HPLC separation and characterization of tick-borne encephalitis and equine Venezuela encephalomyelitis viral proteins]

Homogeneous (according to PAGE) capsid and surface viral proteins were isolated from concentrated purified suspensions of tick-borne encephalitis and Venezuelan equine encephalomyelitis viruses by one-stage reversed-phase HPLC. The amino acid composition and the sequences of their N-terminal parts were determined.     

Role of bone marrow glycosaminoglycans in the reaction of the hematopoietic tissue to extreme conditions

The authors studied medullary glucosaminoglycans of rats and mice in extreme effects (hypoxia, hyperbaric oxygenation, inflammation, radiation, cooling, hemorrhage). Four types of reaction were determined which cause different changes of proliferation of hematopoietic cells. Hematopoiesis stimulation is accompanied by regular change of types of reaction of hematopoietic microenvironment. Its disturbance affects the changes of hematopoiesis and blood regeneration rate. It was shown that the increase of neutral glucosaminoglycans concentrations in bone marrow depended on the play ground and proliferative activity of erythroid sprout and that of acid glucosaminoglycans--primarily of granulocytic sprout.     

Phylogeny and antiquity of M macrohaplogroup inferred from complete mt DNA sequence of Indian specific lineages

Background  

Analysis of human complete mitochondrial DNA sequences has largely contributed to resolve phylogenies and antiquity of different lineages belonging to the majorhaplogroups L, N and M (East-Asian lineages). In the absence of whole mtDNA sequence information of M lineages reported in India that exhibits highest diversity within the sub-continent, the present study was undertaken to provide a detailed analysis of this macrohaplogroup to precisely characterize and unravel the intricate phylogeny of the lineages and to establish the antiquity of M lineages in India.     

Characteristics of the acute phase reaction in humans with various types of autonomic nervous system regulation during simulated hyperthermia

Depending on the type of autonomous regulation, differences in basic levels of interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF alpha) were revealed under conditions of hyperthermia in healthy subjects aged 19-21. A parasympathetic type of autonomous regulation corresponded to higher initial levels of proinflammatory cytokinesis, whereas a dominating sympathetic type corresponded to lower levels of the IL-1 beta and TNF alpha. The subjects with the latter type of regulation revealed an increase in the IL-1 beta TNF alpha combined with a higher heat tolerance. The subjects with the former type of regulation revealed a lower heat tolerance. The increase in the alpha2-macroglobulin appeared to be a most typical acute phase response of the human body to hyperthermia.     

High performance liquid chromatography and characterization of structural proteins of the influenza virus

Constituent proteins of human influenza virus A have been separated by reverse-phase HPLC on Polysil ODS-500. Their homogeneity is confirmed by the data of amino acid composition, Edman analysis and gel electrophoresis.