout vessels, (3) maintain high photosynthesis levels through a large leaf area to biomass ratio, (4) achieve rapid vertical and horizontal expansion by fast growth rates and various climbing mechanisms and (5) survive and recover from disturbances. Due to the competitive effects of vines on trees, management of vine growth is used to preserve tropical timber plantations, combat invasive weeds, and promote rainforest recovery. In order to sustainably manage the vines into the future, it is necessary to understand the mechanisms by which they can alter tropical forest succession and the impacts of various management techniques.