Patterns of Gd- allele distribution in Azerbaijan. IV. The incidence and polymorphism of erythrocytic glucose-6-phosphate dehydrogenase deficiency in the settlement of Kobi, Apsheron District

The frequency and polymorphism of erythrocyte G6Pd deficiency were studied in schoolchildren of Koby settlement, Apsheron district (n = 469). The total frequency of Gd- alleles was 3.6%. Out of 10 hemizygotes found 9 had Gd- alleles of the III class and 1 had Gd- allele of the II class. The mutant enzyme was isolated from 10 ml venous blood samples and studied according to WHO program. Three different mutant forms of G6PD were found, neither of them having been earlier described. One of these alleles was common for the population studied (n = 6).     

Comparative study of the distribution of semisynthetic cephalosporins in the body of rats]

Distribution of 6 cephalosporin antibiotics, i. e. cephaloridin, cephalotin, cephradin cephacetryl, cephazolin and cephapyrin for parenteral use was studied comparatively on rats. The studies showed that all the above cephalosporins were well absorbed into the blood after intramuscular administration. The highest serum levels were achieved with the use of cephozolin. Still, its levels in the animal organs were mainly not higher and sometimes even lower than those provided by the other antibiotics. The highest levels of cephalosporins were detected in the kidneys. Cephalotin, cephapyrin and cephacetryl differed by the character of their distribution in the rats from the other 3 antibiotics: the levels of cephalotin and cephapyrin in the heart, spleen and muscles were lower than those of the other cephalosporins; sometimes they were even not detected in these organs; cephacetryl was not found in these organs. The levels of these 3 antibiotics in the kidneys were lower than those of the other cephalosporins. Cephalotin, cephacetryl and sometimes cephapyrin were not detected in the rat liver. None of the cephalosporins was found in the brain tissue.     

Pili (fimbriae) of the causative agents of plague and pseudotuberculosis: their detection in the hemagglutination reaction, the passive hemagglutination reaction and in an immunoenzyme analysis system

Newly developed serological methods for the detection of pili in the passive hemagglutination (PHA) test with the use of immunoglobulin erythrocytic diagnosticum and in the enzyme immunoassay (EIA) with the use of specific immunoglobulins labeled with horse radish peroxidase have been found to exceed the method of detecting pili, based on the determination of their hemagglutinating activity, in sensitivity and specificity. Besides, the PHA test and EIA have proved to be capable of detecting low molecular fragments of pili, obtained by sonication and having lost their hemagglutinating activity.