全文获取类型
收费全文 | 183篇 |
免费 | 17篇 |
出版年
2022年 | 2篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2016年 | 9篇 |
2015年 | 6篇 |
2014年 | 4篇 |
2013年 | 4篇 |
2012年 | 10篇 |
2011年 | 14篇 |
2010年 | 2篇 |
2009年 | 5篇 |
2008年 | 8篇 |
2007年 | 7篇 |
2006年 | 9篇 |
2005年 | 9篇 |
2004年 | 8篇 |
2003年 | 15篇 |
2002年 | 12篇 |
2001年 | 16篇 |
2000年 | 13篇 |
1999年 | 9篇 |
1998年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1980年 | 2篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1970年 | 3篇 |
1966年 | 2篇 |
1962年 | 1篇 |
1914年 | 3篇 |
1913年 | 1篇 |
1912年 | 1篇 |
1911年 | 1篇 |
1909年 | 1篇 |
排序方式: 共有200条查询结果,搜索用时 250 毫秒
11.
Vang T Abrahamsen H Myklebust S Horejsí V Taskén K 《The Journal of biological chemistry》2003,278(20):17597-17600
Raft-associated Csk controls signaling through the T cell receptor (TCR) and was mainly anchored to Cbp/PAG (phosphoprotein associated with glycosphingolipid-enriched membrane domains). Treatment of cells with the cAMP-elevating agent prostaglandin E(2) (PGE(2)) augmented the level of Cbp/PAG phosphorylation with a concomitant increase in amounts of Csk bound to Cbp/PAG. While TCR-triggering resulted in transient dissociation of Csk from Cbp/PAG/rafts allowing TCR-induced tyrosine phosphorylation to occur, pretreatment with PGE(2) reduced Csk dissociation upon TCR triggering. This correlated with lowered TCR-induced phosphorylation of CD3 zeta-chain and linker for activation of T cells. Moreover, competition of endogenous Csk from lipid rafts abolished PGE(2)-mediated inhibition of TCR-induced zeta-chain phosphorylation and activation of the nuclear factor of activated T cells (NFAT) activator protein 1 (AP-1). Finally, raft-associated Csk already activated via Cbp/PAG binding, gained additional increase in phosphotransferase activity upon protein kinase A-mediated phosphorylation of Csk. We propose that cAMP regulates Csk via both spatial and enzymatic mechanisms, thereby inhibiting signaling through the TCR. 相似文献
12.
Lee HY Srinivas H Xia D Lu Y Superty R LaPushin R Gomez-Manzano C Gal AM Walsh GL Force T Ueki K Mills GB Kurie JM 《The Journal of biological chemistry》2003,278(26):23630-23638
Cancer cells in which the PTEN lipid phosphatase gene is deleted have constitutively activated phosphatidylinositol 3-kinase (PI3K)-dependent signaling and require activation of this pathway for survival. In non-small cell lung cancer (NSCLC) cells, PI3K-dependent signaling is typically activated through mechanisms other than PTEN gene loss. The role of PI3K in the survival of cancer cells that express wild-type PTEN has not been defined. Here we provide evidence that H1299 NSCLC cells, which express wild-type PTEN, underwent proliferative arrest following treatment with an inhibitor of all isoforms of class I PI3K catalytic activity (LY294002) or overexpression of the PTEN lipid phosphatase. In contrast, overexpression of a dominant-negative mutant of the p85alpha regulatory subunit of PI3K (Deltap85) induced apoptosis. Whereas PTEN and Delta85 both inhibited activation of AKT/protein kinase B, only Deltap85 inhibited c-Jun NH2-terminal kinase (JNK) activity. Cotransfection of the constitutively active mutant Rac-1 (Val12), an upstream activator of JNK, abrogated Deltap85-induced lung cancer cell death, whereas constitutively active mutant mitogen-activated protein kinase kinase (MKK)-1 (R4F) did not. Furthermore, LY294002 induced apoptosis of MKK4-null but not wild-type mouse embryo fibroblasts. Therefore, we propose that, in the setting of wild-type PTEN, PI3K- and MKK4/JNK-dependent pathways cooperate to maintain cell survival. 相似文献
13.
Eide T Taskén KA Carlson C Williams G Jahnsen T Taskén K Collas P 《The Journal of biological chemistry》2003,278(29):26750-26756
Protein kinase A (PKA)-anchoring protein AKAP95 is localized to the nucleus in interphase, where it primarily associates with the nuclear matrix. A yeast two-hybrid screen for AKAP95 interaction partners identified the minichromosome maintenance (MCM) 2 protein, a component of the pre-replication complex. AKAP95-MCM2 interaction was mapped to residues 1-195 of AKAP95 and corroborated by glutathione S-transferase precipitation and immunoprecipitation from chromatin. Disruption of AKAP95-MCM2 interaction with an AKAP95-(1-195) peptide within HeLa cell nuclei abolishes initiation of DNA replication in G1 phase and the elongation phase of replication in vitro without affecting global nuclear organization or import. Disruption of the C-terminal zinc finger of AKAP95 reduces efficiency of replication initiation. Disruption of the PKA-binding domain does not impair replication in G1- or S-phase nuclei, whereas a PKA inhibitor affects the initiation but not the elongation phase of replication. Depleting AKAP95 from nuclei partially depletes MCM2 and abolishes replication. Recombinant AKAP95 restores intranuclear MCM2 and replication in a dose-dependent manner. Our results suggest a role of AKAP95 in DNA replication by providing a scaffold for MCM2. 相似文献
14.
15.
16.
Chlorophyll fluorescence measurements have a wide range of applications from basic understanding of photosynthesis functioning
to plant environmental stress responses and direct assessments of plant health. The measured signal is the fluorescence intensity
(expressed in relative units) and the most meaningful data are derived from the time dependent increase in fluorescence intensity
achieved upon application of continuous bright light to a previously dark adapted sample. The fluorescence response changes
over time and is termed the Kautsky curve or chlorophyll fluorescence transient. Recently, Strasser and Strasser (1995) formulated
a group of fluorescence parameters, called the JIP-test, that quantify the stepwise flow of energy through Photosystem II,
using input data from the fluorescence transient. The purpose of this study was to establish relationships between the biochemical
reactions occurring in PS II and specific JIP-test parameters. This was approached using isolated systems that facilitated
the addition of modifying agents, a PS II electron transport inhibitor, an electron acceptor and an uncoupler, whose effects
on PS II activity are well documented in the literature. The alteration to PS II activity caused by each of these compounds
could then be monitored through the JIP-test parameters and compared and contrasted with the literature. The known alteration
in PS II activity of Chenopodium album atrazine resistant and sensitive biotypes was also used to gauge the effectiveness and sensitivity of the JIP-test. The information
gained from the in vitro study was successfully applied to an in situ study. This is the first in a series of four papers. It shows that the trapping parameters of the JIP-test were most affected
by illumination and that the reduction in trapping had a run-on effect to inhibit electron transport. When irradiance exposure
proceeded to photoinhibition, the electron transport probability parameter was greatly reduced and dissipation significantly
increased. These results illustrate the advantage of monitoring a number of fluorescence parameters over the use of just one,
which is often the case when the FV/FM ratio is used.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
17.
Orstavik S Eide T Collas P Han IO Taskén K Kieff E Jahnsen T Skålhegg BS 《Biology of the cell / under the auspices of the European Cell Biology Organization》2000,92(1):27-37
Previously, we have identified and characterized nuclear AKAP95 from man which targets cyclic AMP (cAMP)-dependent protein kinase (PKA)-type II to the condensed chromatin/spindle region at mitosis. Here we report the cloning of a novel nuclear protein with an apparent molecular mass of 95 kDa that is similar to AKAP95 and is designated HA95 (homologous to AKAP95). HA95 cDNA sequence encodes a protein of 646 amino acids that shows 61% homology to the deduced amino acid sequence of AKAP95. The HA95 gene is located on chromosome 19p13.1 immediately upstream of the AKAP95 gene. Both HA95 and AKAP95 genes contain 14 exons encoding similar regions of the respective proteins, indicating a previous gene duplication event as the origin of the two tandem genes. Despite their apparent similarity, HA95 does not bind RII in vitro. HA95 contains a putative nuclear localization signal in its N-terminal domain. It is localized exclusively into the nucleus as demonstrated in cells transfected with HA95 fused to either green fluorescence protein or the c-myc epitope. In the nucleus, the HA95 protein is found as complexes directly associated with each other or indirectly associated via other nuclear proteins. In interphase, HA95 is co-localized with AKAP95, but the two proteins are not biochemically associated. At metaphase, both proteins co-localize with condensed chromosomes. The similarity in sequence and localization of HA95 and AKAP95 suggests that the two molecules constitute a novel family of nuclear proteins that may exhibit related functions. 相似文献
18.
Force WR Glass AA Benedict CA Cheung TC Lama J Ware CF 《The Journal of biological chemistry》2000,275(15):11121-11129
Lymphotoxin-beta receptor (LTbetaR), a member of the tumor necrosis factor receptor superfamily, is essential for the development and organization of secondary lymphoid tissue. Wild type and mutant LTbetaR containing successive truncations of the cytoplasmic domain were investigated by retrovirus-mediated gene transfer into HT29.14s and in 293T cells by transfection. Wild type receptors accumulated in perinuclear compartments and enhanced responsiveness to ligand-induced cell death and ligand-independent activation of NFkappaB p50 dimers. Coimmunoprecipitation and confocal microscopy mapped the TRAF3 binding site to amino acids PEEGDPG at position 389. However, LTbetaR truncated at position Pro(379) acted as a dominant positive mutant that down-modulated surface expression and recruited TRAF3 to endogenous LTbetaR. This mutant exhibited ligand-independent cell death and activated NF-kappaB p50 dimers. By contrast, truncation at Gly(359) created a dominant-negative mutant that inhibited ligand-induced cell death and activation of NF-kappaB p50/p65 heterodimers. This mutant also blocked accumulation of wild type receptor into perinuclear compartments, suggesting subcellular localization may be crucial for signal transduction. A cryptic TRAF-independent NF-kappaB activating region was identified. These mutants define discrete subregions of a novel proline-rich domain that is required for subcellular localization and signal transduction by the LTbetaR. 相似文献
19.
HSP72 can protect cells from heat-induced apoptosis by accelerating the inactivation of stress kinase JNK 总被引:8,自引:2,他引:6
下载免费PDF全文
![点击此处可从《Cell stress & chaperones》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The major heat shock protein Hsp72 prevents heat-induced apoptosis. We have previously demonstrated that transiently expressed Hsp72 exerts its anti-apoptotic effect by suppressing the activity of stress-kinase JNK, an early component of the apoptotic pathway initiated by heat shock. On the other hand, constitutive expression of Hsp72 does not lead to suppression of heat-induced JNK activation, yet still efficiently prevents apoptosis. To address this apparent contradiction, we studied the effects of constitutively expressed Hsp72 on activation of JNK and apoptosis in Rat-1 fibroblasts. We found that the level of heat-induced apoptosis directly correlated with the duration rather than the magnitude of JNK activity following heat shock. Constitutively expressed Hsp72 strongly reduced the duration of JNK while it did not suppress initial JNK activation. These effects were due to Hsp72-mediated acceleration of JNK dephosphorylation. Addition of vanadate to inhibit JNK phosphatase activity completely prevented the anti-apoptotic action of Hsp72. Therefore, suppression of heat-induced apoptosis by Hsp72 could be fully accounted for by its effects on JNK activity. 相似文献
20.
Makkinje A Quinn DA Chen A Cadilla CL Force T Bonventre JV Kyriakis JM 《The Journal of biological chemistry》2000,275(23):17838-17847