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991.
Elizabeth Franklin Damian Evans Ann Thornton Chris Moody Iain Green Anita Diaz 《Arthropod-Plant Interactions》2017,11(1):79-88
Invasive carnivorous plant species can impact the native invertebrate communities on which they prey. This article explores the predation of native UK bumblebees (Bombus spp.) by the invasive pitcher plant species Sarracenia purpurea and discusses the potential effect of S. purpurea on native bumblebees. Specifically, it evaluates whether the extent to which bumblebees are captured varies (i) over successive years, (ii) across June and July, (iii) with density of distribution of pitchers or (iv) with bumblebee gender. Pitcher contents were examined from an established population of Sarracenia purpurea growing in Dorset, UK. Results show that the total extent to which bumblebees were captured differed over the years 2012–2014 inclusive. A 1-year study in 2013 showed that more bumblebees were caught in July than in June and more bumblebees were captured when pitchers grew at high density. Results from 2013 also showed that more pitchers caught more than one bumblebee than would be expected based on a normal probability distribution and that this phenomenon affects female and male bumblebees equally. We discuss possible reasons for these results including that the bumblebees may be using S. purpurea as a resource. Further work is required to establish the exact underpinning mechanisms and the relative roles of plant and bumblebee behaviour within the relationship. Such interaction complexity may have consequences for consideration in invasive carnivorous plant management. 相似文献
992.
993.
Mattaj IW 《Nature reviews. Molecular cell biology》2004,5(1):65-69
Although the interphase nuclear envelope is continuous with the endoplasmic reticulum, it is distinct from the endoplasmic reticulum in both form and composition. In metazoans, the nuclear envelope breaks down during mitosis and is thought to be completely reabsorbed by the endoplasmic reticulum. How these near neighbours are sorted out at the end of mitosis is an interesting mystery. 相似文献
994.
Cell division requires the assembly of a microtubule-based spindle which captures and segregates sister chromatids. But how is this spindle broken down once chromosome segregation is complete? New evidence implicates a highly conserved AAA-ATPase in spindle disassembly at the end of mitosis. 相似文献
995.
Little is known about the genetic control of mitochondrial morphology and dynamics in higher plants. We used a genetic screen involving fluorescence microscopic analysis of ethyl methane sulphonate (EMS)-mutated Arabidopsis thaliana seedlings expressing GFP targeted to mitochondria to isolate eight mutants displaying distinct perturbations of the normal mitochondrial morphology or distribution. We describe five mutants with distinct and unique mitochondrial phenotypes, which map to five different loci, not previously implicated in mitochondrial behaviour in plants. We have used a combination of forward and reverse genetics to identify one of the genes, friendly mitochondria (FMT), a homologue of the CluA gene of Dictyostelium discoideum, which is involved in the correct distribution of mitochondria in the cell. The five mutants constitute a powerful resource to aid our understanding of mitochondrial dynamics in higher plants. 相似文献
996.
Young I 《Nature biotechnology》2003,21(3):339-340
997.
Leung BP Conacher M Hunter D McInnes IB Liew FY Brewer JM 《Journal of immunology (Baltimore, Md. : 1950)》2002,169(12):7071-7077
Transferring collagen-pulsed, bone marrow-derived dendritic cells (DCs) into congenic DBA/1 recipient mice produced arthritis in joints adjacent to the site of DC transfer and could be inhibited by treatment with TNF antagonists. Disease was Ag specific, as transfer of control, unpulsed DCs, or DCs pulsed with OVA did not produce arthritis. In contrast to other experimental arthritis models, DC-induced arthritis localized to the site of injection and did not spontaneously generalize to uninvolved joints, despite the demonstration of circulating collagen-reactive T cells. Similarly, transfer of T cells primed by collagen/DCs was not sufficient to produce arthritis in recipient mice. In collagen/DC-primed mice however, disease could be induced in uninvolved joints by local administration of noncollagen-pulsed DCs and this could be reduced through TNF inhibition. Similarly, injection of collagen/DC-primed mice with low-dose TNF also resulted in local induction of arthritis, as did administration of TNF to mice receiving T cells from collagen/DC but not OVA/DC-primed mice. Thus, we have demonstrated for the first time that administration of collagen-pulsed mature DCs is sufficient for the induction of arthritis. Furthermore, this disease process is mediated through both adaptive and innate effects of DCs; first, priming of autoreactive T cells and, second, induction of local inflammation via mediators such as TNF. 相似文献
998.
Effects of the N2144S mutation on backbone dynamics of a TB-cbEGF domain pair from human fibrillin-1 总被引:2,自引:0,他引:2
Yuan X Werner JM Lack J Knott V Handford PA Campbell ID Downing AK 《Journal of molecular biology》2002,316(1):113-125
The calcium-binding epidermal growth factor-like (cbEGF) module and the transforming growth factor beta-binding protein-like (TB) module are the two major structural motifs found in fibrillin-1, the extracellular matrix (ECM) protein defective in the Marfan syndrome (MFS). An MFS-causing mutation, N2144S, which removes a calcium ligand in cbEGF32, does not detectably affect fibrillin-1 biosynthesis, rate of secretion, processing, or deposition of reducible fibrillin-1 into the ECM. Since the residue at position 2144 is normally engaged in calcium ligation, it is unable to mediate intermolecular interactions. We have shown previously that this mutation does not affect the folding properties of the TB or cbEGF domains in vitro, but does decrease calcium-binding in cbEGF and TB-cbEGF domain constructs. Here, we use NMR spectroscopy to probe the effects of the N2144S mutation on backbone dynamic properties of TB6-cbEGF32. Analysis of the backbone (15)N relaxation data of wild-type TB6-cbEGF32 has revealed a flexible inter-domain linkage. Parallel dynamics analysis of the N2144S mutant has shown increased flexibility in the region joining the two domains as well as in the calcium-binding site at the N terminus of cbEGF32. This research demonstrates that a small change in peptide backbone flexibility, which does not enhance proteolytic susceptibility of the domain pair, is associated with an MFS phenotype. Flexibility of the TB-cbEGF linkage is likely to contribute to the biomechanical properties of fibrillin-rich connective tissue microfibrils, and may play a role in the microfibril assembly process. 相似文献
999.
Nearly all establishments in the UK regulated under the Animals (Scientific Procedures) Act 1986 had introduced an ethical review process (ERP) within 9 months of its formal requirement, although quite a high proportion of more junior staff were not familiar with it. A significant proportion of those questioned believed that the ERP has improved the quality (particularly the ethical quality) of project licences. A smaller proportion of respondents believed that the ERP has had a beneficial impact on animal work and training. Nearly all the respondents viewed animal care and accommodation as good or excellent. 相似文献
1000.
Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p 总被引:32,自引:0,他引:32
Cheeseman IM Anderson S Jwa M Green EM Kang Js Yates JR Chan CS Drubin DG Barnes G 《Cell》2002,111(2):163-172
The Aurora kinase Ipl1p plays a crucial role in regulating kinetochore-microtubule attachments in budding yeast, but the underlying basis for this regulation is not known. To identify Ipl1p targets, we first purified 28 kinetochore proteins from yeast protein extracts. These studies identified five previously uncharacterized kinetochore proteins and defined two additional kinetochore subcomplexes. We then used mass spectrometry to identify 18 phosphorylation sites in 7 of these 28 proteins. Ten of these phosphorylation sites are targeted directly by Ipl1p, allowing us to identify a consensus phosphorylation site for an Aurora kinase. Our systematic mutational analysis of the Ipl1p phosphorylation sites demonstrated that the essential microtubule binding protein Dam1p is a key Ipl1p target for regulating kinetochore-microtubule attachments in vivo. 相似文献