首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   901719篇
  免费   91539篇
  国内免费   293篇
  993551篇
  2018年   8732篇
  2017年   8331篇
  2016年   11665篇
  2015年   14705篇
  2014年   17598篇
  2013年   25473篇
  2012年   28564篇
  2011年   29520篇
  2010年   20189篇
  2009年   18644篇
  2008年   26468篇
  2007年   27582篇
  2006年   25838篇
  2005年   24793篇
  2004年   24615篇
  2003年   23712篇
  2002年   23307篇
  2001年   39106篇
  2000年   38819篇
  1999年   30999篇
  1998年   11392篇
  1997年   11548篇
  1996年   10930篇
  1995年   10101篇
  1994年   9738篇
  1993年   9863篇
  1992年   25331篇
  1991年   24978篇
  1990年   24264篇
  1989年   23740篇
  1988年   21810篇
  1987年   20989篇
  1986年   19519篇
  1985年   19482篇
  1984年   16022篇
  1983年   13924篇
  1982年   10495篇
  1981年   9480篇
  1980年   8869篇
  1979年   14953篇
  1978年   11879篇
  1977年   10689篇
  1976年   10127篇
  1975年   11447篇
  1974年   12354篇
  1973年   12201篇
  1972年   11237篇
  1971年   10091篇
  1970年   8834篇
  1969年   8673篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
121.
Structure of the spectrin-actin binding site of erythrocyte protein 4.1   总被引:9,自引:0,他引:9  
The complete primary structure of the functional site of erythrocyte protein 4.1 involved in spectrin-actin associations has been determined. The sequence of this domain, which contains 67 amino acids and has a molecular mass of 8045 daltons, has been obtained by NH2-terminal sequence analysis of an 8-kDa chymotryptic peptide, three endoproteinase lysine C-cleaved peptides and two peptides obtained by Staphylococcus aureus protease V8 cleavage. All peptides including the 8-kDa domain peptide were purified by reverse-phase high performance liquid chromatography. Antibodies against two different synthetic peptides of the 8-kDa domain are able to inhibit the association between protein 4.1, spectrin, and F-actin, corroborating that the 8-kDa domain is responsible for the formation of a ternary complex. A computer search of the 8-kDa sequence with the National Biomedical Research Foundation database did not detect any significant homologies to known sequences. Protein 4.1 is not related to any known proteins and may represent a new protein superfamily.  相似文献   
122.
Monosomy 21: a new case confirmed by in situ hybridization   总被引:4,自引:0,他引:4  
Summary A new case of total monosomy 21 in a newborn is described. The diagnosis was first made using the cytogenetic data; it was then confirmed by the dosage of copper-superoxide dismutase (SOD-1) which showed a 50% decrease. In situ hybridization with a probe previously assigned to chromosome 21 was used to rule out the possibility of a partial monosomy with an unbalanced reciprocal translocation.  相似文献   
123.
1. Because L-asparagine augments insulin release evoked by L-leucine, the metabolism of these two amino acids was investigated in rat pancreatic islets. 2. L-Leucine inhibited the uptake and deamidation of L-asparagine, but failed to exert any obvious primary effect upon the further catabolism of aspartate derived from exogenous asparagine. 3. L-Asparagine augmented the oxidation of L-leucine, and effect possibly attributable to activaion of 2-ketoisocaproate dehydrogenase. 4. The association of L-asparagine and L-leucine exerted a sparing action on the utilization of endogenous amino acids, so that the integrated rate of nutrients oxidation was virtually identical in the sole presence of L-leucine and simultaneous presence of L-asparagine and L-leucine, respectively. 5. It is proposed that the enhancing action of L-asparagine upon insulin release evoked by L-leucine is attributable to an increased generation rate of cytosolic NADPH rather than any increase in nutrients oxidation.  相似文献   
124.
125.
Temperature-sensitive (ts) mutations of the G101 phage were isolated after mutagenesis with hydroxylamine. A complementation analysis of 61ts mutants showed that these mutants may be divided into at least 12 complementation groups. Twots mutants probably originated in genes which control lytic functions of the G101 phage. It was shown by three factor crosses that all of the 12ts mutations tested are localized on that side of the “c” region where the probablecI repressor gene is positioned. Sevents mutations is closely linked to thecI 26 clear marker, three exhibit a closer linkage and two do not exhibit any linkage withcI. All mutations isolated until now can be arrange linearly. According to the present knowledge the preliminary genetic map of the G101 phage is linear.  相似文献   
126.
127.
With the use of a patch-clamp technique in the whole-cell configuration, we studied the effects of pinacidil and its fluorine derivatives on A-type potassium current (I A) through the membrane of pyramidal neurons of the rat hippocampus. Hydrogen peroxide (10 mM) exerted no influence on the rate of inactivation ofI A; therefore, this current is probably mediated by Shal Kv4.2 potassium channels. Pinacidil demonstrated the properties of a weakI A blocker: in the 500 μM concentration it blocked about 45% of the current, while 50 μM of pinacidil fluorine derivatives were capable of blocking up to 30% ofI A. The effects of pinacidil and its derivatives showed no dependence on the stimulating potential. A similar pattern of the effects of pinacidil fluorine derivatives, which are an order of magnitude stronger than those of pinacidil itself, allows us to suppose that the imine nitrogen of the tested compounds is significantly more involved in the molecular interaction with the site of an A-type potassium channel than the pyridine nitrogen.  相似文献   
128.
129.
130.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号