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211.
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A G Iakovlev 《Biulleten' eksperimental'no? biologii i meditsiny》1988,105(1):67-68
It is suggested to use epithelial cells from the inner cheek surface as a source of high molecular individual genomic human DNA to be used in Southern hybridization. The method efficiency has been proved experimentally. 相似文献
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V P Iakovlev 《Antibiotiki》1978,23(6):526-533
Rifamycin pharmacokinetics was studied on experimental animals after the antibiotic administration by various routes. Parenteral use of the antibiotic resulted in its high levels in rats and rabbits. Irrespective of the administration route, i. e. intravenous, intramuscular or oral rifamycin satisfactorily penetrated into the rat tissues. The highest antibiotic levels were found in the animal liver. In small amounts the antibiotic was excreted with the urine (about 6 per cent for 4 hours). The extrarenal clearance of rifamycin was lower than the plasmic clearance only by 3 per cent and higher than the kidney clearance almost by 40 times. Rifamycin was bound in close amounts by the blood serum of humans, oxen and rabbits, i. e. by 68, 64 and 56 per cent respectively. The rat organ homogenates bound the antibiotic by 34--72 per cent. 相似文献
215.
L V Ponomareva V I Iakovlev N P Tsvetkova V G Krunchak 《Antibiotiki i khimioterapii͡a》1999,44(1):11-13
A new composition of the nutrient medium for cultivation of the tetracycline-producing organism was developed with the fermentative hydrolysate of the tetracycline production mycelial waste as a source of nitrogen: 0.02 to 0.04 g/l by amino nitrogen. The use of the medium made it possible to increase the tetracycline yield by 5 to 25 per cent, to exclude cornsteep liquor from the medium composition, to provide a more efficient recovery of the waste and to significantly decrease the environment pollution. 相似文献
216.
A Ia Evtushenko A I Iakovlev L A Shaliakin 《Biulleten' eksperimental'no? biologii i meditsiny》1985,99(3):284-286
Experiments on cats were made to study the cardiac output and its main fractions supplying blood to the epi- and subdiaphragmal parts of the body during the postresuscitation period. The phenomenon of early postresuscitation centralization of circulation was established, correlating with the gravity of the sustained terminal state in the severity and duration. 相似文献
217.
E A Vorontsov N L Lifshits N I Mal'tsev V A Iakovlev 《Biokhimii?a (Moscow, Russia)》1976,41(2):248-254
Hydrazide group of 4-substituted NAD analogues is shown to interact with functional groups of substrate-binding site in double complexes with pig muscle lactate dehydrogenase (isoenzyme M4). The lactic acid residue, which is structurally incorporated into NAD analogue, improves slightly the binding of dinucleotide, while 2,2,6,6-tetramethylpiperidine-1-oxyl residue considerably decreases the firmless of binding. The comparison of the inhibitory ability of oxamate, incotinic acid hydraxide and their spin-labelled derivatives indicates the restricted and stiff sizes of a substrate-binding site. 相似文献
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